摘要： 为了探讨水杨酸能否提高水稻对镉胁迫的抗性,在1/2浓度的木村B营养液内培养水稻幼苗后,添加CdCl2达500μmol/L,然后加入外源SA使最终浓度达300μmol/L。试验结果表明,镉胁迫使水稻叶片SOD,CAT,POD活性降低,叶绿素含量下降,细胞膜透性增加,MDA含量增多。而外源的SA加入后,能使镉胁迫条件下水稻叶片SOD,CAT,POD活性降低较少,活性氧的生成速率降低,使H₂O₂含量和MDA含量降低,在一定程度上减轻镉对叶绿素的破坏和提高叶片的光合能力。说明水杨酸对受镉胁迫的水稻抗氧化酶活性产生明显的影响,能提高水稻幼苗对镉胁迫的抗性。

关键词: 水稻幼苗, 水杨酸, 镉胁迫, 活性氧

Abstract: To investigate whether tolerance to cadmium (Cd) stress of rice seedlings could be enhanced or not by exogenous salicylic acid (SA), rice seedlings were grown in half concentration of Kimura Solution B, CdCl2.500 mol/ L and 300mol/ L SA(SA1), or 500mol/ L SA(SA2) was added to the solution, and the free Salicylic acid as control (CK). Under Cd stress condition, the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), chloro??phyll content and the photosynthesis rate of rice leaves were greatly decreased, andcontent of MDA in rice leaves weregreatly increased. But after SA was added to the nutrition solution with Cd, the act ivities of SOD, CAT and POD in riceleaves were greater than that with Cd stress, and the rate of reactive oxygen production, the content of H₂O₂ and MDA was decreased, the content of chlorophyll greatly decreased. All these results indicated that the tolerance to cadmium stress of rice seedlings could be increased by exogenous SA through its effects on the activities of antioxidant enzyme.

Key words: Rice seedling, Salicylic acid, Cadmium stress, React ive oxygen

中图分类号: 

• S511.01