黄瓜花芽性别分化的研究进展与展望

doi:10.7668/hbnxb.2015.S1.014

摘要/Abstract

摘要： 黄瓜的遗传背景狭窄而性型丰富,是研究植物性别分化的模式植物.一直以来,从形态学到细胞学,从经典遗传学到分子生物学,研究者们对黄瓜的性别分化及其调控机理做了大量研究,揭示了其遗传基础以及以乙烯代谢途径为主线的分子调控机制,但尚不能完全诠释黄瓜性别分化以及性型多样性和多变性的成因.近年来,基因组学、表观遗传学、miRNA等方面研究不断发展和深入,为黄瓜性型分化研究提供了新的方向和技术,有望通过多个层面和角度的综合研究进一步解释黄瓜性别分化与多样性形成的机制,为调控黄瓜性别分化,实现高产稳产栽培和瓜类作物性型分子育种提供理论依据和技术支持.

关键词: 黄瓜, 性别分化, 遗传基础, 分子调控机制, 表观遗传, miRNA

Abstract: Although the genetic background of cucumber is narrow,its sex types are diverse and abundant.Therefore, cucumber is the model plant for sex differentiation study.From morphology to cytology and from classical genetics to molecular biology,researchers have done a lot of important researches on cucumber sex differentiation.It was revealed that the genetic basis of major sex types and the molecular regulation mechanism of ethylene metabolic pathway as main line.But the mechanism of sex differentiation of cucumber flowers and diversification of the sex types still couldn't be completely elucidated.In recent years,the rapid development of genomics,epigenetics and miRNA provided new perspectives and technologies for deeply clarifying the mechanisms of sex differentiation.The further comprehensive analyses would provide theoretic and technical support for regulating sex differentiation in cucumber cultivation of high and stable yield and the molecular breeding for sex type improvement of cucurbitaceae crops.

Key words: Cucumber, Sex differentiation, Genetic basis, Molecular regulation mechanism, Epigenetics, miRNA

中图分类号:

S642.03

张微, 李锡香. 黄瓜花芽性别分化的研究进展与展望[J]. 华北农学报, 2015, 30(S1): 74-80. doi: 10.7668/hbnxb.2015.S1.014.

ZHANG Wei, LI Xi-xiang. The Research Advances on Sex Differentiation of Cucumber Flower Bud[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 74-80. doi: 10.7668/hbnxb.2015.S1.014.

