摘要： 烟草愈伤组织在再生过程中淀粉粒的数量是继代过程中的8.22倍，淀粉酶活性为4.48倍；胡萝卜中则无显著变化，分别为0.95和1.13倍。再生过程中，烟草愈伤组织中淀粉粒的数量、淀粉酶的活性分别为胡萝卜的6.37和6.14倍；继代过程中二者无显著差异，分别为0.74和1.55倍。烟草细胞中的淀粉粒被I2-KI染成蓝色，于质体中单个存在，在细胞内分布不均匀，常绕细胞核聚集；胡萝卜细胞中的淀粉粒被I2-KI染成紫色或红紫色，2~4个或更多个聚集于质体内，在细胞内基本均匀分布。对淀粉与植物细胞离体再生途径决定的关系进行了讨论。

关键词: 烟草, 胡萝卜, 淀粉代谢, 离体形态建成途径

Abstract: The present paper studied the effect of starch metabolism on the determination of morphogenetic pathway of plant cells in vitro. In the cells of tobacco calli, the average value of starch amount during regenerating was 8.22 times as much as that of subcultures, and the amylase activities during regenerating was 4.48 times higher than that of subcultures. In regenerating calluses of carrot, the starch amount was 0.95 times more and the amylase activities were 1.13 times higher than that of subcultures. During regeneration, the average value of starch amount and amylase activities of tobacco were 6.37 times more and 6.14 times higher than that of carrot, but were 0.74 times and 1.55 times during subculture, respectively. Moreover, starch grains of tobacco cells accumulated in single, stained by I2-KI into blue or dark blue;but in carrot cells, 2-4 or more starch grains aggregated in one plastid, and stained into red to violet. The relationship of morphogenetic pathway determination of plant cells in vitro and the level of starch metabolism, structure features and accumulation pattern of starch grains was discussed.

Key words: Nicotiana tabacum L, Daucus carota L, Starch metabolism, Morphogenetic pathway in vitro