摘要： 红豆草下胚轴切段在含有1mg/1BA, 1mg/1KT的LS培养基上培养, 两周后在切面处产生愈伤组织。选择由一块切段产生的淡黄色愈伤组织, 繁殖并连续选择几代至其外观稳定, 转移到含1mg/1BA的LS培养基上, 15天后即可观察到有大量的球形胚形成, 但只有少数可按正常的胚胎发育途径发育。其存在的另一可能途径是球芽途径, 即球状结构上逐渐分化出根和芽而得到完整植株, 它具有独立的结构, 不同于一般的器官发生途径。

关键词: 红豆草, 组织培养, 体细胞胚, 克隆

Abstract: Hypocotylar explants of Onobrychis viciaefolia Scop were cultured on LS basal medium-supplemented with 1 mg/l BA, 1mg/l KT. Two weeks after of culture calli were initiated on the surface of sections. Light-yellow callus from one explants was selected and proliferated on LS medium. And then it transferrated to somatic embryo induction medium(LS basal medium with lmg/1 BA). Contact embryogenic calli containing a large number of globular embryos were found after 15 days of embryogenic culture, but only a few globular embryos can develop normally. Another new possible pathway for in vitro development is. buds and roots were formed on the surface of globular-like structure and it is different from normal organogenesis because of its individual structure.

Key words: Sainfoin, Tissue culture, Somatic embryo, Cloning