摘要： 对郎家园小枣进行消毒、组培苗增殖以及促进生根试验,结果表明:水培休眠期一年生枝的幼芽作为外植体材料,升汞处理5~6 min,其污染率仅为5%;继代培养30 d期间,MS+AC 0.3 g/L+BA 1.5+KT 0.5+CM 50 mL/L增殖培养基处理的组培苗生长最快,其生长高度为(4.30±0.46)cm,且生长健壮,叶色正常;0.5 MS+IBA 1.5的生根培养基在产生愈伤组织、产生根系数量以及根系生长长度三个方面均表现出良好的效果。处理21 d后的愈伤组织直径达(0.45±0.04)cm;根系数量多达(5.0±0.82)条。

关键词: 郎家园小枣, 组培, 增殖, 生根, 培养基, 外植体

Abstract: For quickly establishing the virus-free lines of Ziziphus jujube Mill cv.′Langjiayuan′,the different explants and surface sterile methods,the effects of different media on the proliferation and the rooting were studied.The results showed that the explants from the water-culturing one-year young shoot tips in dormancy and the surface-sterilization with hydrargyrum solution for 5-6 min showed the best results,which contaminating rate was only 5%.During the 30 d subculture,the plantlets proliferated the best on the medium of MS+ AC 0.3 g/L+BA 1.5+KT 0.5+CM 50 mL/L.The plantlet growed(4.30±0.46)cm height and strong with normal leaf color.The rooting medium of 0.5 MS +IBA 1.5 performed good efficiency on callus-inducing,rooting and root length.The diameter of the calli reached to(0.45±0.04) cm and the root number was more than(5.0±0.82) after 3-week culture.The length of the new roots was(4.4±0.33) cm after 6-week culture.Therefore,the explants from the 1-year′s shoot tips in dormancy after water-culture,the surface sterile method with hydrargyrum for 56 min were proved as the best choice.The better proliferation medium was MS+ AC 0.3 g/L supplemented with BA 1.5,KT 0.5 and CM 50 mL/L for the sub-culture and the rooting medium was 0.5 MS +IBA 1.5 in Ziziphus jujube Mill cv.′Langjiayuan′ respectively.

Key words: Ziziphus jujube Mill cv .′Langjiayuan′, Tissue culture, Proliferation, Rooting, Culture medium, Explant

中图分类号: 

• S665.1