鸡脐带间充质干细胞向胰岛β样细胞诱导分化的研究

doi:10.3969/j.issn.1000-7091.2012.04.023

华北农学报 ›› 2012, Vol. 27 ›› Issue (4): 123-129. doi: 10.3969/j.issn.1000-7091.2012.04.023

所属专题： 畜牧；

鸡脐带间充质干细胞向胰岛β样细胞诱导分化的研究

武爽, 关伟军, 马月辉

中国农业科学院北京畜牧兽医研究所, 北京100193

出版日期:2012-08-28
通讯作者: 关伟军（1966-），男，黑龙江人，教授，博士生导师，主要从事动物遗传资源研究。马月辉（1964-），男，吉林人，研究员，博士生导师，主要从事动物遗传资源研究。
作者简介:武爽（1986-），女，黑龙江哈尔滨人，在读硕士，主要从事动物遗传资源研究。
基金资助:
转基因生物新品种培育科技重大专项（2011ZX08009-003-006;2011ZX08012-002-06）;基本科研业务项目（2011cj-9）;国家家养动物种质资源平台项目（2012）

Differentiation of Umbilical Cord Mesenchymal Stem Cells （UCMSCs） Derived from Chicken into Isletβ-cells in vitro

WU Shuang, GUAN Wei-jun, MA Yue-hui

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Published:2012-08-28

摘要/Abstract

摘要： 为了建立鸡脐带间充质于细胞（Umbilical cord mesenchymal stem cells，UCMSCs）体外培养体系并对其进行生物学特性研究。取11—15日龄鸡胚，脐带分离获得UCMSCs，在L-DMEM培养基中培养，观察其形态。通过传代、生长曲线和周期分析其增殖情况，并研究体外诱导分化潜能。结果表明，鸡UCMSCs可在体外扩增培养，目前传代至P30，表达CD29、CD44和CD71，并具有向胰岛β样细胞诱导分化的潜能。鸡脐带中可以分离出UCMSCs，能够在体外培养，并可诱导分化为胰岛口样细胞。

关键词: 鸡, 脐带间充质干细胞, 分离培养, 鉴定, 诱导分化

Abstract: The aim was to study on the biological characteristics of chicken UCMSCs by isolating culture in vitro. UCMSCs was isolated from umbilical cord of 11 -15 day-old chicken embryos. The suitable culture medium for UCMSCs was L-DMEM. Then the morphology and the growing proliferation condition and the differentiation po- tential in vitro of the chicken UCMSCs were observed. The results showed that Chicken UCMSCs can be cultured in vitro and have been passed to 30 generation. The UCMSCs expressed CD29,CD44 and CDT1,results displayed that the UCMSCs had the differentiation potential to isletβ-cells. UCMSCs from chicken can proliferate rapidly in vitro and be induced to isletβ-cells.

Key words: Chicken, UCMSCs, Isolation and in vitro culture Identification, Differentiation potential

中图分类号:

武爽, 关伟军, 马月辉. 鸡脐带间充质干细胞向胰岛β样细胞诱导分化的研究[J]. 华北农学报, 2012, 27(4): 123-129. doi: 10.3969/j.issn.1000-7091.2012.04.023.

WU Shuang, GUAN Wei-jun, MA Yue-hui. Differentiation of Umbilical Cord Mesenchymal Stem Cells （UCMSCs） Derived from Chicken into Isletβ-cells in vitro[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 123-129. doi: 10.3969/j.issn.1000-7091.2012.04.023.

http://www.hbnxb.net/CN/Y2012/V27/I4/123

参考文献

[1] Karahuseyinoglu S, Kocaefe C, Balci D, et al. Functional structure of adipocytes differentiated from human umbili- cal cord stroma-derived stern cells[J]. Stern Cells,2008, 26(3) :682 -691.

[2] Karahuseyinoglu S, Cinar O, Kilic E,et al. Biology of stem cells in human umbilical cord stroma:In situ and in vitro surveys [J]. Stem Cells, 2007,25 : 319 - 331.

[3] Lumelsky N, Blondel O, Laeng P, et al. Differentiation of embryonic stem cells to insulin-secreting structures simi- lar to pancreatic islets [J]. Science, 2001,292 ( 5520 ) : 1389 - 1394.

