植物蛋白质的亚细胞定位研究进展

doi:10.3321/j.issn:1000-7091.2006.z2.001

摘要/Abstract

摘要： 细胞是生命形式的基本组成单元，各种蛋白质按照其功能有序地分布在细胞的每个分区中。植物细胞的主要分区包括细胞膜和其他内膜系统、细胞核、细胞质以及位于其中的线粒体、叶绿体、高尔基体和内质网等各种细胞器。植物蛋白质的亚细胞定位是功能基因组学的重要内容。主要的植物蛋白质亚细胞定位技术包括：融合报告基因定位法、免疫组织化学定位法、蛋白质组学定位技术以及共分离标记酶辅助定位法，而生物信息学预测也成为亚细胞定位研究的一种重要手段。高通量蛋白质亚细胞定位技术的发展和应用，为构建定位数据库积累了数据。在模式植物拟南芥中，有亚细胞定位信息的蛋白质已经超过4 000个。

关键词: 蛋白质, 亚细胞定位, 细胞分区, 蛋白质组学, 高通量

Abstract: Cell is the fundamental unit of living organism,proteins with different functions distributed in cellular compartments,including plasmolemma,nucleus,cytoplasm and organelles like mitochondria,chloroplast,Golgi,and endoplasmic reticulum etc.Protein subcellular localization is one of the key questions for functional genomics.The techniques used for subcellular localization of plants protein include fusion reporter gene localization,immunohistochemical localization,2D combined with mass spectrometry,marker enzyme-assisted localization and bioinformatics prediction.The development and application of high-throughtput protein subcellular localization technology stimulated the establishment of protein subcellular localization database.In model plant Arabidopsis thaliana,the number of proteins with localization data is over 4000.

Key words: Protein, Subcellular localization, Cellular compartment, Proteomics, High_throughput

中图分类号:

邢浩然, 刘丽娟, 刘国振. 植物蛋白质的亚细胞定位研究进展[J]. 华北农学报, 2006, 21(S2): 1-6. doi: 10.3321/j.issn:1000-7091.2006.z2.001.

XING Hao-ran, LIU Li-juan, LIU Guo-zhen. Advancement of Protein Subcellular Localization in Plants[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(S2): 1-6. doi: 10.3321/j.issn:1000-7091.2006.z2.001.

http://www.hbnxb.net/CN/Y2006/V21/IS2/1

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