摘要： 利用已报道的8个与水稻条纹叶枯病抗性基因Stv-bi连锁的标记,即SCAR标记ST-10;STS标记H11-8、H11-12;InDel标记M68.4和M79.1以及SSR标记Rm21-8、Rm21-15、H21,结合田间抗性鉴定,对24份水稻品种(系)(包括2份抗、感对照)以及2个抗感组合的F2分离世代进行抗病性分析、筛选和评价,旨在有效利用抗病资源选育抗病新品种,并对这8个分子标记的有效性和选择效率进行验证比较,筛选出高效的辅助选择分子标记。结果表明,仅有SCAR标记ST-10和STS标记H11-8的分子检测与田间表型鉴定的吻合率超过或接近90%,其余分子标记均低于80%,其中,3对SSR标记在抗感材料中几乎没有多态性。比较而言,SCAR标记ST-10更能有效地用于条纹叶枯病抗性材料的筛选,结合使用STS标记H11-8更能准确有效地检测Stv-bi基因的存在,进一步提高选择效率。
关键词:
水稻,
条纹叶枯病,
抗性基因,
分子检测,
SCAR,
Indel,
STS,
SSR
Abstract: In this study，we developed eight resistance-associated markers ST-10，H11-8，H11-12，M68． 4， M79． 1，Rm21-8，Rm21-15 and H21，as reported previously， to evaluate the resistance of RSD in 24 rice varieties ( including two anti-and sense-varieties) and two F2 populations obtained from a cross between different anti-and sense-varieties． To breed new RSD-resistant rice varieties，we also compared the effectiveness and selection efficiency of these eight markers，and screened the most efficient markers to assist breeding． Qur results showed that: only the consistency rate of markers ST-10 and H11-8 between molecular detection and field phenotypic identification was greater than or close to 90%; The rest of the molecular markers were lower than 80%; Of these， three SSR markers were almost no polymorphism． Compared to STS marker H11-8，SCAR marker ST-10 was more effective for screening RSD resistant varieties． The combined use of these two markers was more accurate and efficient to detect the Stv-bi gene and to further improve the selection efficiency．
Key words:
Rice,
Rice stripe disease,
Resistance gene,
Molecular detection,
SCAR,
Indel,
STS,
SSR
中图分类号:
姚姝, 张亚东, 朱镇, 陈涛, 赵庆勇, 周丽慧, 于新, 王才林. 8个水稻抗条纹叶枯病分子标记有效性的验证及评价[J]. 华北农学报, 2012, 27(6): 146-151. doi: 10.3969/j.issn.1000-7091.2012.06.029.
YAO Shu, ZHANG Ya-dong, ZHU Zhen, CHEN Tao, ZHAO Qing-yong, ZHOU Li-hui, YU Xin, WANG Cai-lin. Evaluation and Validation of Eight Molecular Markers Associated with Rice Stripe Resistance in Rice ( Oryza sativa)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 146-151. doi: 10.3969/j.issn.1000-7091.2012.06.029.