摘要： 采用垂直平板不连续聚丙烯酰胺凝胶电泳技术分别对蒙古马、三河马及纯血马的8种血液蛋向进行多态性检测.计算了各位点的基因型频率、基因频率、遗传杂合度,并通过聚类分析探讨了群体间的亲缘关系和遗传距离.结果表明:种血液蛋白(酶)位点在不同程度上呈现出多态.平均杂合度分别为:蒙古马0.378 2,三河马0.395 6和纯血马0.257 1.聚类分析表明,蒙古马与三河马间的亲缘关系最近,与纯血马的亲缘关系相对较远.通过分析不同马品种的遗传关系,为中国马遗传资源的保护与育种提供依据.
关键词:
马,
血液蛋白,
遗传多态性,
聚类分析
Abstract: Eight types of blood proteins in serum samples fromMongolian horse,Sanhe horse and Thoroughbred horse were detected on polymorphism by vertical plate discontinuous polyacrylamide gel electrophoresis (PAGE).Genotype frequencies,gene frequency and genetic heterozygosity of these sites were calculated,and the relationship and the genetic distance between the groups were discussed through cluster analysis.The results indicated that eight blood protein (enzyme) sites were of polymorphism.The result showed the average heterozygosity(H) of these eight blood protein (enzyme) sites in Mongolian horse,Sanhe horse and Thoroughbred horse were 0.3782,0.3956,0.2571,respectively.The cluster analysis suggested that the relationship between Mongolian horse and Sanhe horse was closer,however,the relationship between these two types of horses and Thoroughbred horse were relatively farther.
Key words:
Horse,
Blood proteins,
Genetic polymorphism,
Cluster analysis
中图分类号:
杨虹, 孟克, 王烨辉, 芒来. 不同品种马血液蛋白(酶)遗传多态性及聚类分析[J]. 华北农学报, 2009, 24(2): 103-106. doi: 10.7668/hbnxb.2009.02.022.
YANG Hong, MENG Ke, WANG Ye-hui, Dugarjaviin Manglai. Genetic Polymorphism and Cluster Analysis on Blood Protein (Enzyme)of Different Breeds of Horses[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(2): 103-106. doi: 10.7668/hbnxb.2009.02.022.