摘要： 采用基因芯片技术，对几种常见的肠道菌进行检测和鉴定，包括检测靶基因的扩增、基因芯片的制备、杂交反应和杂交结果的检测与分析4个步骤。结果表明：应用基因芯片对涉及4个菌属的23株肠道菌在相同的条件下进行了杂交检测，得到菌属特异性的杂交图谱，从而达到对细菌进行检测鉴定的目的。制备的基因芯片能够同时检测沙门菌属、志贺菌属、葡萄球菌属、耶尔森氏菌属的细菌。
关键词:
基因芯片,
肠道菌,
肠道菌检测
Abstract: To detect the intestinal bacteria quickly and accurately, an experimental procedure was set up using the gene chip technology to detect and identify common intestinal bacteria. Target gene was amplified and hybridized with prepared gene chip. The results showed 23 strains of bacteria in pure culture belong to 4 genera were successfully discriminated under comparatively same condition and a series of specific hybridization maps corresponding to each kinds of bacteria were obtained. Salmonella spp, Shigella spp, Proteus spp, Yersinia enterocolitica, were detected and identified by the technology of gene chip.
Key words:
Gene chip,
Intestinal bacteria,
Detection of intestinal bacteria
中图分类号:
徐晓静, 文思远, 韩毅. 应用基因芯片方法检测几种肠道菌[J]. 华北农学报, 2007, 22(1): 182-183. doi: 10.3321/j.issn:1000-7091.2007.01.043.
XU Xiao-jing, WEN Si-yuan, HAN Yi. Detection and Identification of Intestinal Bacteria by Oligonucleotide Array[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(1): 182-183. doi: 10.3321/j.issn:1000-7091.2007.01.043.