板栗及其近缘种叶绿体SSR遗传多样性分析

doi:10.7668/hbnxb.2015.02.026

华北农学报 ›› 2015, Vol. 30 ›› Issue (2): 145-149. doi: 10.7668/hbnxb.2015.02.026

所属专题： 板栗；

板栗及其近缘种叶绿体SSR遗传多样性分析

程丽莉^1,2, 胡广隆¹, 苏淑钗², 黄武刚¹

1. 北京市农林科学院林业果树研究所, 北京市落叶果树工程技术研究中心, 农业部华北地区园艺作物生物学与种质创制重点实验室, 北京 100093;
2. 北京林业大学森林培育与保护省部共建教育部重点实验室, 北京 100083

收稿日期:2014-12-26 出版日期:2015-04-28
通讯作者: 黄武刚(1958-),男,北京人,研究员,博士,主要从事果树育种研究。
作者简介:程丽莉(1978-),女,河北唐山人,副研究员,博士,主要从事果树资源育种研究。
基金资助:
"十二五"农村领域国家科技计划专题(2013BAD14B0402-1);林业公益行业科研专项(201104025);北京市科委项目(D131100000113001)

Diversity of Cultivated Chestnuts and It's Close Relative Species Using Chloroplast SSRs

CHENG Li-li^1,2, HU Guang-long¹, SU Shu-chai², HUANG Wu-gang¹

1. Institute of Forestry Pomology Academy of Agricultural and Forestry Sciences, Beijing Engineering Research Center for Deciduous Fruit Trees, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China), Ministry of Agriculture, Beijing 100093, China;
2. Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing 100083, China

Received:2014-12-26 Published:2015-04-28

摘要/Abstract

摘要： 为了探明板栗及其近缘种的亲缘关系并分析栗属的遗传多样性,基于叶绿体微卫星标记技术,选用4对呈现多态性的cpSSR引物,对板栗及其近缘种、野生种共6个种,56份材料进行遗传结构、遗传关系等分析。4个位点扩增等位基因数(Na)平均为3.25,有效等位基因数(Ne)平均为2.554,期望杂合度(He)平均为0.606,群体Nei's遗传多样性(Hs)为0.320,各遗传参数值均低于核基因组对群体研究的相应值。另外,各种之间有丰富的cpSSR多样性,尤以野生板栗多样性指数最高。结果表明,我国天然野生板栗群体内蕴含更丰富的遗传变异,为我国板栗野生种质保育及可持续开发利用提供了基础数据和科学依据。

关键词: 板栗, 叶绿体微卫星DNA, 遗传多样性, 序列

Abstract: The objective of the study is to elucidate the genetic relationship and genetic diversity of Castanea species.Based on the technology of chloroplast microsatellite markers, of total 24 cpSSR primer pairs screened, only four presented length polymorphism in the Castanea mollissima tested.We surveyed 6 species, altogether 56 materials with cpSSR markers in order to estimate genetic variation and genetic structure.In the castanea plants, average number of alleles (Na), effective number of alleles (Ne), expected heterozygosity (He) and Nei's were 3.25, 2.554, 0.606 and 0.320, respectively.Apparently, the parameters of cpSSR loci were much lower than those revealed by nuclear SSRs.Furthermore, the intra-specific genetic diversity was high but varied greatly among different species, the wild chestnut in China showed the highest genetic diversity, which one was rich in heredity and variation.The results have important implications of understanding the population genetics and evolutionary patterns in the genus Castanea and provide baseline data for formulating the conservation and management strategies.

Key words: Castanea mollissima BL., cpSSR DNA, Genetic diversity, Sequence

中图分类号:

Q781
P735

程丽莉, 胡广隆, 苏淑钗, 黄武刚. 板栗及其近缘种叶绿体SSR遗传多样性分析[J]. 华北农学报, 2015, 30(2): 145-149. doi: 10.7668/hbnxb.2015.02.026.

CHENG Li-li, HU Guang-long, SU Shu-chai, HUANG Wu-gang. Diversity of Cultivated Chestnuts and It's Close Relative Species Using Chloroplast SSRs[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 145-149. doi: 10.7668/hbnxb.2015.02.026.

http://www.hbnxb.net/CN/Y2015/V30/I2/145

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