EMS诱变亚麻荠获得2S贮藏蛋白缺失突变体

doi:10.7668/hbnxb.2016.03.002

华北农学报 ›› 2016, Vol. 31 ›› Issue (3): 11-17. doi: 10.7668/hbnxb.2016.03.002

EMS诱变亚麻荠获得2S贮藏蛋白缺失突变体

苑丽霞^1,2, 郝敬云², 赵奎², 薛金爱², 李润植²

1. 晋中学院生物科学与技术学院, 山西晋中 030600;
2. 山西农业大学分子农业与生物能源研究所, 山西太谷 030801

收稿日期:2016-03-01 出版日期:2016-06-28
通讯作者: 李润植(1959-),男,山西芮城人,教授,博士,博士生导师,主要从事分子遗传与基因工程研究。
作者简介:苑丽霞(1979-),女,山西武乡人,副教授,在读博士,主要从事分子遗传与基因工程研究。
基金资助:
国家自然科学基金项目(30971806;31201266;31401430;31501323);国家"948"项目(2014-Z39);山西省煤基重点科技攻关项目(FT-2014-01);山西省高校科技创新项目(2016171);山西省高校大学生创新创业项目(2016413)

An EMS-induced Mutant with 2S Protein Deficiency in Seed Storage Proteins of Camelina sativa

YUAN Lixia^1,2, HAO Jingyun², ZHAO Kui², XUE Jinai², LI Runzhi²

1. College of Biological Science and Technology, Jinzhong University, Jinzhong 030600, China;
2. Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu 030801, China

Received:2016-03-01 Published:2016-06-28

摘要/Abstract

摘要： 亚麻荠种子富含大量不饱和脂肪酸和贮藏蛋白。为了获得广泛的育种材料,创建优异的新种质,采用EMS(甲基磺酸乙酯)对亚麻荠种子进行诱变处理。通过EMS不同处理时间(0,8,12,16 h)和不同处理浓度(0,0.2%,0.4%,0.6%,0.8%,1.0%,1.2%)条件下诱变亚麻荠种子,建立了EMS诱变亚麻荠种子的优化体系,以0.8%(V/V)EMS处理成熟种子12h效果最佳,筛选获得一个亚麻荠2SP1突变体。与野生型相比,该突变体种子贮藏蛋白成分中缺失了2S清蛋白,但是亚麻荠种子含油量和蛋白含量、株型和种子萌发等农艺性状未发生显著变化。这种特殊的亚麻荠2SP1突变体可作为研究种子贮藏蛋白合成机理及培育高营养品质亚麻荠新品种的优异种质资源,也可用于进行相关功能基因组学等的研究。

关键词: 亚麻荠, 2S清蛋白, EMS, 突变体(2SP1)

Abstract: Camelina sativa is an important oilseed crop, whose seeds is full of high content of unsaturated fatty acids and contain lots of storage proteins.In order to obtain extensive breeding material and establish superior new germplasm,camelina seeds were treated by EMS (ethyl methane sulphonate).In this study, Camelina sativa seeds were treated for mutation by EMS (ethyl methane sulphonate).The treatments were conducted with different EMS concentrations (0,0.2%,0.4%,0.6%,0.8%,1.0%,1.2%) and various treating times (0,8,12,16 h).An optimizational mutagenesis system by EMS was developed for Camelina sativa seeds.One mutant showing 2S albumin deficiency in seed proteins was obtained,and designated as 2SP1 .Except for this feature,the mutant exhibited no significant difference from the wild-type camelina for other agronomic traits including seed oil and protein contents,plant morphology and seed germination.This special mutant of 2SP1 could be used as a new excellent germplasm for investigating the mechanism of seed storage protein biosynthesis and accumulation,for breeding new Camelina sativa varieties with high nutrition quanlity,and also for further functional genomics research.

Key words: Camelina sativa, 2S albumin/napin, EMS, Mutant (2SP1)

中图分类号:

苑丽霞, 郝敬云, 赵奎, 薛金爱, 李润植. EMS诱变亚麻荠获得2S贮藏蛋白缺失突变体[J]. 华北农学报, 2016, 31(3): 11-17. doi: 10.7668/hbnxb.2016.03.002.

YUAN Lixia, HAO Jingyun, ZHAO Kui, XUE Jinai, LI Runzhi. An EMS-induced Mutant with 2S Protein Deficiency in Seed Storage Proteins of Camelina sativa[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 11-17. doi: 10.7668/hbnxb.2016.03.002.

http://www.hbnxb.net/CN/Y2016/V31/I3/11

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