摘要： 为深入探究miR-181a-5p在鹅颗粒细胞中可能的功能及其作用机制奠定基础。以多个网络数据库资源为基础，通过生物信息学的方法预测可能的靶基因及其信号通路，然后利用qPCR检测miR-181a-5p及其靶基因在不同阶段颗粒层中的表达水平。结果显示，miR-181a-5p在脊椎动物中高度保守；靶基因的GO和KEGG分析发现，miR-181a-5p主要参与调控细胞的增殖、分化、凋亡等过程，多数靶基因主要富集到FOXO、MAPK、PI3K-Akt等信号通路；根据靶基因互作网络图，筛选出10个核心靶基因VEGFA、MAPK1、FOS、KRAS、HRAS、SIRT1、BCL2、ESR1、PTEN和CDKN1A，其中多数靶基因均与细胞增殖凋亡相关。结果表明，miR-181a-5p在2~4 mm表达量最高，但在4~6 mm急剧下降，之后在4~6 mm、8~10 mm、F5这3个阶段均呈现显著上升趋势。miR-181a-5p可能通过靶向调控SIRT1、BCL2、CDKN1B、PTEN等基因，参与FOXO、MAPK、PI3K-Akt等信号通路来调控颗粒细胞的增殖凋亡过程。

关键词: miR-181a-5p, GO和KEGG分析, 表达分析, 颗粒细胞, 增殖, 凋亡

Abstract: To lay a foundation for further explore of the possible function and mechanism of miR-181a-5p in geese granulosa cell. Based on various network database, we predicted the potential targets and associated signaling pathways of miR-181a-5p through bioinformatics methods. Then the expression of miR-181a-5p and its targets in different stages granular layer were detected by qPCR.The results showed that miR-181a-5p was highly conserved in vertebrates. The GO and KEGG analysis revealed that miR-181a-5p mainly involved in the regulation of cell proliferation, differentiation and apoptosis. Most of target genes were mainly enriched in FOXO, MAPK, and PI3K-Akt signaling pathways. According to the interaction network of target genes, 10 core target genes (VEGFA, MAPK1, FOS, KRAS, HRAS, SIRT1, BCL2, ESR1, PTEN, CDKN1A) were screened out and most of these targets were associated with cell proliferation and apoptosis. The levels of miR-181a-5p was the highest in 2-4 mm follicles,but declined sharply in the 4-6 mm and showed an upward trend in the 4-6 mm, 8-10 mm, and F5 follicles.miR-181a-5p might regulate the SIRT1, BCL2, CDKN1B, PTEN, etc, then participate in FOXO, MAPK, PI3K-Akt signaling pathway to regulate the proliferation and apoptosis of granulosa cells.

Key words: miR-181a-5p, GO and KEGG analysis, Expression analysis, Granulosa cells, Proliferation, Apoptosis

中图分类号: 

• S835