华北农学报 ›› 1995, Vol. 10 ›› Issue (S1): 33-37. doi: 10.3321/j.issn:1000-7091.1995.z1.007

所属专题: 烟草 黄瓜 生物技术

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抗黄瓜花叶病转基因烟草新品系的对比及示范试验

吴中心1, 沈金昌2, 王振坤2, 杨永斌2, 王廷晓3, 周瑞宽3   

  1. 1. 河南省农业科学院烟草研究所, 许昌, 461100;
    2. 河南省禹州市烟草公司;
    3. 河南省襄城县烟草公司
  • 收稿日期:1994-09-11 出版日期:1995-12-31

Virus Resistance and Cultivar Properties in Transgenic Tobacco Lines under Field Conditions

Wu Zhongxin1, Shen Jinchang2, Wang Tingxiao2, Wang Zhenkun2, Yang Yongbin3, Zhou Ruikuan3   

  1. 1. Institute of Tobacco, Henan Academy of Agricultural Sciences, Xuchang, 461000;
    2. Tobacco Corporation of Yuzhou City, Henan Province;
    3. Tobacco Corporation of Xiangcheng County, Henan Province
  • Received:1994-09-11 Published:1995-12-31

摘要: 对同时表达黄瓜花叶病毒外壳蛋白(CMV CP)和卫星RNA的转基因烟草NC89新品系(NC89-B)及单一表达CMV CP的转基因NC89新品系(NC89-T),在大田生产条件下进行了品比及示范试验。结果表明,在抗病性方面,转基因NC89明显高于未转基因NC89,其中B品系对CMV的相对防效达80%左右,T品系对CMV的相对防效达20%左右;在农艺性状方面,转基因NC89较对照有较显著改善,产值、产量、上等烟比例均有提高,但种性未变;在原烟品质方面,转基因NC89与对照基本一致。

关键词: 烟草, 转基因NC89, 抗病性, 农艺性状

Abstract: The resistance to cucumber mosaic virus and the preservation of cultivar properties in transgenic tobacco cv NC89-B line expressing "CMV CP + Satellite RNA" and NC89-T line possessing CMV CP gene were tested under field conditions.The results indicated that the transgenic lines showed much higher resistance than the non-transformed plants.The disease index of the transgenic line B, the best line, averaged about 7.9 and 35.1 in contrast to about 40.6 and 72.5 of the non-transformed NC89 under natural condition and with inoculation of viruses, respectively.The agronomic properties of the transgenic lines improved but showed no genetic variation compared with the non-transformed plants.

Key words: Tobacco, Transgenic NC89, Resistance, Agronomic characters

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引用本文

吴中心, 沈金昌, 王振坤, 杨永斌, 王廷晓, 周瑞宽. 抗黄瓜花叶病转基因烟草新品系的对比及示范试验[J]. 华北农学报, 1995, 10(S1): 33-37. doi: 10.3321/j.issn:1000-7091.1995.z1.007.

Wu Zhongxin, Shen Jinchang, Wang Tingxiao, Wang Zhenkun, Yang Yongbin, Zhou Ruikuan. Virus Resistance and Cultivar Properties in Transgenic Tobacco Lines under Field Conditions[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1995, 10(S1): 33-37. doi: 10.3321/j.issn:1000-7091.1995.z1.007.

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