[4] Vaca P, Mart n F, Vegara-Meseguer J M, et al. Induction of differentiation of embryonic stem cells into insulin-se- creting ceils by fetal soluble factors [J]. Stem Cells, 2006,24 ( 2 ) : 258 - 265.

[5] Segev H, Fishman B, Ziskind A, et al. Differentiation of human embryonic stem cells into insulin-producing clus- ters[J]. Stem Cells,2004,22(3 ) :265 - 274.

[6] Moriscot C, Fraipont F D, Richard M J,et al. Human bone marrow mesenchymal stem cells can express insulin and key transcription factors of the endocrine pancreas devel- opmental pathway upon genetic and/or microenvironmen- tal manipulation in vitro [J]. Stem Cells, 2005,23 ( 4 ) : 594 - 603.

[7] Seaberg R M,Smukler S R,Kieffer T J,et al. Clonal iden- tification of muhipotent precursors from adult mouse pan- creas that generate neural and pancreatic lineages [J]. Nat Biotech,2004,22 : 1115 - 1124.

[8] Tai M H,Karl O L,Burra V M,et al. Characterization of gap junctional intercellular communication in immortalized human pancreatic ductal epithelial cells with stem cell characteristics [J]. Pancreas, 2003,26 ( 1 ) : 18 - 26.

[9] Linning, Katrina D, Tai M H, et al. Redox-mediated en- richment of self-renewing adult human pancreatic cells that possess endocrine differentiation [J]. Potential Pan- creas,2004,29(3 ) :64 - 67.

[10] Yang L J, Li S W, Hatch H, et al. In vitro transdifferenti- ation of adult hepatic stem cells into pancreatic endo- crine hormone-producing cells [J]. Proc Natl Acad Sci USA,2002,99:8078 - 8083.

[11] Hori Y C, Gu X Y, Xie X D, et al. Differentiation of in- sulin-producing cells from human neural progenitor ceils [J]. PLoS Med,2005,2:347 -356.

[12] Ende N,Chen R F, Reddi Alluru S. Effect of human um- bilical cord blood cells on glycemia and insulitis in type 1 diabetic mice [J]. Biochem Biophys Res Commun, 2004,325:665 - 669.

[13] Ende N, Chen R F, Reddi A S. Transplantation of human umbilical cord blood cells improves glycemia and glo- merular hypertrophy in type 2 diabetic mice [J]. Bio- chem Biophys. Res Commun,2004,321:168 - 171.

[14] Petsa A, Gargani S, Felesakis A,et al. Effectiveness of protocol for the isolation of Wharton's Jelly stem cells in large-scale applications[J]. In Vitro Cell Dev Biol -Ani- mal,2009,45:573 - 576.

[15] Chao K C,Chao K F,Fu Y S,et al. Islet-like clusters de- rived from mesenchymal stem cells in Wharton's Jelly of the human umbilical cord for transplantation to control type 1 diabetes[J]. PLoS One,2008 (1) :1451.

[16] Takahash I T, Kalka C, Masuda H,et al. Ischemia and cytokine induced mobiliazatio of bone marrow derived endothelial progeitor cells for neovasculation [J]. Nat Med, 1999,8:434 -438.

[17] Zulewski H,Abraham E J,Gerlach M J,et al. Multipo- tential nestin-positive stem cells isolated from adult pan- creatic endocrine, exocrine and hepatic phenotypes [J]. Diabetes,2001,50:521 - 533.

[18] Otonkoski T,Beattie G M,Mally M I,et al. Nicotinamide is a potent inducer of endocrine differentiation in cul- tured human fetal pancreatic cells [J]. J Clin Invest, 1993.92 : 1459 - 1466.

[19] Roche E,Buteau J,Aniento I,et al. Induction by glucose of genes coding for glycolytic enzymes in a pancreatic beta-cell line(INS-1 ) [J]. J Boil Chem,1997,272(5) : 3091 - 3098.

[20] Ramiya V K, Maraist M, Arfors E,et al. Reversal of insu- lin dependent diabetes using islets generated in vitro from pancreatic stem ceils [J]. Nat Med, 2000,6 ( 3 ) : 278 - 282.

