摘要： 应用随机扩增多态性DNA（RAPD）标记鉴定大白菜杂种商品种子的纯度。用50个随机引物检测了2个杂交种北京57号和北京106号及其亲本，引物OPE-20在北京57号杂交种中产生了有别于双亲的特殊标记，引物OPH-06及OPH-07在北京106杂交种中产生了有别于双亲的特殊标记，能清楚地区分杂交种及其双亲，并将这些引物应用在北京57号和北京106号杂交种样品的纯度检测中。这个结果显示了RAPD标记在大白菜杂交种商品种子纯度检测上的实际用途。

关键词: 大白菜, RAPD标记, 杂交种纯度

Abstract: Randomly amplified polymorphic DNA(RAPD)markers were applied to distinguish purity of commercial F1-hybrid seeds of Chinese cabbage. DNA from two F1-hybrid cultivars, Beijing No.57 and Beijing No.106, and their parental lines were screening with 50 primers. Primer OPE-20 generated in F1-hybrid Beijing No.57 specific RAPD markers. Primer OPH-06 and OPH-07 generated in F1-hybrid Beijing No.106 specific RAPD markers. These markers enabled a clear distinction to be made between F1-hybrids and their parental lines. These primers were used in purity control of hybrid seed production of Beijing No.57 and Beijing No.106 . This result indicated the practical usefulness of RAPD markers in commercial hybrid seeds purity tests of Chinese cabbage.

Key words: Chinese cabbage, RAPD marker, Hybrid purity