摘要： 从腐烂朽木及附近的土壤采样, 分离到25株具有纤维素分解能力的菌株, 其中LY16菌株的纤维素酶活最高。采用形态学观察及分子生物学方法初步鉴定其为里氏木霉(Trichoderma reesei)。通过碳氮源优化等试验进行产酶条件研究, 得出该菌株最佳的产酶条件, 培养基配方为(g/L):微晶纤维素10, 麸皮40, 蛋白胨4, 尿素12, KH₂PO₄2, MgSO₄.7H₂O.1, CaCl₂.2H₂O.1, FeSO₄0.01, MnSO₄0.004 5, CoCl₂0.0036, ZnSO₄0.0035, 培养温度30℃、装液量50mL, 转速200 r/min, 培养时间为72 h。优化后在该条件下, CMC酶活为202.6 U/mL, FPA酶活最大53.2 U/mL。

关键词: 里氏木霉, 纤维素酶, 鉴定, 产酶条件

Abstract: Samples were collected from rotten wood and soil nearby, and 25 strains that can decompose the cellulose were separated, among which the strain LY16 has the strongest cellulase activity. It was identified as Trichoderma reesei by morphological observation and the molecular biological methods. By carrying out the carbon-nitrogen source and other experiments, we have obtained the optimal conditions in which this strain produces enzyme. The culture compositioon (g/L) contained :avicel 10, wheat bran 40, peptone 4, urea 12, KH₂PO₄2, MgSO₄ ·7H₂O.1, CaCl₂·2H₂O.1, FeSO₄0.01, MnSO₄0.004 5, CoCl₂0.0036, ZnSO₄0.0035, Culture temperature was 30 ℃, Liquid volume in flask was 50 mL, rotational speed was 200 r/min, culture time 72 h. In this condition, the CMCase was 202.6 U/mL, and the FPase was 53.2 U/mL.

Key words: Trichoderma reesei, Cellulase, Identification, Fermentation conditions

中图分类号: 

• TQ925