http://www.hbnxb.net/CN/Y2015/V30/IS1/74

参考文献

[1] 谭其猛.蔬菜育种[M].北京:农业出版社,1980.
[2] 窦欣欣.黄瓜核心种质的性型鉴定及复雌性型的遗传分析[D].北京:中国农业科学院,2010.
[3] Malepszy S,Niemirowicz-Szczytt K.Sex determination in cucumber(Cucumis sativus) as a model system for molecular biology[J].Plant Science,1991,80(1/2):39-47.
[4] 李锡香.黄瓜种质遗传多样性的形态和分子评价及其亲缘关系研究[D].北京:中国农业科学院,2002.
[5] Ping-Chin C,Dayakar R P.Morphology and development of the tassel and ear[Z].1994:37-47.
[6] Delong A,Calderon-Urrea A,Dellaporta S L.Sex determination gene TASSELSEED2 of maize encodes a short-chain alcohol dehydrogenase required for stage-specific floral organ abortion[J].Cell,1993,74(4):757-768.
[7] Bai S L,Peng Y B,Cui J X,et al.Developmental analyses reveal early arrests of the spore-bearing parts of reproductive organs in unisexual flowers of cucumber(Cucumis sativus L.)[J].Planta,2004,220(2):230-240.
[8] 白书农,许智宏.从乙烯促雌到乙烯抑雄:黄瓜单性花非正常器官发育命运研究的回顾[J].中国科学:生命科学,2010,40(6):469-475.
[9] 李兴国,李全梓,张宪省.黄瓜性别决定的细胞学研究[J].山东农业大学学报:自然科学版,2001,32(4):411-417.
[10] 袁高峰,赵普庆,孙海燕,等.黄瓜性器官发育过程中显微形态研究及雄花发育晚期SDS-PAGE分析[J].浙江大学学报:农业与生命科学版,2005,31(2):30-35.
[11] 杨玲玲,陈敏,刘复权,等.黄瓜雄花发育过程中心皮原基形态、代谢及基因表达特征的研究[J].科学通报,1999,44(23):2509-2513.
[12] 陈学好.黄瓜花性别分化的生理学研究[D].杭州:浙江大学,2001.
[13] 辛海滨.糖和ABA信号参与低夜温诱导黄瓜性别表达[D].扬州:扬州大学,2013.
[14] 应振土,李曙轩.瓠瓜与黄瓜的性别表现和内源乙烯与氧化酶活性的关系[J].园艺学报,1990,1(1):51-58.
[15] Peng Y B,Zou C,Wang D H,et al.Preferential localization of abscisic acid in primordial and nursing cells of reproductive organs of Arabidopsis and cucumber[J].The New Phytologist,2006,170(3):459-466.
[16] Jiahua Xie,Todd C.Wehner.Gene list 2001 for cucumber[J].Cucurbit Genetics Cooperative Report,2001,24:110-136.
[17] Mibus H,Tatlioglu T.Molecular characterization and isolation of the F/f gene for femaleness in cucumber(Cucumis sativus L.)[J].Theoretical and Applied Genetics,2004,109(8):1669-1676.
[18] 娄群峰.黄瓜全雌性基因分子标记及ACC合酶基因克隆与表达研究[D].南京:南京农业大学,2004.
[19] 陶倩怡,李征,何欢乐,等.黄瓜单性花决定基因M的表达分析[J].遗传,2010(6):632-638.
[20] Yamasaki S,Fujii N,Matsuura S,et al.The M locus and ethylene-controlled sex determination in andromonoecious cucumber plants[J].Plant and Cell Physiology,2001,42(6):608-619.
[21] 刘世强.黄瓜性别决定基因M的精细定位及转录表达谱分析[D].南京:南京农业大学,2008.
[22] Wall J R.Correlated inheritance of sex expression and fruit shape in Cucumis[J].Euphytica,1967,16(2):199-208.
[23] Youngner V B.A study of the inheritance of several characters in the cucumber[D].Saint Paul Minnesota:University of Minnesota,1952.
[24] 陈惠明,刘晓虹.黄瓜性型遗传规律的研究[J].湖南农业大学学报,1999,25(1):42-45.
[25] 时秋香.黄瓜性别决定基因 M 与强雌性基因QTL定位[D].泰安:山东农业大学,2009.
[26] 厉建梅,秦智伟,周秀艳,等.黄瓜雌性性状的遗传分析[J].中国农业科学,2011,44(15):3169-3176.
[27] Kubicki B.Investigation of sex determination in cucumber(Cucumis sativus L.)[J].Trimonoecism.Genet Pol,1969,10:123-143.
[28] Kubicki B.Investigations of sex determination in cucumber(Cucumis sativus L.).V.Genes controlling intensity of femaleness[J].Genetica Polonica,1969,10:69-86.
[29] Kubicki B.Investigation of sex determination in cucumber(Cucumis sativus L.).IX.Induced mutant with the recessive character of gynoecism[J].Genet Pol,1980,21(4):409-424.
[30] Kubicki,B.New sex types in cucumber and their uses in breeding work[C].Proceedings of the XIX_th International Horticultural Congress,1974,3:475-485.
[31] Coen E S,Meyerowitz E M.The war of the whorls:genetic interactions controlling flower development[J].Nature,1991,353(6339):31-37.
[32] Liu Z,Mara C.Regulatory mechanisms for floral homeotic gene expression[J].Seminars in Cell & Developmental Biology,2010,21(1):80-86.
[33] Honma T,Goto K.Complexes of MADS-box proteins are sufficient to convert leaves into floral organs[J].Nature,2001,409(6819):525-529.
