大白菜和黑芥种间杂种的获得及鉴定

doi:10.7668/hbnxb.2008.04.025

华北农学报 ›› 2008, Vol. 23 ›› Issue (4): 105-109. doi: 10.7668/hbnxb.2008.04.025

所属专题： 白菜；

大白菜和黑芥种间杂种的获得及鉴定

温贵聚^1,2, 赵泓², 郭仰东¹, 刘凡²

1. 中国农业大学农学与生物技术学院,北京 100094;
2. 北京市农林科学院蔬菜研究中心,北京 100097

收稿日期:2008-02-22 出版日期:2008-08-28
通讯作者: 刘凡(1965-),女,四川成都人,研究员,主要从事蔬菜生物技术育种研究工作
作者简介:温贵聚(1982-),男,山东潍坊人,在读硕士,主要从事蔬菜育种研究工作
基金资助:
北京市自然科学基金(5073045);国家自然科学基金(30771206)

Production and Identification of Interspecific Hybrids between Brassica campestris L.ssp.pekinensis and B.nigra

WEN Gui-ju^1,2, ZHAO Hong², GUO Yang-dong¹, LIU Fan²

1. College of Agronomy and Biotechnology,China Agricultural University,Beijing 100094,China;
2. Beijing Vegetable Research Center,Beijing 100097,China

Received:2008-02-22 Published:2008-08-28

摘要/Abstract

摘要： 为丰富大白菜的抗病基因类型,特别是培育根肿病抗性种质,以品质优良的大白菜(Brassica campestrisL.pekinensis)自交系为母本,具有黑腐、根肿病抗性的野生黑芥(B.nigra)为父本,通过种间杂交,获得了13株杂种植株。利用形态学、细胞学和分子标记3种方法对杂种进行了鉴定,并分析了杂种的花粉活力及其育性。结果表明:杂种表型介于白菜和黑芥双亲之间,SRAP分子标记结果的聚类分析表明杂种在DNA水平上更趋向于白菜母本。F₁雄蕊发育不好,花粉育性低,13株杂种中,仅获得杂种H₄的回交后代。杂种H₄的部分细胞染色体数目超过18条,约48%的细胞染色体数目为26条,超过了预期杂种染色体的数目。不育F₁植株染色体数目等于和少于18条,结果提示杂种育性和细胞染色体数目有一定关系,染色体数目的增加提高了杂种的育性。

关键词: 种间杂交, 大白菜, 黑芥, 杂种鉴定

Abstract: In order to enrich the germplasm resources of B. campestris, crosses between Brassica. campestris L. pekinensis and B. nigra were made and thirteen hybrids were produced. Three methods including morphology,cytology and SRAP (sequence-related amplified polymorphism) molecular makers were used to verify the hybrids 1 The pollen stain-ability and fertility of the hybrid plants were also analysed.Morphologically the hybrids showed an intermediate phenotype while the dendrogramon SRAP data indicated that the hybrids were homophylic to the female parent. The pollen stainabil-ity of the hybrids was low,among which only one(H4)could produce seeds after backcrossed to the B campestris. Some of the cells in the hybrid H4 had 18 chromosomes while 48 percent of cells contained 26 chromosomes;The other twelve sterility hybrids had 18 or less than 18 chromosomes. Results indicated that the fertility of the hybrids was related to the chromosome number and the increased number of chromosomes could enhance fertility.

Key words: Interspecific hybridization, Brassica. campestris L. ssp. pekinensis, B. nigra, Identification of hybrids

中图分类号:

S634.103.6

温贵聚, 赵泓, 郭仰东, 刘凡. 大白菜和黑芥种间杂种的获得及鉴定[J]. 华北农学报, 2008, 23(4): 105-109. doi: 10.7668/hbnxb.2008.04.025.

