绣球<i>HmMYB73</i>生物信息学分析及其耐铝功能验证

doi:10.7668/hbnxb.20195913

摘要/Abstract

摘要：

绣球是一种高耐铝且铝积累能力强的观赏植物,花色易受土壤pH值和萼片Al³⁺含量影响,但其耐铝机制尚不十分清楚。MYB转录因子家族在植物逆境响应中具有重要作用,为研究其在绣球耐铝中的功能,以大叶绣球无尽夏为材料,对HmMYB73基因进行克隆、生物信息学、共表达网络、表达模式分析以及酵母功能验证。结果表明,HmMYB73编码266个氨基酸,具有典型的R2R3结构域,属于R2R3-MYB亚家族;与HmMYB73共表达的基因参与催化、转运、应激响应和解毒等生物学功能;铝处理下,HmMYB73在绣球根、叶和萼片中均显著上调表达,其中根部表达量最高,是对照的16倍;过表达HmMYB73可显著增强酵母对铝胁迫的耐受性。综上,HmMYB73可能参与绣球对铝胁迫的生物调节过程。

关键词: 绣球, MYB73, 耐铝性, 共表达网络, 酵母功能验证

Abstract:

Hydrangea macrophylla is an ornamental plant with high aluminum(Al) tolerance and strong Al accumulation capacity.Its flower color is easily influenced by soil pH and the Al³⁺ content in sepals,yet the mechanisms underlying its Al tolerance remain poorly understood.The MYB transcription factor family plays a crucial role in plant stress responses.To investigate the function of MYB transcription factors in Al tolerance in H.macrophylla,this study used H.macrophylla 'Endless Summer' as experimental material.Gene cloning,bioinformatics,co-expression network,expression pattern analysis and yeast functional validation of HmMYB73 were conducted.The results showed that HmMYB73 encoded 266 amino acids,contained a typical R2R3 domain,and belonged to the R2R3-MYB subfamily.Genes co-expressed with HmMYB73 were involved in biological functions such as catalytic activity,transporter activity,response to stimulus and detoxification.Under Al treatment,HmMYB73 was significantly upregulated in the roots,leaves,and sepals of H.macrophylla,with the highest expression level observed in the roots,which was 16 times that of the control.Furthermore,overexpression of HmMYB73 significantly enhanced yeast tolerance to aluminum stress.In summary,HmMYB73 may be involved in the biological regulation during aluminum stress in H.macrophylla.

Key words: Hydrangea macrophylla, MYB73, Aluminum tolerance, Co-expression network, Yeast functional validation

中图分类号:

陈双双, 刘一鸣, 陈文娟, 冯景, 陈慧杰, 刘欣童, 杜晓华, 邓衍明. 绣球HmMYB73生物信息学分析及其耐铝功能验证[J]. 华北农学报, 2025, 40(6): 85-91. doi: 10.7668/hbnxb.20195913.

CHEN Shuangshuang, LIU Yiming, CHEN Wenjuan, FENG Jing, CHEN Huijie, LIU Xintong, DU Xiaohua, DENG Yanming. Bioinformatics Analysis and Functional Validation of Aluminum Tolerance of HmMYB73 in Hydrangea macrophylla[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(6): 85-91. doi: 10.7668/hbnxb.20195913.

http://www.hbnxb.net/CN/Y2025/V40/I6/85

图/表 8

表1 本研究所用引物

Tab.1 The primers used in this study

引物名称 Name	引物序列(5'—3') Sequence (5'—3')
HmMYB73-RTF	ATACAATCAAATCGGCCGCC
HmMYB73-RTR	CCAATGGCTTCACTGTGCAA
HmUPL7-RTF	GCCACGTACTCACACTTGCT
HmUPL7-RTR	TCTGTGCCTGCAATCCCTTT
HmMYB73-F	ATGGTGTTTCCGGCGAGGAT
HmMYB73-R	CTCAATCCTGCTAATCTCAATACGC
HmMYB73-PF	GGGAATATTAAGCTTGGTACCATGGTGTTTCCGGCGAGGAT
HmMYB73-PR	TAGATGCATGCTCGAGCGGCCGCCTCAATCCTGCTAATCTCAATACGC

图1 HmMYB73潜在的磷酸化位点

Fig.1 Potential phosphorylation site of HmMYB73

图2 HmMYB73三级结构

Fig.2 The tertiary structure of HmMYB73

图3 MYB73系统发育树

Fig.3 Phylogenetic tree of MYB73

图4 MYB73氨基酸序列比对

Fig.4 Amino acid sequence comparison of MYB73

图5 绣球HmMYB73共表达网络

Fig.5 Co-expression network of hydrangea HmMYB73

图6 铝胁迫下HmMYB73在绣球中的表达模式不同字母表示不同处理之间差异显著(P<0.05)。

Fig.6 Expression level of HmMYB73 in hydrangea under Al treatment Different letters indicate significant differences between different treatment(P<0.05).

图7 HmMYB73可增强酵母的耐铝性 A.酵母表型分析;B.Al₂(SO₄)₃处理下酵母生长曲线。不同字母表示不同时间不同菌株之间差异显著(P<0.05)。

Fig.7 Enhancement of Al tolerance by HmMYB73 in yeast A.Phenotypic analysis of yeast;B.Determination of growth curve of yeast under Al₂(SO₄)₃ treatment. Different letters indicate significant differences between different strains in different time(P<0.05).

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绣球HmMYB73生物信息学分析及其耐铝功能验证

Bioinformatics Analysis and Functional Validation of Aluminum Tolerance of HmMYB73 in Hydrangea macrophylla

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绣球HmMYB73生物信息学分析及其耐铝功能验证

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