Sequence Variation at BoLA-DRB1* Intron 1-exon 2 in Yak and Cattle
TIAN Zhili1, CHEN Jie1, HU Jiang1, LUO Yuzhu1, LIU Xiu1, LI Shaobin1, GUO Shuzhen2, MU Yongjuan2
1. Faculty of Animal Science and Technology, Gansu Agricultural University, Gansu Key Laboratory of Herbivorous Animal Biotechnology, Lanzhou 730070, China;
2. Institute of Animal Husbandry Science of Gannan Prefecture, Hezuo 747000, China
Abstract:To accumulate more molecular genetics materials for revealing stress resistance and disease resistance breeding by testing the sequence variation at DRB1 gene and analyzing genetic parameters in detecting regionin in yak and cattle.Gannan yak,Qinghai yak,Tianzhu white yak,Datong yak and Cattle were used in this study.The polymorphism of intron 1 and exon 2 in BoLA-DRB1 gene were analyzed using PCR-single-strand conformational polymorphism.The results showed that 4 SNPs and 1 insertion/deletion mutation were detected in intron 1,and 17 SNPs were detected in exon 2,and they were highly polymorphism;Between these two regions,twenty-one haplotypes and the linkage disequilibrium phenomenon were found,and haplptypes A-A1,A-B1,B-A1 and B-B1 were most common in yak and cattle.The cluster analysis of DRB1 gene exon 2 showed that yak and other 6 species,cattle and goat were the highest on homology and the phylogenetic distance consistent with their genetic relationship. DRB1 gene intron 1 and exon 2 have high of sequence polymorphism,it might be used as genetic marker in yak and cattle.
[1] 孙东晓,张 沅.反刍家畜主组织相容性复合体的研究进展[J].中国畜牧杂志,2002,38(5):46-47.
[2] Amorena B,Stone W H.Serologically defined (SD) locus in cattle[J].Science,1978,201(4351):159-160.
[3] Park Y H,Joo Y S,Park J Y,et al.Characterization of lymphocyte subpopulations and major histocompatibility complex haplotypes of mastitis-resistant and susceptible cows[J].Journal of Veterinary Science,2004,5(1):29-39.
[4] Wu X X,Yang Z P,Wang X L,et al.Restriction fragment length polymorphism in the exon 2 of the BoLA-DRB3 gene in Chinese Holstein of the South China[J].Journal of Biomedical Science and Engineering,2010,03(02):221-225.
[5] Aida Y,Takeshima S N,Matsumoto Y,et al.Bovine MHC class II Dr molecule plays a key role in Bovine leukemia virus(BLV)-induced lymphoma[J].Retrovirology,2011,8(1):1-1.
[6] Maillard J C,Berthier D,Chantal I,et al.Selection assisted by a BoLA-DR/DQ haplotype against susceptibility to bovine dermatophilosis[J].Genetics,Selection,Evolution,2003,35(l 1):193-200.
[7] Pandya M,Rasmussen M,Hansen A,et al.A modern approach for epitope prediction:identification of foot-and-mouth disease virus peptides binding bovine leukocyte antigen (BoLA) class I molecules[J].Immunogenetics,2015,67(11/12):691-703.
[8] 赖淑苹,任惠民,胡海涛,等.中国西北地区汉、回、维、藏民族HLA-DRB基因多态性的研究[J].遗传学报,1999,26(5):447-457.
[9] Konnai S,Nagaoka Y,Takeshima S,et al.Sequences and diversity of 17 new Ovar-DRB1 alleles from three breeds of sheep[J].European Journal of Immunogenetics,2003,30(4):275-282.
[10] Gruszczyńska J,Brokowska K,Charon K M,et al.Restriction fragment length polymorphism of exon 2 Ovar-DRB1 gene in Polish heath sheep and Polish lowland sheep[J].Journal of Applied Genetics,2005,46(3):311-314.
[11] 温青娜,贾 斌,申 红,等.哈萨克绵羊MHC-DRB1基因SSCP多态性与细粒棘球蚴病遗传抗性关联分析[J].中国人兽共患病学报,2010,26(9):805-809.
[12] 李 莉,李成涛,柳 燕,等.HLA-DRB1基因分型芯片的法医物证学应用价值研究[J].法医学杂志,2004,20(2):81-84,87.
[13] 亢孝珍,额尔敦木图,姜建强,等.主要组织相容性复合体(MHC)基因研究进展[J].中国畜牧兽医,2014,41(5):28-33.
[14] Zhou H,Hickford J G,Fang Q.A two-step procedure for extracting genomic DNA from dried blood spots on filter paper for polymerase chain reaction amplification[J].Analytical Biochemistry,2006,354(1):159-161.
[15] Byun S O,Fang Q,Zhou H,et al.An effective method for silver-staining DNA in large numbers of polyacrylamide gels[J].Analytical Biochemistry,2009,385(1):174-175.
[16] Hu J,Zhou H T,Smyth A,et al.Polymorphism of the bovine ADRB3 gene[J].Molecular Biology Reports,2010,37(7):3389-3392.
[17] 李少斌.放牧型羔羊肉生产优化杂交组合筛选和藏绵羊DRB1基因第2外显子多态性分析[D].兰州:甘肃农业大学,2010:45-47.
[18] 王佳泰,王继卿,胡 江,等.河西绒山羊GOLA-DRB1基因第2外显子变异特征分析[J].生物技术通报,2011(12):132-138.
[19] 杨清芳,李祥龙,周荣艳,等.不同物种MHC-DRB1基因外显子2生物信息学分析[J].黑龙江畜牧兽医,2010(13):11-13.
[20] 陈月娥,李建华,苟亚峰,等.绵羊和人主要组织相容性复合体DRB1基因外显子2多态性及生物信息学分析[J].中国畜牧兽医,2014,41(4):66-70.
[21] 贾 斌,申 红,余智勇,等.多浪羊和中国美利奴羊MHC-DRB1基因多态性与包虫病的遗传易感性[J].中国人兽共患病学报,2007,23(10):1004-1008,1012.
[22] Harrison P M,Hegyi H,Balasubramanian S,et al.Molecular fossils in the human genome:identification and analysis of the pseudogenes in chromosomes 21 and 22[J].Genome Research,2002,12(2):272-280.
[23] Zhang Z,Harrison P M,Liu Y,et al.Millions of years of evolution preserved:a comprehensive catalog of the processed pseudogenes in the human genome[J].Genome Research,2003,13(12):2541-2558.
[24] Hickford J,Zhou H,Fang Q.Haplotype analysis of the DQA genes in sheep:Evidence supporting recombination between the loci[J].Journal of Animal Science,2007,85(3):577-582.
[25] 成述儒.藏绵羊DQA1、DQA2和DRB3基因多态性与单倍型连锁分析[D].兰州:甘肃农业大学,2010.
[26] 张洪波,赖江华,李生斌.西北地区汉族人群HLA-A、-B、-DRB1基因座单倍型分析[J].中华医学遗传学杂志,2005,22(4):464-466.
[27] 郭松长,刘建全,祁得林,等.牦牛的分类学地位及起源研究:mtDNA D-loop序列的分析[J].兽类学报,2006,26(4):325-330.