华北农学报 ›› 1994, Vol. 9 ›› Issue (S1): 138-140. doi: 10.3321/j.issn:1000-7091.1994.z1.029

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饲料中棕曲霉毒素A的测定方法

吴坤   

  1. 河南农业大学农学系, 郑州450002
  • 收稿日期:1993-08-11 出版日期:1994-12-31
  • 基金资助:
    国家自然科学基金项目

A Method of Detection of Ochratoxin Ain Mixed Feedstuffs

Wu Kun   

  1. Agronomy Department, Henan Agricultural University, Zhengzhou 450002
  • Received:1993-08-11 Published:1994-12-31

摘要: 通过实验建立了一种饲料中棕曲霉毒素A的薄层层析测定方法。该法用氯仿:甲醇(2:1,V/V)提取样品中的毒素,提取液经分液法净化,在硅胶薄层板上用二次展开系统展开分离后,在紫外光灯(254nm和365nm)下检测定量。该法的方法灵敏度为1Oppb,回收率为70%~100%。

关键词: 饲料, 薄层层析, 真菌毒素, 棕曲霉毒素A

Abstract: method is described for detection of ochratoxin A in mixed feedstuffs.Thetoxin is extracted with and purified by iquidliquid partition.Then,after being separated on silica gel thin layer plate by developing successively.

Key words: Feedstuffs, TLC, M ycotoxin, chratoxin A

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引用本文

吴坤. 饲料中棕曲霉毒素A的测定方法[J]. 华北农学报, 1994, 9(S1): 138-140. doi: 10.3321/j.issn:1000-7091.1994.z1.029.

Wu Kun. A Method of Detection of Ochratoxin Ain Mixed Feedstuffs[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1994, 9(S1): 138-140. doi: 10.3321/j.issn:1000-7091.1994.z1.029.