抗病毒剂VA诱导烟草产生PR蛋白及对TMV侵染的抗性

doi:10.3321/j.issn:1000-7091.2002.z1.011

摘要/Abstract

摘要： 本试验用天然抗病毒剂VA作诱导因子,抗病品种枯斑三生烟(NicotianatabacumLSamSunNN)和感病品种三生烟(NicotianatabacumLSamSun)经VA诱导处理后(清水作对照)接种TMV。结果表明,VA可局部和系统地诱导烟草叶片对TMV产生抗性,其枯斑抑制率可达40%～60%,枯斑直径比对照减少30%～50%,且在抗病品种SamSunNN中,VA+TMV处理明显增加了PR蛋白的含量,在不接种情况下VA诱导的烟草叶片中PR蛋白表现很弱。在感病品种SamSun烟中,VA不能诱导PR蛋白产生。说明VA诱导PR蛋白含量的增加与VA抑制TMV在烟草体内的扩展有关。

关键词: 烟草, TMV, 系统诱导抗性, 局部诱导抗性, PR蛋白, 抗病毒剂

Abstract: VA,a natured virus inhibitor from several kinds of plants,was used as a elicitor.On the seventh day after treatment with VA,the newly developed leaves of Nicotiana tabacum L.SamSunNN and SamSun were inoculated with TMV.Result showed that localized and systemic resistance to TMV were induced in Nicotiana tabacum L.by VA.Local lesion inhibition rate was 40%-60%.Lical lesion diameter inhibition rate was 30%-50%.From 48 h to 72 h after treated with VA+TMV in SamSun NN tabacco plants,PR proteins were weaker in the TMV noninfected SamSun NN;Moreover,in SamSun tabacco plants,VA could not induce PR proteins with inoculation or noinoculation treatment.It indicated that VA could inhibite infection and replication of TMV.

Key words: Tobacco, TMV Systemic induced resistance Localized induced resistance PR proteins Virus inhibitor

中图分类号:

S435.12

赵春颖, 王凤茹, 李兴红. 抗病毒剂VA诱导烟草产生PR蛋白及对TMV侵染的抗性[J]. 华北农学报, 2002, 17(S1): 50-55. doi: 10.3321/j.issn:1000-7091.2002.z1.011.

ZHAO Chun-ying, WANG Feng-ru, LI Xing-hong. The Virus Inhibitor VA Induces Production of PR Proteins and Resistance to TMV Infection in Tobacco[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(S1): 50-55. doi: 10.3321/j.issn:1000-7091.2002.z1.011.

http://www.hbnxb.net/CN/Y2002/V17/IS1/50

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