摘要： 以葱细胞质雄性不育系CA及其同核异质保持系CB为材料,通过分析花蕾发育过程中活性氧(O2)产生速率、丙二醛(MDA)含量以及抗氧化酶(超氧化物歧化酶、过氧化氢酶、过氧化物酶)活性变化研究了活性氧产生与清除的动态变化.结果表明:不育系花蕾败育初期,活性氧产生速率持续增加至败育盛期达最高值,而丙二醛含量变化则从中蕾期开始大量积累至败育盛期接近最高值,表现一定的滞后性.两系比较,从小蕾期开始不育系活性氧产生速率和丙二醛含量显著高于保持系.受活性氧产生的诱导作用,败育始期超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性均呈增加趋势,但在败育盛期SOD、CAT活性明显下降,显著低于保持系.而POD活性则在中蕾期前低于保持系,之后显著高于保持系,造成雄性器官发育过程中IAA的亏缺.因此,葱花蕾发育过程中O2代谢失调和MDA过量积累以及保护酶活性下降或功能转变可能是导致雄性不育的重要因素.
关键词:
葱,
雄性不育系,
活性氧代谢,
保护酶
Abstract: Welsh onion ( A. fistulosum) buds of Cytoplasmic Male2Sterile (CMS) line and its maintainer line at differ2 ent developmental stages were employeded as experimental materials to reveal relationships between CMS and metabolisms of reactive oxygen species (ROS) by measuring productions of superoxide anion radical (O2 H ),content of malonadehyde (MDA) and activities of antioxidant enzymes ( superoxide,catalase and peroxidase enzymes). The result showed that at abortion preliminery stage,O2 H production rate of CMS buds increased singnificantly and reached the top at abortion peak stage,whereas content of MDA accumulated excessively from middle2bud stage to reach the top approximately at abortion peak stage. Simultaneously,both O2 H production rate and MDA content of CMS were higher significantly than those of its maintainer from the little2bud stage. Induced by O2 H productions,superoxide,catalase and peroxidase enzyme (SOD,CAT and POD) activities of CMS increased from abortion preliminery stage,however,SOD and CAT activities,which decreased continuously from the middle2bud stage,were lower than those of its maintainer at abortion peak stage. Compared with SOD and CAT,POD activity was lower than that of its maintainer before middle2bud stage,and then was higher signifi2 cantly,which resulted in scarcity of IAA of male2sterile buds.Accordingly,unbalanced O2 H metablisms,excessive accumulations of MDA and decline of protective enzymes′activities or change of some protective enzyme′s functions maybe the important reasons that cause CMS.
Key words:
Welsh onion,
Cytoplasmic male2sterile (CMS) line,
Metabolisms of reactive oxygen species (ROS),
Pro2 tective enzymes
中图分类号:
苗锦山, 杨文才, 李美芹, 沈火林. 葱细胞质雄性不育与花蕾发育过程中活性氧代谢关系研究[J]. 华北农学报, 2009, 24(4): 92-95. doi: 10.7668/hbnxb.2009.04.019.
MIAO Jin-shan, YANG Wen-cai, LI Mei-qin, SHEN Huo-lin,. Studies on the Relationships between Cytoplasmic Male Sterility and Reactive Oxygen Species Metablisms during Development of Welsh Onion(A.fistulosum)Buds[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 92-95. doi: 10.7668/hbnxb.2009.04.019.