WEN Gui-ju, ZHAO Hong, GUO Yang-dong, LIU Fan. Production and Identification of Interspecific Hybrids between Brassica campestris L.ssp.pekinensis and B.nigra[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 105-109. doi: 10.7668/hbnxb.2008.04.025.

http://www.hbnxb.net/CN/Y2008/V23/I4/105

参考文献

[1] 张德双,徐家炳,曹鸣庆.大白菜转育新型甘蓝型油菜细胞质雄性不育系的研究[J].华北农学报,2002,17(1):60-63.
[2] 江莹芬.埃塞俄比亚芥和白菜型油菜远缘杂种F1的鉴定[J].中国油料作物学报,2007,29(2):103-106.
[3] 张德双,张凤兰,方智远.大白菜细胞质雄性不育的分子鉴定及序列分析[J].华北学报,2007,22(6):53-59.
[4] 司军,李成琼,肖崇刚.十字花科植物根肿病及抗根肿病育种研究进展[J].西南农业学报,2002,15(2):69-72.
[5] 孙保亚,沈向群,郭海峰,等.十字花科植物根肿病及抗根肿病育种研究进展[J].中国蔬菜,2005(4):34-37.
[6] sjodin C,Glimefius K.Evaluation of resistance tO Plasmodiophora brassicae,Aternaria and Phoma in Brassicaceae[J].Acta Hort,1989,459(1):363-369.
[7] Piao Z Y,Deng Y Q,Lim Y P,et al.SCAR and CAPS mapping of CRb,a gene conferring resistance to Plasrnodiophora brassicae in Chinese cabbage[J].Theor Appl Genet,2004,108(3):1 458-1 465.
[8] Puhler A,Arlat M,Pecker A,et al.What can bacterial genome research teach tls about bacteria-plant interactioas[J].Current Opinion in Plant Biology,2004,134:137-147.
[9] Rimmer S R,vail den Berg C G J.Resistance of oilseed Brassica spp.to blackleg caused by Leptosphaeria maculans[J].Can J Plant Pathol,1992,14(1):56-66.
[10] Sjoin C,Glimelius K.Transfer of resistance against phoma lingam to Brassica napus by asymmetric somatic hybridization combined with toxin selection[J].Theor Appl Genet,1989,78(2):513-520.
[11] Chevre A M,Barret P,Tanguy X,et al.Chracterization of Brassiea nigra chromosomes and of blackleg resistance in B.napus-B.nigra addition lines[J].Plant Breeding,1996,115:113-118.
[12] Dixefius C.Inheritance of the resistance to Leptosphaeria moaculans of Brassica.nigra and B.juncea in near-isogenic lines of B.napas[J].Plant Breeding,1999,118(2):151-161
[13] Saal B,Struse D.RGA and RAPD-derived SCAR markers for a Brassica B-genome introgression conferring resistance to blackleg in oilseed rape[J].Theor Appl Genet,2005,111(1):281-290.
[14] Chevre A M,Eber F,Brace J.Identification of the different Brassica nigra chromosomes from both sets of B.oleracea B.nigra and B.napus-B.nigra addition lines with a special emphasis on chromosonlle transmission and self-incompatiblity[J].Theor Appl Genet,1997,94:603-611.
[15] Pearson O H.Cytoplasmically inherited male sterility characters and flavor components from the species cross Brassica nigra(L.)Koch×B.oleracea L[J].J Amer Soc Hortic Sci,1972,97:397-402.
[16] 张丽.花椰菜和黑芥非对称体细胞杂种的获得和鉴定[D].北京:首都师范大学,2007.
[17] Narasimhulu S B,Kirti P B,Prakash Shyam.Resynthesis of Brassica carinata by protoplast fusion and recovery ofa novel cytoplasmic hybrid[J].Plant Cell Reports,1992,11(2):428-432.
[18] 李再云,Ceccarelli M.高频率产生芸薹属非整倍体和纯合植株及基因组原位杂交分析[J].中国科学,2002,32(3):218-224.
[19] 魏文辉,张苏锋,李均.白芥×甘蓝F1代及BC2代单体异附加系的GISH分析[J].科学通报,2006,51(21):2490-2514.

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大白菜和黑芥种间杂种的获得及鉴定

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