摘要：

为筛选板栗叶片中的功能性物质,利用超高效液相色谱串联质谱(LC-MS)对4个板栗品种的成熟叶片进行了检测,结合ChemicalBook、化源网、相关药典及Web of Science数据库筛选出功能性物质,并对重点关注的18种次生代谢物在不同品种间的含量进行比较分析。结果表明,板栗叶片中共检测到673种次生代谢物,包括黄酮、酚酸、生物碱、鞣质、萜类、木质素和香豆素等。PCA和聚类分析表明,燕山早丰和燕奎品种的次生代谢组学轮廓差异较小;对分类贡献率较大的次生代谢物是根皮素、香蜂草苷、柳匍匐次苷、熊果苷、肉桂酸、没食子儿茶素、原花青素B1等,表明代谢组差异可作为区分不同板栗品种的一种重要依据。筛选出功能性次生代谢物共202种,包括51种酚酸、24种生物碱、83种黄酮类、21种萜类、14种木质素和香豆素以及9种鞣质,其中原儿茶酸、山楂酸、熊果酸、鞣花酸、绿原酸、扁蓄苷、山柰酚-7-O-葡萄糖苷、槲皮素、绣线菊苷、紫云英苷、甜菜红苷、银锻苷、香蜂草苷、白麻苷等活性物质在叶片中含量较高,为板栗叶片的资源化利用提供理论依据。

关键词: 板栗, 叶片, 次生代谢物, 功能性物质

Abstract:

To provide a theoretical basis for the resource utilization of Chinese chestnut,the mature leaves of four chestnut cultivars were tested using ultra high performance liquid chromatography-tandem mass spectrometric,and functional secondary metabolites were screened by consulting relevant pharmacopoeias and searching the Chemical Book,Chemsrc,pharmacopeias and Web of Science database. The contents of 18 secondary metabolites we focused on in different cultivars were compared and analyzed. The study showed that a total of 673 kinds of secondary metabolites were detected in chestnut leaves,including flavonoids,phenolic acids,alkaloids,tannins,terpenoids,lignin and coumarin. Principal component analysis and cluster analysis showed that the difference of secondary metabolomics profile between Yanshanzaofeng and Yankui was little. The major secondary metabolites contributing to the classification were phloretin,didymin,salirepin,arbutin,cinnamic acid,gallocatechin,procyanidin B1 and so on. The differential metabolites could also be used as biomarkers to distinguish different chestnut varieties. A total of 202 functional secondary metabolites were selected,including 51 phenolic acids,24 alkaloids,83 flavonoids,21 terpenoids,14 lignins and coumarins,and 9 tannins. The contents of active substances were high in leaves such as protocatechuic acid,maslinic acid,ursolic acid,ellagic acid,chlorogenic acid,avicularin,kaempferol-7-O-glucoside,quercetin,spiraeoside,astragalin,betanin,tiliroside,ditymin and baimaside,which provided reference for the resource utilization of Chinese chestnut leaves.

Key words: Chinese chestnut, Leaf, Secondary metabolite, Functional substance