大白菜抗干烧心病分子标记的开发与验证

doi:10.7668/hbnxb.20192409

摘要/Abstract

摘要： 为开发青麻叶大白菜中抗干烧心病连锁紧密的分子标记，加速优质抗干烧心病品种的培育。以优良的青麻叶高代自交系材料H227和白麻叶高代自交系材料G83同步构建了200份F₂及100份BC₁F₁群体作为试验材料，试验采用离体叶片扦插法对群体的抗感表型进行多次鉴定并统计分析了青麻叶大白菜中干烧心病的遗传规律，同时结合BSA法利用软件JoinMap 4.0和Mapchart对干烧心抗病基因进行初步定位及标记开发。结果发现，由干烧心病抗感差异显著的亲本所构建的F₂群体和BC₁F₁群体病情分级均呈现明显的单峰分布，接近于正态分布，抗性遗传规律具有数量性状遗传特征。基于大白菜基因组数据库的序列信息和所构建的分子标记连锁图谱，试验材料中的抗病基因被定位于A07染色体上，并设计得到1个与抗干烧心病基因相连锁的分子标记BrIDCRT07。该分子标记介于BrID10343和BrID10349之间，与BrID10343的遗传距离为0.13 cM，与BrID10349的遗传距离为0.78 cM。经验证该标记在F₂群体的吻合率达86.8%，BC₁F₁群体吻合率达94.9%，可作为今后大白菜抗干烧心资源的辅助筛选标记。

关键词: 大白菜, 干烧心病, 分子标记, 集团分离分析法

Abstract: In order to develop closely linked molecular markers for resistance to dry burning heart disease in Qingmaye Chinese cabbage and accelerate the cultivation of high-quality varieties resistant to dry burning heart disease.200 F₂ and 100 BC₁F₁ populations were constructed simultaneously with the excellent Qingmaye high generation inbred line H227 and Baimaye high generation inbred line G83,the resistance and susceptibility phenotypes of the population were identified by in vitro leaf cutting method, and the genetic law of dry burning heart disease in Qingmaye Chinese cabbage was statistically analyzed. At the same time, combined with BSA method, the disease resistance gene of dry burning heart was preliminarily located and labeled by software JoinMap 4.0 and Mapchart.The results showed that the disease grading of F₂ population and BC₁F₁ population constructed by parents with significant difference in resistance and susceptibility to dry burning heart disease showed obvious unimodal distribution, close to normal distribution, and the inheritance law of resistance had the genetic characteristics of quantitative traits.Based on the sequence information of Chinese cabbage genome database and the constructed molecular marker linkage map, the disease resistance gene in the experimental material was located on A07 chromosome, and a molecular marker BrIDCRT07 linked to the dry burning heart disease resistance gene was designed.The genetic distance between the molecular marker and BrID10343 and BrID10349 was 0.13, 0.78 cM respectively.It was verified that the coincidence rate of this marker in F₂ population was 86.8%, and that in BC₁F₁ population was 94.9%. It could be used as an auxiliary screening marker for anti dry burning heart disease resources of Chinese cabbage in the future.

Key words: Chinese cabbage, Dry burning heart disease, Molecular markers, Bulked segregant analysis

中图分类号:

张红, 徐莹莉, 王超楠, 黄志银, 范伟强, 李梅, 张斌. 大白菜抗干烧心病分子标记的开发与验证[J]. 华北农学报, 2021, 36(6): 195-203. doi: 10.7668/hbnxb.20192409.

ZHANG Hong, XU Yingli, WANG Chaonan, HUANG Zhiyin, FAN Weiqiang, LI Mei, ZHANG Bin. Development and Validation of Molecular Markers for Resistance to Dry Burning Heart Disease in Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 195-203. doi: 10.7668/hbnxb.20192409.

http://www.hbnxb.net/CN/Y2021/V36/I6/195

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