大白菜<em>FAD8</em>基因的克隆

doi:10.3969/j.issn.1000-7091.2012.02.007

摘要/Abstract

摘要： 脂肪酸去饱和酶基因FAD8是控制亚油酸向亚麻酸转换的关键酶,对植物的膜脂不饱和度有重要影响,关系到植物的抗寒能力,在拟南芥中是低温诱导表达的。根据已发表的FAD8序列,设计简并引物,从低温处理条件下大白菜叶片cDNA中克隆到一段200 bp的中间序列,进一步利用cDNA末端快速扩增技术(Rapid Amplification of cD-NA ends,RACE),获得大白菜FAD8基因的5’和3’末端序列,经测序拼接获得1509 bp的目的序列,编码432个氨基酸。与已经报导的油菜FAD8基因氨基酸序列同源性较高,达98%。杂交结果显示,该基因是单拷贝序列。这项工作为进一步研究叶绿体脂肪酸去饱和酶奠定了一定的基础。

关键词: 大白菜, FAD8基因, 克隆

Abstract: Fatty acid desaturase is a key gene controlled the reaction of linoleic acid to linolenic acid in chloroplast，which affect the membrane fatty acids degree of unsaturation related to plant resistance to cold． A fragment of?DNA，198 bp，of FAD8 gene were cloned in cDNA from cold induced Chinese cabbage leaf depend on the degenerated primers designed by the FAD8 sequence of other plants'． Full cDNA sequence were cloned by rapid amplification of cDNA ends(RACE) PCR，The total cDNA sequeceof FAD8，coding 432 amino acids，is 1 509 bp after sequencing and jointing． Sequence alignment shows high similarity(98%)with FAD8 of Brassica napus． Southern boltting shows it's a single copy gene in Chinese cabbage genome． This work is the basis for further study on chloroplast?fatty acids desaturase．

Key words: Chinese cabbage, Gene of FAD8, Clone

中图分类号:

S642.2
Q78

刘春香, 冷传远, 刘福. 大白菜FAD8基因的克隆[J]. 华北农学报, 2012, 27(2): 35-39. doi: 10.3969/j.issn.1000-7091.2012.02.007.

LIU Chun-xiang, LENG Chuan-yuan, LIU Fu-yan. Cloning a Full cDNA Sequence of FAD8 Gene in Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 35-39. doi: 10.3969/j.issn.1000-7091.2012.02.007.

http://www.hbnxb.net/CN/Y2012/V27/I2/35

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大白菜FAD8基因的克隆

Cloning a Full cDNA Sequence of FAD8 Gene in Chinese Cabbage

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Cloning a Full cDNA Sequence of FAD8 Gene in Chinese Cabbage

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