摘要： 从基因库中调取已完成测序的纤维素合成酶CesA(Cellulose synthase)基因序列和氨基酸序列,共涉及15个物种的89个基因,基于以上氨基酸序列,应用常用的系统发生关系树生成软件MEGA3.1,做出这89个基因的系统发生关系树。综合已知的模式植物CesA基因的功能(仅指初生壁或次生壁形成特异性),可推测某些未知功能基因的可能功能。研究还发现绿竹CesA基因与玉米和大麦CesA基因在系统发生关系和同源性方面关系密切。CesA一级结构可变区中半胱氨酸、丙氨酸、甘氨酸和丝氨酸等氨基酸的含量变化较大,在同一位点半胱氨酸多是与丙氨酸、丝氨酸和缬氨酸发生相互替换。
关键词:
纤维素合成酶,
基因功能,
系统发生关系
Abstract: Phylogenetic trees of cellulose synthase(CesA)gene from 89 sequences of CesA in 15 species including plants and lichen and alga published on the Genebank were generated by MEGA3.1.Based on the information of model plant CesA′s gene regulated the biosynthesis of primary or secondary wall,the function of some unknown genes could be speculated from the trees.The study also found that the CesA genes of Bambusa oldhamii shared the high identity and close phylogenetic relationships with that of maize and barley.The changes of Cys,Ala,Gly and Ser content were greater than that of others in the variable region of CesA′s primary structure.The exchanges between Cys and Ala or Ser or Val in the same site were found in the variable region.
Key words:
Cellulose synthase,
Cane function,
Phylogenetic relationship
中图分类号:
李益, 胡尚连, 卢学琴, 蒋瑶, 黄胜雄, 李向前. 植物纤维素合成酶基因的进化分析[J]. 华北农学报, 2008, 23(2): 101-105. doi: 10.7668/hbnxb.2008.02.023.
LI Yi, HU Shang-lian, LU Xue-qin, JIANG Yao, HUANG Sheng-xiong, LI Xiang-qian. Evolution Analysis of the Plant Cellulose Synthase(CesA)Gene Family[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(2): 101-105. doi: 10.7668/hbnxb.2008.02.023.