人工异源六倍体小麦中甲基化差异的MSAP分析

doi:10.3969/j.issn.1000-7091.2012.02.015

华北农学报 ›› 2012, Vol. 27 ›› Issue (2): 77-80. doi: 10.3969/j.issn.1000-7091.2012.02.015

所属专题： 小麦；

人工异源六倍体小麦中甲基化差异的MSAP分析

聂利红¹, 王延召¹, 孙其信², 房卫平¹, 谢德意¹

1. 河南省农业科学院经济作物研究所, 河南郑州 450002;
2. 西北农林科技大学, 陕西杨凌 712100

收稿日期:2011-12-20 出版日期:2012-04-28
通讯作者: 孙其信(1962－), 男, 甘肃景泰人, 教授, 博士生导师, 主要从事作物遗传育种研究。
作者简介:聂利红(1979－), 女, 河南安阳人, 副研究员, 博士, 主要从事植物进化和遗传育种研究。
基金资助:
国家自然科学基金(30370878)

MSAP Analysis of DNA Methylation Alteration in Synthetic Hexaploid Wheat

NIE Li-hong¹, WANG Yan-zhao¹, SUN Qi-xin², FANG Wei-ping¹, XIE De-yi¹

1. Economic Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;
2. Northwest A&F University, Yangling 712100, China

Received:2011-12-20 Published:2012-04-28

摘要/Abstract

摘要： 利用MSAP方法分析了小麦从四倍体到六倍体进化过程中甲基化水平和甲基化遗传模式的变化。结果表明,人工异源六倍体小麦SCA/SQ(AABBDD)及其四倍体母本SCAUP(AABB)、二倍体父本粗山羊草SQ523(DD)的总甲基化水平分别为28.21%,27.53%,24.11%。37对引物检测到1 087条差异的扩增片段,对这些位点的甲基化遗传模式进行分析,结果表明,在人工异源六倍体中发生过甲基化的比例为18.95%,高于去甲基化的比例(2.58%)。这些结果揭示了小麦从四倍体到六倍体的进化过程中,通过对一些功能基因的甲基化来减轻异源多倍化造成的影响。

关键词: 人工六倍体小麦, 异源多倍化, 甲基化, 甲基化敏感扩增多态性

Abstract: DNA methylation is known to play an important role in the regulation of gene expression in higher?plants． In this study，we assessed the extent and patterns of cytosine methylation alterations in a stable synthetic hexaploid wheat and their progenitor parents using MSAP． Results showed that the levels of methylation in SCA/SQ?(AABBDD) and the parents were 28． 21%，27． 53%，24． 11%，respectively． A total of 1 087 fragments were differentially amplified from the two digests for at least one of the parental lines，about 21． 53% of loci shown changes in?methylation patterns in the allopolyploid． Among these loci，18． 95% were hypermethylated，2． 58% were demethylated． The increased methylation level in synthetic hexaploid wheat compared to its parents might mitigate the shocks?from the hybridization and duplication．

Key words: Synthetic hexaploid wheat, Allopolyploidy, DNA methylation, MSAP

中图分类号:

S512.01

聂利红, 王延召, 孙其信, 房卫平, 谢德意. 人工异源六倍体小麦中甲基化差异的MSAP分析[J]. 华北农学报, 2012, 27(2): 77-80. doi: 10.3969/j.issn.1000-7091.2012.02.015.

