Cloning and Analysis of RcDof Gene Associated with Castor Dwarfing
SUN Huajun1, LI Guorui1,2,3,4, HUANG Fenglan1,2,3,4, LI Yue1, CONG Anqi1, LI Wei5, QI Meng5, CHEN Yongsheng1,2,3,4
1. College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, China;
2. Inner Mongolia Industrial Engineering Research Center of Universities for Castor, Tongliao 028000, China;
3. Inner Mongolia Key Laboratory of Castor Breeding, Tongliao 028000, China;
4. Inner Mongolia Collaborate Innovation Cultivate Center for Castor, Tongliao 02800, China;
5. College of Agronomy, Inner Mongolia University for Nationalities, Tongliao 028000, China
Abstract：In order to research the features and biological function of RcDof gene in dwarf castor,stem tip genome DNA was extracted in growth climacteric age.Primers were designed according to obtained Zinc finger protein gene fragment.Using RACE technology,the complete full-length open reading frame of 924 bp was cloned,encoding 307 amino acids,belonged to C2C2 zinc finger protein,predicted protein molecular weight was 34.020 9 kDa,isoelectric point was 9.23.The predicted secondary structure α helix accounted for 1.63%,β fold accounted for 1.30%,other non-random coil accounted 97.07%,belonged to outer release proteins.Subcellular localization showed that the RcDof gene located in the nucleus.The bioinformatics analysis of RcDof gene will lay the foundations for the further study on its action mechanism and function characteristics in the castor dwarfing process.
[1] 郑 鹭,祁建民,陈绍军,等.蓖麻遗传育种进展及其在生物能源与医药综合利用潜势[J].中国农学通报,2006,22(9):109-113.
[2] 黄志银,黄爱玲,刘红光,等.优质高产淄蓖系列新品种[J].农业科技通讯,2002(7):41.
[3] Chan A P,Crabtree J,Zhao Q,et al.Draft genome sequence of the oilseed species Ricinus communis Nat Biotech[J].Nature Biotechnology,2010,28(9):951-956.
[4] 蔡晓锋,张余洋,张俊红,等.植物Dof基因家族功能研究进展[J].植物生理学报,2013,49(1):1-12.
[5] Pedone P V,Ghirlando R,Clore G M,et al.The single Cys2-His2 zinc finger domain of the GAGA protein flanked by basic residues is sufficient for high-affinity specific DNA binding[J].Proceedings of the National Academy of Sciences,1996,93(7):2822-2826.
[6] Baglivo I,Palmieri M,Rivellino A,et al.Molecular strategies to replace the structural metal site in the prokaryotic Zinc finger domain[J].Biochimica et Biophysica acta,2014,1844(3):497-504.
[7] Lindorff-Larsen K,Piana S,Dror R O,et al.How Dof fast-folding proteins fold[J].Science,2011,334(6055):517-520.
[8] Santos L A,Souza S R,Fernandes M S.OsDof25 expression alters carbon and nitrogen metabolism in Arabidopsis under high N-supply[J].Plant Biotechnol Rep,2012,6:327-337.
[9] Chen X Y,Wang D X,Liu C,et al.Maize transcription factor Zmdof1 involves in the regulation of Zm401 gene[J].Plant Growth Regulation,2012,66(3):271-284.
[10] Negi J,Moriwaki K,Konishi M,et al.A Dof transcription factor,SCAP1,is essential for the development of functional stomata in Arabidopsis[J].Current Biology,2013,23(6):479-484.
[11] Wei P C,Tan F,Gao X Q,et al.Overexpression of AtDOF4.7,an Arabidopsis DOF family transcription factor,induces floral organ abscission deficiency in Arabidopsis[J].Plant Physiology,2010,153(3):1031-1045.
[12] Gaur V S,Singh U S,Kumar A.Transcriptional profi ling and in silico analysis of Dof transcription factor gene family for understanding their regulation during seed development of rice Oryza sativa L.[J].Mol Biol Rep,2011,38(4):2827-2848.
[13] Kushwaha H,Gupta S,Singh V K,et al.Genome wide identification of Dof transcription factor gene family in sorghum and its comparative phylogenetic analysis with rice and Arabidopsis[J].Mol Biol Rep,2014,16(4):372-384.
[14] Gupta N,Gupta A K,Kumar A.Spatial distribution pattern analysis of Dof1 transcription factor in different tissues of three Eleusine coracanagenotypes differing in their grain colour,yield and photosynthetic efficiency[J].Molecular Biology Reports,2012,39(3):2089-95.
[15] Guo Y,Qiu L J.Genome-wide analysis of the Dof transcription factor gene family reveals soybean-specific duplicable and functional characteristics[J].PLoS One,2013,8(9):e76809.
[16] 连肖华,陈 坚.SUSIRI基因的生物信息学分析及亚细胞定位[J].中国农学通报,2015,31(6):128-135.
[17] Tark D H,Sim P O,Kim J S,et al.The Arabidopsis Ran gene encodes Dof domain transcription factor [J].Plant J,2013,34:161-171.
[18] Rueda-Romero P,Barrero-Sicilia C,Gómez-Cadenas A,et al.Arabidopsis thaliana DOF6 negatively affects germination in non-after-ripened seeds and interacts with TCP14[J].Journal of Experimental Botany,2012,63(5):1937-1949.
[19] Skirycz A,Jozefczuk S,Stobiecki M,et al.Transcription factor AtDOF4;2 affects phenylpropanoid metabolism in Arabidopsis thaliana[J].The New Phytologist,2007,175(3):425-438.
[20] Baumann K,De Paolis A,Costantino P,et al.The DNA binding site of the Dof protein NtBBF1 is essential for tissue-specific and auxin-regulated expression of the rolB oncogene in plants[J].The Plant Cell,1999,11(3):323-334.
[21] Wang H W,Zhang B,Hao Y J,et al.The soybean Dof-type transcription factor genes,GmDof4 and GmDof11,enhance lipid content in the seeds of transgenic Arabidopsis plants[J].The Plant Journal:for Cell and Molecular Biology,2007,52(4):716-729.
2016, Vol. 31 
