摘要： 根据GenBank中脂肪酸延长酶基因fae1序列(AY 888037)设计了PCR扩增引物，以野芥总DNA为模板进行扩增得到了特异扩增条带。测序结果表明，该片段长1651bp，序列分析表明其氨基酸编码区为55～1575bp，不含内含子序列，共编码506个氨基酸。该基因序列已提交GenBank，登录号为FJ870905。BLASTn分析结果表明，野芥中fae1基因与其他芸薹属品系的fae1基因核苷酸同源性为76%～98%；BLASTp分析结果表明，野芥中的FAE1与油菜中FAE1存在32个位点差异，其中部分差异可能导致了芥酸含量的不同。

关键词: 野芥, 芥酸, 脂肪酸延长酶基因1(fae1), 基因克隆, 序列分析

Abstract: PCR primers were designed according to the fae1 (Fatty acid elongase 1.fae1) DNA sequence of Brassica napus Zhongshuang 9 reported in GenBank (Accession No.AY888037),PCR was carried out with genomic DNA of Sinapis arvensis as template and the specific fragment was purified and cloned.Result of sequencing showed that the fragment was 1651 bp and analysis of sequence indicated that the coding region was 1521 bp and encoded 506 amino acids.Results of BLASTn demonstrated that the fae1 from Sinapis arvensis has 76%-98% nucleotide similarity with genes cloned from Brassica species,and BLASTp demonstrated that 32 amino acids residue substitutions were found between Sinapis arvensis and <>iBrassica species,some sequence changes could explain lines with different erucic acid content.

Key words: Sinapis arvensis L., Erucic acid, Fatty acid elongase 1(fae1), Gene cloning, Sequence analysis

中图分类号: 

• Q785