NIE Li-hong, WANG Yan-zhao, SUN Qi-xin, FANG Wei-ping, XIE De-yi. MSAP Analysis of DNA Methylation Alteration in Synthetic Hexaploid Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 77-80. doi: 10.3969/j.issn.1000-7091.2012.02.015.

http://www.hbnxb.net/CN/Y2012/V27/I2/77

参考文献

[1] Comai L，Tyagi A P，Winter K，et al． Phenotypic instability?and rapid gene silencing in newly formed Arabidopsis allotetraploids[J]． Plant Cell，2000，12:1551－1568．
[2] Lee H S，Chen Z J． Protein-coding genes are epigenetically regulated in Arabidopsis polyploids[J]． Proc Natl Acad?Sci USA，2001，98:6753－6758．
[3] Yaakov B，Kashkush K． Massive alterations of the methylation patterns around DNA transposons in the first four?generations of a newly formed wheat allohexaploid[J]．?Genome，2011，54(1):42－49．
[4] Xu Y，Zhong L，Wu X，et al． Rapid alterations of gene expression and cytosine methylation in newly synthesized?Brassica napus allopolyploids[J]． Planta，2009，229(3):?471－483．
[5] Zilberman D． The evolving functions of DNA methylation
[J]． Curr Opin Plant Biol，2008，11:554－559．
[6] ?Finnegan E J，Peacock W J，Dennis E S． DNA methylation，a key regulator of plant development and other processes[J]． Curr Opin Genet Dev，2000，10:217－?223．
[7] 沈显生．浅析植物多倍体现象[J]．生物学杂志，1995, 5:8－11．
[8] Xiong L Z，Xu C G，Saghai Maroof M A，et al． Patterns of?cytosine methylation in an elite rice hybrid and its parental lines，detected by a methylation-sensitive amplification?polymorphism technique[J]． Molecular and General Genetics，1999，261:439－446．
[9] Bennett M D，Leitch I J． Nuclear DNA amounts in angiosperms-583 new estimates[J]． Ann Bot，1997，80:169－?196．
[10] ?Gaeta R T，Pires J C，Iniguez-Luy F，et al． Genomic?changes in resynthesized Brassica napus and their effect?on gene expression and phenotype[J]． Plant Cell，2007, 19:3403－3417．
[11] Leitch A R，Leitch I J． Genomic plasticity and the diversity of polyploid plants[J]． Science，2008，320:481－?483．
[12] Liu B，Vega J M，Segal G，et al． Rapid genomic changes?in newly synthesized amphiploids of Triticum and Aegilops． I． Changes in low-copy non-coding DNA sequences[J]． Genome，1998，41:272－277．
[13] Liu B，Vega J M，Feldman M． Rapid genomic changes in?newly synthesized amphiploids of Triticum and Aegilops．?II． Changes in low-copy coding DNA sequences[J]． Genome，1998，41:535－542．
[14] Shaked H，Kashkush K，Ozkan H，et al． Sequence elimination and cytosine methylation are rapid and reproducible responses of the genome to wide hybridization and allopolyploidy in wheat[J]． Plant Cell，2001，13:1749－?1759．
[15] Adams K L，Percifield R，Wendel J F． Organ-specific silencing of duplicated genes in a newly synthesized cotton?allotetraploid[J]． Genetics，2004，168:2217－2226．
[16] Chaudhary B，Flagel L，Stupar R M，et al． Reciprocal?silencing，transcriptional bias and functional divergence?of homeologs in polyploid cotton (Gossypium)[J]． Genetics，2009，182(2):503－517．
[17] Buggs R J，Elliott N M，Zhang L，et al． Tissue-specific?silencing of homoeologs in natural populations of the recent allopolyploid Tragopogon mirus [J]． New Phytol, 2010，186(1):175－183．
[18] Chen Z J，Pikaard C S． Transcriptional analysis of nucleolar dominance in polypliod plants:biased expression/silencing of progenitor rRNA genes is developmentally regulated in Brassica[J]． Proc Natl Acad Sci USA，1997, 94:3442－3447．
[19] ?Matzke M A，Matzke A J． Epigenetic silencing of plant?transgenes as a consequence of diverse cellular defence?responses[J]． Cell Mol Life Sci，1998，54 (1):94－?103．
[20] Paun O，Bateman R M，Fay M F，et al． Stable epigenetic?effects impact adaptation in allopolyploid Orchids (Dactylorhiza:Orchidaceae) [J]． Mol Biol Evol，2010，27?(11):2465－2473．

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