蓖麻矮化相关基因<em>RcDof</em>的克隆及分析

doi:10.7668/hbnxb.2016.04.011

华北农学报 ›› 2016, Vol. 31 ›› Issue (4): 63-67. doi: 10.7668/hbnxb.2016.04.011

所属专题： 生物技术；

蓖麻矮化相关基因RcDof的克隆及分析

孙华军¹, 李国瑞^1,2,3,4, 黄凤兰^1,2,3,4, 李跃¹, 丛安琪¹, 李威⁵, 齐蒙⁵, 陈永胜^1,2,3,4

1. 内蒙古民族大学生命科学学院, 内蒙古通辽 028000;
2. 内蒙古自治区高校蓖麻产业工程技术研究中心, 内蒙古通辽 028000;
3. 内蒙古自治区蓖麻育种重点实验室, 内蒙古通辽 028000;
4. 内蒙古自治区蓖麻产业协同创新培育中心, 内蒙古通辽 028000;
5. 内蒙古民族大学农学院, 内蒙古通辽 028000

收稿日期:2016-03-21 出版日期:2016-08-28
通讯作者: 陈永胜(1971-),男,内蒙古通辽人,教授,博士,主要从事蓖麻分子育种研究。
作者简介:孙华军(1992-),女,内蒙古呼伦贝尔人,在读硕士,主要从事蓖麻分子育种研究。
基金资助:
国家自然科学基金项目（31460353）；2015年全区研究生科研创新资助项目（S20151013601）；内蒙古民族大学市校合作项目（SXZD2012006；SXZD2012017）；内蒙古自治区科技创新引导资金项目（KJCX15002）；内蒙古自治区蓖麻产业协同创新培育中心项目；内蒙古自治区蓖麻育种重点实验室开放基金项目（MDK2016030）

Cloning and Analysis of RcDof Gene Associated with Castor Dwarfing

SUN Huajun¹, LI Guorui^1,2,3,4, HUANG Fenglan^1,2,3,4, LI Yue¹, CONG Anqi¹, LI Wei⁵, QI Meng⁵, CHEN Yongsheng^1,2,3,4

1. College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, China;
2. Inner Mongolia Industrial Engineering Research Center of Universities for Castor, Tongliao 028000, China;
3. Inner Mongolia Key Laboratory of Castor Breeding, Tongliao 028000, China;
4. Inner Mongolia Collaborate Innovation Cultivate Center for Castor, Tongliao 02800, China;
5. College of Agronomy, Inner Mongolia University for Nationalities, Tongliao 028000, China

Received:2016-03-21 Published:2016-08-28

摘要/Abstract

摘要： 为研究RcDof基因在蓖麻矮化中的作用及生物学功能，提取蓖麻生长跃变期茎尖中基因组DNA，根据蓖麻中已获得的锌指蛋白基因片段设计引物，采用RACE技术克隆得到该基因，基因完整阅读框全长为924 bp，可编码307个氨基酸，为C2C2型锌指蛋白，预测蛋白质分子量为34.020 9 kDa，等电点为9.23。二级结构预测表明α螺旋占1.63%，β折叠占1.30%，其他无规则卷曲占97.07%，为外释放蛋白。亚细胞定位于细胞核中，对RcDof基因的生物信息学分析为进一步研究其在蓖麻矮化过程中的作用机制和功能特征提供理论依据。

关键词: 蓖麻矮化, 基因克隆, 亚细胞定位, 生物信息学分析

Abstract: In order to research the features and biological function of RcDof gene in dwarf castor,stem tip genome DNA was extracted in growth climacteric age.Primers were designed according to obtained Zinc finger protein gene fragment.Using RACE technology,the complete full-length open reading frame of 924 bp was cloned,encoding 307 amino acids,belonged to C2C2 zinc finger protein,predicted protein molecular weight was 34.020 9 kDa,isoelectric point was 9.23.The predicted secondary structure α helix accounted for 1.63%,β fold accounted for 1.30%,other non-random coil accounted 97.07%,belonged to outer release proteins.Subcellular localization showed that the RcDof gene located in the nucleus.The bioinformatics analysis of RcDof gene will lay the foundations for the further study on its action mechanism and function characteristics in the castor dwarfing process.

Key words: Castor dwarfing, Gene clone, Subcellular localization, Bioinformatics analysis

中图分类号:

孙华军, 李国瑞, 黄凤兰, 李跃, 丛安琪, 李威, 齐蒙, 陈永胜. 蓖麻矮化相关基因RcDof的克隆及分析[J]. 华北农学报, 2016, 31(4): 63-67. doi: 10.7668/hbnxb.2016.04.011.

SUN Huajun, LI Guorui, HUANG Fenglan, LI Yue, CONG Anqi, LI Wei, QI Meng, CHEN Yongsheng. Cloning and Analysis of RcDof Gene Associated with Castor Dwarfing[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(4): 63-67. doi: 10.7668/hbnxb.2016.04.011.

http://www.hbnxb.net/CN/Y2016/V31/I4/63

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