华北农学报 ›› 2004, Vol. 19 ›› Issue (4): 55-58. doi: 10.3321/j.issn:1000-7091.2004.04.015

所属专题: 黄瓜

• 论文 • 上一篇    下一篇

黄瓜雌核发育早期过氧化物同工酶活性的变化

王晓云1, 杜胜利2, 曹翠玲1, 魏爱民2, 韩毅科2, 张桂华2   

  1. 1. 西北农林科技大学, 陕西 杨凌 712100;
    2. 天津科润黄瓜研究所, 天津 300192
  • 收稿日期:2004-03-10 出版日期:2004-12-28
  • 作者简介:王晓云(1975-),女,山东菏泽人,在读硕士,主要从事植物生理研究;杜胜利为通讯作者.
  • 基金资助:
    天津市农业生物技术研究中心科技(攻关)计划项目(003122011)

Activities of Peroxidase and Its Isoenzymes in Early Stage of Cucumber Gynogenesis

WANG Xiao-yun1, DU Sheng-li2, CAO Cui-ling1, WEI Ai-min2, HAN Yi-ke2, ZHANG Gui-hua2   

  1. 1. Northwest Sci-tech University of Agriculture and Forestry, Yangling712100, China;
    2. Tianjin Kernel Cucumber Research Institute, Tianjin300192, China
  • Received:2004-03-10 Published:2004-12-28

摘要: 以离体培养的黄瓜未受精子房为试材,测定了黄瓜离体雌核发育早期过氧化物酶及其同工酶的活性。结果表明,在培养的09d,总的过氧化物酶(POD)、NADH氧化酶(NADHPOD)和生长素氧化酶(IAAO)活性均呈上升趋势;细胞色素氧化酶(COX)、抗坏血酸氧化酶(ASP)活性均呈下降趋势;谷胱甘肽过氧化物酶(GSH Px)则先升后降。雌核发育早期过氧化物酶及其同工酶活性,尤其是NADHPOD和GSH Px活性的急剧升高是雌核发育的重要标志。

关键词: 黄瓜, 雌核发育, 过氧化物酶, 同工酶, 离体培养

Abstract: Peroxidase and its isoenzymes activities were measured in gynogenesis of cucumber ovary cultured for 0-9 days. The results showed that the activities of total peroxidase ( POD), NADH peroxidase ( NADH- POD), indole-3-acetic acid peroxidase ( IAAO) were all increasing markedly. While cytochrome peroxidase ( COX) and ascorbate peroxidase ( ASP) always decreased.And glutathione peroxidase ( GSH-Px) increased first , then decreased. These results indicated that it was signs of cucumber gynogenesis that activities of total perox-i dase and its isoenzymes, especially NADHPOD and GSH-Px increased surprisely.

Key words: Cucumber, Gynogenesis, Peroxidase, Isoenzymes, Isolated culture

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引用本文

王晓云, 杜胜利, 曹翠玲, 魏爱民, 韩毅科, 张桂华. 黄瓜雌核发育早期过氧化物同工酶活性的变化[J]. 华北农学报, 2004, 19(4): 55-58. doi: 10.3321/j.issn:1000-7091.2004.04.015.

WANG Xiao-yun, DU Sheng-li, CAO Cui-ling, WEI Ai-min, HAN Yi-ke, ZHANG Gui-hua. Activities of Peroxidase and Its Isoenzymes in Early Stage of Cucumber Gynogenesis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2004, 19(4): 55-58. doi: 10.3321/j.issn:1000-7091.2004.04.015.

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