[34] Pelaz S,Tapia-López R,Alvarez-Buylla E R,et al.Conversion of leaves into petals in Arabidopsis[J].Current Biology,2001,11(3):182-184.
[35] Theissen G.Development of floral organ identity:stories from the MADS house[J].Current Opinion in Plant Biology,2001,4(1):75-85.
[36] Colombo L,Franken J,Koetje E,et al.The petunia MADS box gene FBP11 determines ovule identity[J].The Plant Cell,1995,7(11):1859-1868.
[37] Kim S,Koh J,Ma H,et al.Sequence and expression studies of A-,B-,and E-class MADS-box homologues in Eupomatia(Eupomatiaceae):Support for the bracteate origin of the calyptra[J].International Journal of Plant Sciences,2005,166(2):185-198.
[38] Becker A,Theissen G.The major clades of MADS-box genes and their role in the development and evolution of flowering plants[J].Molecular Phylogenetics and Evolution,2003,29(3):464-489.
[39] Ando S,Sato Y,Kamachi S,et al.Isolation of a MADS-box gene(ERAF17) and correlation of its expression with the induction of formation of female flowers by ethylene in cucumber plants(Cucumis sativus L.)[J].Planta,2001,213(6):943-952.
[40] 范亚军,李伟.黄瓜 ERAF17 基因的克隆及相关功能载体构建[J].生物技术,2012,22(4):15-18.
[41] Filipecki M K,Sommer H,Malepszy S.The MADS-box gene CUS1 is expressed during cucumber somatic embryogenesis[J].Plant Sci,1997,125:63-74.
[42] Hu L,Liu S.Genome-wide analysis of the MADS-box gene family in cucumber[J].Genome,2012,55(3):245-256.
[43] 李伟. ERAF17 基因在黄瓜雌花分化中的作用[D].长春:东北师范大学,2008.
[44] Yin T J,Quinn J A.Tests of a mechanistic model of one hormone regulating both sexes in Cucumis sativus (Cucurbitaceae)[J].American Journal of Botany,1995,82(12):1537-1546.
[45] Kamachi S,Sekimoto H,Kondo N,et al.Cloning of a cDNA for a 1-aminocyclopropane-1-carboxylate synthase that is expressed during development of female flowers at the apices of Cucumis sativus L[J].Plant and Cell Physiology,1997,38(11):1197-1206.
[46] Trebitsh T,Staub J E,O'neill S D.Identification of a 1-aminocyclopropane-1-carboxylic acid synthase gene linked to the female(F) locus that enhances female sex expression in cucumber[J].Plant Physiology,1997,113(3):987-995.
[47] Shiomi S,Yamamoto M.cDNA cloning of ACC synthase and ACC oxidase genes in cucumber fruit and their differential expression by wounding and auxin[J].Journal of the Japanese Society for Horticultural Science,1998,67(5):685-692.
[48] Kahana A,Silberstein L,Kessler N,et al.Expression of ACC oxidase genes differs among sex genotypes and sex phases in cucumber[J].Plant Molecular Biology,1999,41(4):517-528.
[49] Shiber A,Gaur R K,et al.The origin and mode of function of the female locus in cucumber,cucurbitaceae[J].Proceedings of the IX EUCARPIA meeting on genetics and breeding of Cucurbitaceae,2008,263-270.
[50] 程立宝,秦智伟,李淑艳,等.与黄瓜雌性性状连锁的 cs-acs1g 基因特异片段克隆与鉴定[J].中国农业科学,2006,39(12):2545-2550.
[51] 曲淑娟. Cs-ACS1 转基因对黄瓜性型分化的影响[D].重庆:西南大学,2010.
[52] Li Z,Huang S,Liu S,et al.Molecular isolation of the M gene suggests that a conserved-residue conversion induces the formation of bisexual flowers in cucumber plants[J].Genetics,2009,182(4):1381-1385.
[53] Boualem A,Troadec C,Kovalski I,et al.A conserved ethylene biosynthesis enzyme leads to andromonoecy in two cucumis species[J].PLoS One,2009,4(7):e6144.
[54] Li Z,Wang S,Tao Q,et al.A putative positive feedback regulation mechanism in CsACS2 expression suggests a modified model for sex determination in cucumber(Cucumis sativus L.)[J].Journal of Experimental Botany,2012,63(12):4475-4484.
[55] Yamasaki S,Fujii N,Takahashi H.The ethylene-regulated expression of CS-ETR2 and CS-ERS genes in cucumber plants and their possible involvement with sex expression in flowers[J].Plant & Cell Physiology,2000,41(5):608-616.
