加工番茄早疫病病原菌鉴定

doi:10.7668/hbnxb.2015.S1.057

华北农学报 ›› 2015, Vol. 30 ›› Issue (S1): 316-320. doi: 10.7668/hbnxb.2015.S1.057

所属专题： 番茄；

加工番茄早疫病病原菌鉴定

柴阿丽¹, 石延霞¹, 谢学文¹, 帕提古丽², 李宝聚¹

1. 中国农业科学院蔬菜花卉研究所, 北京 100081;
2. 新疆农业科学院园艺作物研究所, 新疆乌鲁木齐 830091

收稿日期:2015-10-13 出版日期:2015-12-28
通讯作者: 李宝聚(1967-),男,黑龙江饶河人,研究员,博士,主要从事蔬菜病害综合防治研究.
作者简介:柴阿丽(1983-),女,山西运城人,助理研究员,博士,主要从事蔬菜病害诊断研究.
基金资助:
公益性行业(农业)科研专项(201303115);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS);农业部园艺作物遗传改良重点开放实验室项目

Identification of the Pathogens of Processing Tomato Early Blight

CHAI A-li¹, SHI Yan-xia¹, XIE Xue-wen¹, Patiguli², LI Bao-ju¹

1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. Institute of Horticulture and Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2015-10-13 Published:2015-12-28

摘要/Abstract

摘要： 为明确引起加工番茄早疫病的病原菌种类.2013年6-9月,从内蒙古、宁夏和新疆等加工番茄主产区采集了加工番茄早疫病样本46份.采用组织分离法,获得2种真菌分离物,通过形态特征观察、致病性测定、rDNA-ITS序列测定及分析,确定2种真菌分离物分别为茄链格孢和链格孢,分离比例约为2:3.2种病原菌与GenBank 中已有的茄链格孢和链格孢序列相似性均达到100%,序列已在 GenBank上登录,登录号分别为KJ696551、KJ696552和KJ018786、KJ018787.因此,将内蒙古、宁夏和新疆等地的加工番茄早疫病菌鉴定为茄链格孢和链格孢,这是我国首次报道由2种链格孢菌复合侵染引起加工番茄早疫病.

关键词: 加工番茄早疫病, 病原菌鉴定, 茄链格孢, 链格孢, 复合侵染

Abstract: To clarify the pathogens of processing tomato early blight,46 samples of processing tomato early blight were collected from Inner mongolia,Ningxia and Xinjiang autonomous region during Jun.-Sep.2013.The pathogens were isolated from infected tissues,and their pathogenicities were confirmed.They were identified as Alternaria solani (Ell & Mart.) Sorauer and Alternaria alternate (Fr.) Keissler,with an isolated ratio of 2 : 3,based on morphological characters and molecular methods.The rDNA-ITS sequence data of the pathogenic fungi had been submitted to GenBank (GenBank accession number:KJ696551,KJ696552 and KJ018786,KJ018787).Therefore,it confirmed that the pathogens of processing tomato early blight from Inner mongolia,Ningxia and Xinjiang autonomous regions were A.solani and A.alternate,and this was the first report of early blight mixed infected by A.solani and A.alternate in China.

Key words: Processing tomato, Early blight, Alternaria solani, A. alternate, Mixed infection

中图分类号:

S636.3

柴阿丽, 石延霞, 谢学文, 帕提古丽, 李宝聚. 加工番茄早疫病病原菌鉴定[J]. 华北农学报, 2015, 30(S1): 316-320. doi: 10.7668/hbnxb.2015.S1.057.

CHAI A-li, SHI Yan-xia, XIE Xue-wen, Patiguli, LI Bao-ju. Identification of the Pathogens of Processing Tomato Early Blight[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 316-320. doi: 10.7668/hbnxb.2015.S1.057.

http://www.hbnxb.net/CN/Y2015/V30/IS1/316

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