摘要： 为了研究大葱对紫斑病的抗性,进行了苗期抗性鉴定。以抗病性差异很大的2个品种为材料,分析了感病前后叶绿素、类胡萝卜素、过氧化物酶、丙二醛、脯氨酸、可溶性糖、可溶性蛋白质、游离氨基酸含量变化。结果显示,不论是抗病材料还是感病材料,发病后叶绿素含量和类胡萝卜素含量都呈下降趋势,但感病材料下降的幅度大。发病前后,抗病材料过氧化物酶活性明显高于感病材料,发病后2材料的过氧化物酶活性均大幅度提高,但抗病材料增幅大于感病材料。抗病材料未发病时MDA含量差异很小,发病后MDA均增加,但感病材料MDA含量明显高于抗病材料。抗病材料脯氨酸的自然含量高于感病材料,发病后两类材料脯氨酸含量都上升,抗病材料明显高于感病材料。抗病材料可溶性蛋白质的自然含量差异不大,发病后抗病材料蛋白质含量呈下降趋势,而感病材料则略有增加。大葱受紫斑病侵染后,无论抗病材料还是感病材料,可溶性糖含量都呈下降的趋势,但抗病材料下降的幅度小于感病材料。感、抗病材料游离氨基酸的自然含量高于感病材料,发病后感病材料游离氨基酸含量有所增加,而抗病材料的含量下降。
关键词:
大葱,
紫斑病,
生理特性
Abstract: In order to study resistance of welsh onion to alternaria leaf spot,evaluation of resistance of welsh onion seedling was done.Also,two varieties with different resistance to welsh onion alternaria leaf spot were used to study changes of chlorophyll content,carotenoid content,POD activity,MDA content,proline content,soluble protein content,soluble sugar content and free amino acid content.The results showed that the chlorophyll content and carotenoid content dropped on both varieties.But the susceptible cultivar dropped more than the resistant one.The POD activity of the resistant cultivar was higher than the susceptible one,and increased more than the latter.The MDA contents between two cultivars were little difference before infection.The susceptible cultivar increased more than the resistant one after infection.The proline content of the resistant cultivar was higher than the susceptible one,and increased more than the latter after infection.The soluble protein contents between two cultivars were little difference before infection.The susceptible cultivar increased a little,and the resistant one dropped after infection.The soluble sugar content of both cultivars dropped after infection,but the susceptible cultivar dropped more than the resistant one.The free amino acid content of the resistant cultivar was higher than the susceptible one,and the susceptible cultivar increased a little,and the resistant one decreased after infection.
Key words:
Welsh onion,
Altemaria leaf spot,
Physiological characteristics
中图分类号:
梁艳荣, 胡晓红, 张颖力, 姜伟, 陈春梅, 张少英. 大葱抗感紫斑病品种生理特性研究[J]. 华北农学报, 2008, 23(2): 169-172. doi: 10.7668/hbnxb.2008.02.038.
LIANG Yan-rong, HU Xiao-hong, ZHANG Ying-li, JIANG Wei, CHEN Chun-mei, ZHANG Shao-ying. Physiologic Differences between Resistant and Susceptible Cultivars Infected by Welsh Onion Alternaria Leaf Spot[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(2): 169-172. doi: 10.7668/hbnxb.2008.02.038.