植物基因功能研究新方法

doi:10.7668/hbnxb.2014.S1.023

华北农学报 ›› 2014, Vol. 29 ›› Issue (S1): 109-113. doi: 10.7668/hbnxb.2014.S1.023

所属专题： 生物技术；

植物基因功能研究新方法

彭陈¹, 王瑞², 李万昌³, 郭士伟¹

1. 江苏省农业科学院农业生物技术研究所, 江苏省农业生物学重点实验室, 江苏南京 210014;
2. 江苏省农业科技实业总公司, 江苏南京 210014;
3. 河南师范大学生命科学院, 河南新乡 453007

收稿日期:2014-10-09 出版日期:2014-12-28
通讯作者: 郭士伟(1971-),男,河南汝州人,副研究员,硕士,主要从事水稻真菌病害研究。
作者简介:彭陈(1988-),男,安徽合肥人,硕士,主要从事水稻真菌病害研究。
基金资助:
公益性行业(农业)科研专项(201303102)

New Method for Studying Plant Gene Function

PENG Chen¹, WANG Rui², LI Wan-chang³, GUO Shi-wei¹

1. Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Provincial Key Laboratory of Agrobiology, Nanjing 210014, China;
2. Jiangsu Agricultural Scientific Industry Co., Nanjing 210014, China;
3. College of Life Sciences, Henan Normal University, Xinxiang 453007, China

Received:2014-10-09 Published:2014-12-28

摘要/Abstract

摘要： TALEN(Transcription activator-like effector nuclease)和CRISPR/Cas系统(Clustered regularly interspaced short palindromic repeats,CRISPR.CRISPR-associated,Cas)是近年来发现的基因功能研究的新方法,且已经成功地在动物、植物及微生物等多个领域得到成功应用。它们可以对DNA双链进行切割,从而启动细胞内的DNA修复机制,以此来实现对靶位点的基因修饰。相较于传统方法,它们具有特异性高、操作简便及打靶效率高等特点。主要从结构、功能及作用机制等方面阐述这2种新基因功能的研究方法。

关键词: 基因功能, TALEN, CRISPR/Cas系统

Abstract: TALEN(Transcription activator-like effector nuclease) CRISPR/Cas system(Clustered regularly interspaced short palindromic repeats,CRISPR.CRISPR-associated,Cas) have recently emerged as new technique for targeted genome modifications.They have been successfully applied in many fields such as animals,plants and microorganism.They can generate a double-strand break(DSB).Repair of these DSBs by intracellular DNA repair mechanisms enables to achieve the target gene modification.Compared with traditional method,TALEN and CRISPR/Cas system were stronger specificity,manipulated more easily and higher targeting efficiency.Here we report the construction,function and mechanism of the TALEN and CRISPR/Cas system.

Key words: Gene function, TALEN, CRISPR/Cas system

中图分类号:

彭陈, 王瑞, 李万昌, 郭士伟. 植物基因功能研究新方法[J]. 华北农学报, 2014, 29(S1): 109-113. doi: 10.7668/hbnxb.2014.S1.023.

PENG Chen, WANG Rui, LI Wan-chang, GUO Shi-wei. New Method for Studying Plant Gene Function[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(S1): 109-113. doi: 10.7668/hbnxb.2014.S1.023.

http://www.hbnxb.net/CN/Y2014/V29/IS1/109

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