SRAP标记分析甘蓝型油菜多态性

doi:10.3321/j.issn:1000-7091.2006.01.012

华北农学报 ›› 2006, Vol. 21 ›› Issue (1): 50-54. doi: 10.3321/j.issn:1000-7091.2006.01.012

所属专题： 油料作物；

SRAP标记分析甘蓝型油菜多态性

张书芬^1,2, 傅廷栋¹, 李媛媛¹, 朱家成², 王建平², 马朝芝¹

1. 华中农业大学遗传改良国家重点实验室, 湖北武汉 430070;
2. 河南省农业科学院棉花油料作物研究所, 河南郑州 450002

收稿日期:2005-09-13 出版日期:2006-02-28
通讯作者: 马朝芝
作者简介:张书芬（1965- ），女，河南唐河人，研究员，在读博士，主要从事油菜遗传育种研究工作。
基金资助:
国家“973”项目（2001CB10807）；国家“十五”攻关项目（2001BA511B07）；863项目（2002AA207009，2001AA241102）；河南省杰出青年基金项目（0212000700）资助

Polymorphism Analysis by Sequence-related Amplified Polymorphism Markers in Brassica napus L

ZHANG Shu-fen^1,2, FU Ting-dong¹, LI Yuan-yuan¹, ZHU Jia-cheng², WANG Jian-ping², MA Chao-zhi¹

1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China,;
2. Cotton and Oil Crops Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China

Received:2005-09-13 Published:2006-02-28

摘要/Abstract

摘要： 采用SRAP标记对甘蓝型油菜品种1141B、垦C₁、32B和32C的多态性进行了分析。每对引物组合产生13~36对比较清晰的扩增带，20对引物组合共产生419条扩增带，平均每对引物组合产生20.95条。20对引物组合共产生多态性带116条，每对引物组合产生3~10条，平均5.8条。每对引物组合产生的多态性带的比例为18.75%~38.89%，平均为28.23%。用24对引物组合对组合1141B×；垦C₁构建的F₂群体各单株多态性进行了分析，F₂群体多态性标记75条，平均为3.12条。用卡平方测验检测F₂群体各单株标记基因型频率是否偏离了期望比例1：2：1（共显性标记）和3：1（显性标记）的分离规律，结果表明，仅有1个SRAP标记偏离了1：2：1分离规律，仅占1.33%。试验结果表明，SRAP标记适于进行油菜品种的多态性分析和图谱构建，是一种经济、有效的分子标记。

关键词: 甘蓝型油菜, 多态性, SRAP标记

Abstract: Polymorphism among 1141B,KenC₁,32B and 32C were analyzed with SRAP(sequence-related amplified polymorphism) markers.There were 13-36 clear polymorphic combination bands per primer,and the average was 5.8.About 419 amplified fragments and 116 polymorphic fragments were detected 20 SRAP primer combinations.The percentage of polymorphic fragments was 28.23%.The number of amplified fragments and polymorphic fragments per primer combination were 20.95 and 5.8,respectively.The polymorphism analyzed by F₂ individual of 1141B Ken C₁ with 24 primer combinations showed that there were 75 polymorphic bands,the average was 3.12.The χ² test for allelic genotype of all loci indicated that 1.33%of the SRAP markers distorted from the expectation 3:1 ratio(dominance markers) and 1:2:1(co-dominance markers).It seems that SRAP markers was a high efficiency marker system in the paper.It showed that SRAP were an effective,cheap and dependable marker for polymorphism analysis and linkage map construction in B.napus L.

Key words: B.napus L., Polymorphim, SRAP marker

中图分类号:

S565

张书芬, 傅廷栋, 李媛媛, 朱家成, 王建平, 马朝芝. SRAP标记分析甘蓝型油菜多态性[J]. 华北农学报, 2006, 21(1): 50-54. doi: 10.3321/j.issn:1000-7091.2006.01.012.

ZHANG Shu-fen, FU Ting-dong, LI Yuan-yuan, ZHU Jia-cheng, WANG Jian-ping, MA Chao-zhi. Polymorphism Analysis by Sequence-related Amplified Polymorphism Markers in Brassica napus L[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(1): 50-54. doi: 10.3321/j.issn:1000-7091.2006.01.012.

http://www.hbnxb.net/CN/Y2006/V21/I1/50

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