摘要: 1986-1987年将金丝小枣高产质佳4个品系、成年树上的枣头茎段,使用MS基本培养基培养成试管苗。试验结果表明,外植体的生理状态与培养试管苗有直接关系;试管苗经常出现二次枝,它可以生根,但生芽继代繁殖困难;利用果仁接种在适宜培养基上,可以生长健壮植株,下胚轴可由不定芽发育成小植株。
关键词:
金丝小枣,
枣头,
茎段培养
Abstract: From 1986 to 1987,we cultured the stems of adult trees of four strains,which are highly productive and quality,to test-tube plants by modified MS medium.The results showed there were direct relationships between the physiological status of explants and the test-tube plants;the test-tube plants offen developed second branches which could take root,but could'nt sprout;the plantlets were used to be grafted on a proper medium and could grow to robust plants and the advetltilious buds on hypocotyl could develop to young plants.
Key words:
Jinsi Chinese date,
Chinese date head,
Stem culture
刘贵仁, 严仁玲, 王震星, 张磊, 杨恩琴, 桑子诚. 金丝小枣茎段离体培养及胚培养的研究[J]. 华北农学报, 1988, 3(4): 116-119. doi: 10.3321/j.issn:1000-7091.1988.04.022.
Liu Guiren, Yan Renling, Wang Zhenxing, Zhang Lei, Yang Enqin, Sang Zicheng. The Researchmen of Culturing Stem and Embryo of the Jinsi Chinese Date[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1988, 3(4): 116-119. doi: 10.3321/j.issn:1000-7091.1988.04.022.