[56] Wang D H,Li F,Duan Q H,et al.Ethylene perception is involved in female cucumber flower development[J].The Plant Journal:for Cell and Molecular Biology,2010,61(5):862-872.
[57] 程国辉.光温反应对黄瓜性别分化的影响[D].哈尔滨:东北农业大学,2012.
[58] 马刘峰,易海艳,易霞,等.不同光周期和温度处理对黄瓜花芽分化的影响[J].北方园艺,2012(21):25-26.
[59] 文明玲.光周期和两种诱雄剂处理对黄瓜花性分化及其氧化酶类活性的影响[D].雅安:四川农业大学,2007.
[60] 曹宗巽,李佳格,金以丰,等.在环境因子影响下黄瓜雌雄花比例之改变[J].北京大学学报:自然科学,1957(2):233-246.
[61] Rudich J,Halevy A H,Kedar N.Ethylene evolution from cucumber plants as related to sex expression[J].Plant Physiology,1972,49(6):998-999.
[62] Trebitsh T,Rudich J,Riov J.Auxin,biosynthesis of ethylene and sex expression in cucumber(Cucumis sativus)[J].Plant Growth Regulation,1987,5(2):105-113.
[63] Fujita Y,Fujieda K.Relation between sex expression types and cotyledon etiolation of cucumber in vitro on the role of ethylene evolved from seedlings[J].Plant and Cell Physiology,1981,22:667-674.
[64] 张春平,何平,曲志才,等.硝酸银对黄瓜雌性系的诱雄效应[J].西南大学学报:自然科学版,2007,29(2):49-52.
[65] 曹毅,任吉君,李春梅,等.乙烯利和赤霉素对黄瓜性别表现的影响[J].西南农业大学学报,2002,24(1):42-44.
[66] Peterson C E,Anhder L D.Induction of staminate flowers on gynoecious cucumbers with gibberellin a3[J].Science,1960,131(3414):1673-1674.
[67] Choi Y,Gehring M,Johnson L,et al.DEMETER,a DNA glycosylase domain protein,is required for endosperm gene imprinting and seed viability in Arabidopsis[J].Cell,2002,110(1):33-42.
[68] 袁金玲,郭广平,岳晋军,等.孝顺竹开花过程中DNA甲基化水平动态研究[J].西北植物学报,2012,32(1):60-66.
[69] 孙慧敏.竹类植物衰老与复壮过程中的DNA甲基化研究[D].北京:中国林业科学研究院,2013.
[70] Burn J E,Bagnall D J,Metzger J D,et al.DNA methylation,vernalization,and the initiation of flowering[J].Proceedings of the National Academy of Sciences of the United States of America,1993,90(1):287-291.
[71] 盖树鹏,张风,张玉喜,等.低温解除牡丹休眠进程中基因组DNA甲基化敏感扩增多态性(MSAP)分析[J].农业生物技术学报,2012,20(3):261-267.
[72] 张旭.春化过程对甘蓝型油菜甲基化水平及蛋白质影响的分析[D].郑州:郑州大学,2013.
[73] Aukerman M J,Sakai H.Regulation of flowering time and floral organ identity by a MicroRNA and its APETALA2-like target genes[J].The Plant Cell,2003,15(11):2730-2741.
[74] Chuck G,Meeley R,Irish E,et al.The maize tasselseed4 microRNA controls sex determination and meristem cell fate by targeting Tasselseed6/indeterminate spikelet1[J].Nature Genetics,2007,39(12):1517-1521.
[75] Wu G,Park M Y,Conway S R,et al.The sequential action of miR156 and miR172 regulates developmental timing in Arabidopsis[J].Cell,2009,138(4):750-759.
[76] Baker C C,Sieber P,Wellmer F,et al.The early extra petals1 mutant uncovers a role for microRNA miR164c in regulating petal number in Arabidopsis[J].Current Biology,2005,15(4):303-315.
[77] Cartolano M,Castillo R,Efremova N,et al.A conserved microRNA module exerts homeotic control over Petunia hybrida and Antirrhinum majus floral organ identity[J].Nature Genetics,2007,39(7):901-905.
[78] Nag A,King S,Jack T.miR319a targeting of TCP4 is critical for petal growth and development in Arabidopsis[J].Proceedings of the National Academy of Sciences of the United States of America,2009,106(52):22534-22539.
[79] Allen R S,Li J,Stahle M I,et al.Genetic analysis reveals functional redundancy and the major target genes of the Arabidopsis miR159 family[J].Proceedings of the National Academy of Sciences of the United States of America,2007,104(41):16371-16376.
[80] Nagpal P,Ellis C M,Weber H,et al.Auxin response factors ARF6 and ARF8 promote jasmonic acid production and flower maturation[J].Development,2005,132(18):4107-4118.
[81] Kim J,Jung J H,Reyes J L,et al.microRNA-directed cleavage of ATHB15 mRNA regulates vascular development in Arabidopsis inflorescence stems[J].The Plant Journal,2005,42(1):84-94.

选择文件类型/文献管理软件名称

选择包含的内容