摘要： 旨在为研究猪T细胞受体分子结构与功能,并首次在国内克隆了猪T细胞受体α链基因.以GenBank上登载的猪T细胞受体(T cell receptor,TCR)α链(AB08988)为参考序列,从甘肃合作猪外周血液淋巴细胞中用RT-PCR的方法克隆了TCR-α链基因(pTCR-α).结果表明:克隆的TCR-α基因具有完整的ORF,全长819 bp,编码22个氨基酸,预测蛋白分子量为30 kDa,含18个强碱性氨基酸,23个强酸性氨基酸,8个疏水性氨基酸和103个极性氨基酸,含有一段20个氨基酸的信号肽序列;与GenBank上登载的TCR-α参考序列(AB08988)的同源性为94.6%,与其他具有完整ORF的猪TCR-α相比,同源性为54.4%～80.3%.推测可能是一新的TCR-α基因转录本.同时,应用生物信息学技术分析了其基因序列及编码蛋白的结构特征,这为猪T细胞受体分子结构与功能的研究奠定了基础.

关键词: 猪T细胞受体&alpha, 链基因, 基因克隆, 序列分析, 生物信息学

Abstract: In order to study molecular structure and function of T cell receptor,porcine T cell receptor alpha chain gene was cloned from peripheral-blood lymphocyte(PBL) of Gansu hezuo pig at the first time in domesdic.The sequence analysis shows that the ORF of total pTCR-α gene is 819 bp in length,encoding 272 amino acids with a signal peptide composed of 20 amino acids.The protein molecular weight is about 30 kDa,with 18 Strongly Basic(+) Amino Acids,23 Strongly Acidic(-) Amino Acids,87 Hydrophobic Amino Acids and 102 Polar Amino Acids.The homology of nucleotide sequence of porcine TCR-α with the referenced sequence(AB087988) is up to 94.6%.Compared with other porcine genes of TCR-α published in GenBank,the homology is about 54.4%-80.3%.It may be a novel transcript of TCR-α family.In order to research molecular structure and function of T cell receptor,this study also uses bioinformatics to analyze its gene sequences and structural feature of encoding protein.

Key words: Porcine T cell receptor alpha chain gene(pTCR-α), Gene cloning, Sequence analysis, Bioinformatics

中图分类号: 

• Q785