选择文件类型/文献管理软件名称

选择包含的内容

鸡脐带间充质干细胞向胰岛β样细胞诱导分化的研究

Differentiation of Umbilical Cord Mesenchymal Stem Cells （UCMSCs） Derived from Chicken into Isletβ-cells in vitro

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	肖继兵, 刘志, 辛宗绪, 陈国秋, 吴宏生. 基于GGE双标图的谷子种质资源耐旱性鉴定[J]. 华北农学报, 2022, 37(5): 87-96.
[2]	宿放, 韩佃刚, 叶玲玲, 张冲, 尹尚莲, 罗倩敏, 董仙兰, 李瑶瑶, 李凌枫, 艾军, 信吉阁. COVID-19病毒N基因在昆虫细胞内的表达[J]. 华北农学报, 2022, 37(5): 174-180.
[3]	凌晨, 赵洪, 颜军, 马莹雪, 冯艳芳, 张靖立, 李波, 邓超, 徐振江. 基于SSR标记的莴苣品种鉴定体系的建立及应用[J]. 华北农学报, 2022, 37(4): 34-44.
[4]	张辉, 王良, 付增娟, 李晓东, 赵尚敏, 鄂圆圆, 郑文哲, 张自强, 张必周, 张惠忠. VNTR特异性引物在甜菜育性鉴定中的应用[J]. 华北农学报, 2022, 37(2): 35-41.
[5]	张键, 王娜, 刘志达, 包婷婷, 张之为, 云晓鹏, 石必显, 张恒, 陈贵红, 陈燕芳, 赵君. 向日葵列当枯斑病病原菌的分离鉴定及致病力分化[J]. 华北农学报, 2022, 37(1): 172-180.
[6]	宋祥军, 宋自超, 沈啸, 陈哲, 蒋胡艳, 侯曼曼, 邵颖, 涂健, 祁克宗. 禽致病性大肠杆菌Hcp2b对雏鸡脾脏mRNA表达谱的影响[J]. 华北农学报, 2022, 37(1): 232-238.
[7]	叶德友, 漆永红, 张化生. 角瓜与南方根结线虫非亲和互作相关MicroRNAs的鉴定[J]. 华北农学报, 2021, 36(S1): 333-340.
[8]	杨金睿, 王文倩, 王玥, 姚遥, 吉泽, 李俊逸, 陈斌, 肖关丽. 云南省4个主栽马铃薯品种叶片内生细菌多样性研究[J]. 华北农学报, 2021, 36(6): 178-188.
[9]	刘丹, 梁丹, 王从磊, 时晓伟, 许庆芬, 高杨, 候康波, 张欣, 冯刚, 王建贺. 小麦籽粒不同发育阶段醇溶蛋白表达谱分析及在种子纯度鉴定中的应用[J]. 华北农学报, 2021, 36(2): 68-73.
[10]	岳润清, 刘璐, 铁双贵, 徐心志, 陈娜娜. *转Cry1Ab-t基因玉米YA108的获得及其抗虫性鉴定*[J]. 华北农学报, 2021, 36(2): 212-218.
[11]	刘晨, 王祯, 朱先约, 杨瑞, 倪博立. 苏云金芽孢杆菌的分离鉴定及伴孢晶体蛋白生物毒力效果分析[J]. 华北农学报, 2020, 35(S1): 355-362.
[12]	张康, 张凯, 王磊, 崔东安, 张景艳, 闫遵祥, 马雪青, 薛欢, 李建喜. 一株牛病毒性腹泻病毒BVDV-GSLY的分离鉴定[J]. 华北农学报, 2020, 35(S1): 378-384.
[13]	李佳骏, 李建臻, 李万强, 王振华, 王君锐, 肖丹, 潘康成, 杨苗. SSH文库技术结合DGGE技术筛选饲喂蜡样芽孢杆菌肉鸡脾脏免疫差异基因[J]. 华北农学报, 2020, 35(S1): 392-398.
[14]	王海威, 王珍, 李星, 李静, 解华东, 王启贵. 不同饲养方式、饲养时间对杂交优质肉鸡肉品质的影响[J]. 华北农学报, 2020, 35(S1): 399-408.
[15]	吕亮杰, 陈希勇, 张文英, 赵爱菊, 孙丽静, 张颖君, 刘玉平, 王莉梅, 李子千, 李辉. EMS诱变抗旱小麦冀麦418的突变体筛选与鉴定[J]. 华北农学报, 2020, 35(S1): 47-55.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

鸡脐带间充质干细胞向胰岛β样细胞诱导分化的研究

Differentiation of Umbilical Cord Mesenchymal Stem Cells （UCMSCs） Derived from Chicken into Isletβ-cells in vitro

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价