雄激素通过Fas途径诱导小鼠骨髓巨噬细胞凋亡

doi:10.3321/j.issn:1000-7091.2005.04.003

华北农学报 ›› 2005, Vol. 20 ›› Issue (4): 8-11. doi: 10.3321/j.issn:1000-7091.2005.04.003

雄激素通过Fas途径诱导小鼠骨髓巨噬细胞凋亡

程瑜, 张华屏, 乔中东

上海交通大学农业与生物学院, 上海 201101

收稿日期:2005-04-15 出版日期:2005-08-28
通讯作者: 乔中东
作者简介:程瑜（1977- ），女，山西太原人，在读硕士，主要从事雄激素诱导小鼠骨髓巨噬细胞凋亡的信号转导途径研究。

Fas Pathway in Apoptosis of BMM Induced by Testosterone

CHENG Yu, ZHANG Hua-ping, QIAO Zhong-dong

College of Agricultural and Biological, Shanghai Jiaotong University, Shanghai 201101, China

Received:2005-04-15 Published:2005-08-28

摘要/Abstract

摘要： 通过雄激素诱导小鼠骨髓巨噬细胞凋亡以及对Fas信号传导通路的研究来探讨雄激素对免疫系统抑制作用的机制。采用不同浓度（1~10nmol/L）的雄激素处理小鼠骨髓巨噬细胞24 h后，先用DNA片段电泳法和Annexin-V/PI双染后流式细胞仪检测不同处理组的细胞凋亡的情况；再用RT-PCR半定量法观察雄激素对Fas途径中一些基因表达的影响。发现10 nmol/L雄激素处理的巨噬细胞凋亡率31.7%比对照组7.4%显著升高，并伴随Fas途径中相关基因（Fas，FasL，FADD，caspase-8和caspase-3）转录水平的升高。雄激素可能是通过Fas途径来诱导小鼠骨髓巨噬细胞发生凋亡，从而发挥其免疫抑制作用。

关键词: 雄激素, 凋亡, 骨髓巨噬细胞, 免疫抑制

Abstract: In this paper,mechanism involved in suppression of testosterone on immune system through investigating apoptosis of BMMs(bone marrow-derived macrophages) induced by testosterone and Fas signal pathway were discussed.After BMMs were treated with various concentration of testosterone(1-10nmol/L) for 24h,the apoptosis was analyzed by DNA fragment electrophoresisand flow cytometry with Annexin-V and PI double staining methods,and the effect of testosteroneon expression of some genes involved in Fas pathway was estimated by RT-PCR semi-quantified method.BMMs treated with 10 nmol/L testosterone had significantly higher apoptosis ratio 31.7% than contrast BMMs 7.4%,accompanying with the increasing transcription of related genes in Fas pathway such as Fas,FasL,FADD,caspase-8 and caspase-3.Then testosterone might induce apoptosis of BMM through Fas pathway to exert immunosuppression.

Key words: Testosterone, Apoptosis, BMM, Immunosuppression

中图分类号:

S865.12

程瑜, 张华屏, 乔中东. 雄激素通过Fas途径诱导小鼠骨髓巨噬细胞凋亡[J]. 华北农学报, 2005, 20(4): 8-11. doi: 10.3321/j.issn:1000-7091.2005.04.003.

CHENG Yu, ZHANG Hua-ping, QIAO Zhong-dong. Fas Pathway in Apoptosis of BMM Induced by Testosterone[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(4): 8-11. doi: 10.3321/j.issn:1000-7091.2005.04.003.

http://www.hbnxb.net/CN/Y2005/V20/I4/8

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