一个大豆质子转运体GmMRP5的克隆与表达分析

doi:10.7668/hbnxb.2013.06.006

华北农学报 ›› 2013, Vol. 28 ›› Issue (6): 30-35. doi: 10.7668/hbnxb.2013.06.006

所属专题： 油料作物；

一个大豆质子转运体GmMRP5的克隆与表达分析

许玲, 张大勇, 何晓兰, 徐照龙, 刘晓庆, 黄益洪, 马鸿翔, 易金鑫

江苏省农业生物学重点实验室,江苏省农业科学院农业生物技术研究所, 江苏南京 210014

收稿日期:2013-07-12 出版日期:2013-12-28
通讯作者: 易金鑫(1969－),男,安徽滁州人,研究员,博士,主要从事植物耐盐研究。
作者简介:许玲(1983－),女,安微安庆人,硕士,主要从事大豆耐盐分子机理研究。
基金资助:
国家自然科学基金项目(31101166);江苏省自然科学基金项目(BK2010474);江苏省农业科技自主创新资金项目(cx(11)2052)

Cloning and ExpRession PatteRn Analysis of a PRoton TRanspoRteR GmMRP5 in Soybean

XU Ling, ZHANG Da-yong, HE Xiao-lan, XU Zhao-long, LIU Xiao-qing, HUANG Yi-hong, MA Hong-xiang, YI Jin-xin

Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2013-07-12 Published:2013-12-28

摘要/Abstract

摘要： 为探明大豆中MRP蛋白基因的作用机理,从大豆材料中克隆到GmMRP5基因完整的编码序列,GmMRP5基因的开放阅读框(ORF)全长4 479 bp,编码1 492个氨基酸。序列比对与进化树分析表明:GmMRP5拥有13个推断的跨膜结构域(TMD)和典型的ABC转运蛋白保守结构域,系统进化树分析表明,GmMRP5与AtMRP5同缘关系最近,同属于ABC转运蛋白家族C亚家族;该基因是组成型表达基因,在大豆的根、茎、叶及荚中均有表达;经不同浓度脯氨酸和柠檬酸处理后,该基因在大豆根中的表达被强烈诱导并高效表达。Real-Time PCR结果表明,该基因在酸性缓冲液处理后表达量上调,推测其作为质子转运体参与大豆阴阳离子交换反应。

关键词: 大豆, GmMRP5, 基因克隆, 表达分析

Abstract: In order to investigate the mechanism of the MRP protein genes in soybean, a novel soybean gene GmMRP5 was identified in soybean cultivar.GmMRP5 containing an open reading frame ( ORF) of 4 479 bp nucleo-tides.The ORF encoded a protein of 1 492 amino acids.Sequence alignment and evolutionary tree clustering analy-sis showed that GmMRP5 contains 13 putative transmembrane-spanning domains(TMDs)and ABC transport protein conserved domain.Phylogenetic tree analysis showed that Gm MRP5 was closely related to AtMRP5 and both be-longed to ABCC subfamily.GmMRP5 was a constitutive expression gene and expressed in root, stem and leaves by using Real-Time PCR analysis in soybean.GmMRP5 was strongly induced and highly expressed in root after treat-ment by proline and citrate solution.Real-Time PCR showed the expression level of GmMRP5 was increaced by dif-ferent pH buffer solution treatment, suggesting that GmMRP5 gene may be as a proton transporter to participate in the stress response in soybean.

Key words: Soybean, PRoton tRanspoRteR, Gene cloning, ExpRession analysis

中图分类号:

S565.1

许玲, 张大勇, 何晓兰, 徐照龙, 刘晓庆, 黄益洪, 马鸿翔, 易金鑫. 一个大豆质子转运体GmMRP5的克隆与表达分析[J]. 华北农学报, 2013, 28(6): 30-35. doi: 10.7668/hbnxb.2013.06.006.

XU Ling, ZHANG Da-yong, HE Xiao-lan, XU Zhao-long, LIU Xiao-qing, HUANG Yi-hong, MA Hong-xiang, YI Jin-xin. Cloning and ExpRession PatteRn Analysis of a PRoton TRanspoRteR GmMRP5 in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 30-35. doi: 10.7668/hbnxb.2013.06.006.

http://www.hbnxb.net/CN/Y2013/V28/I6/30

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一个大豆质子转运体GmMRP5的克隆与表达分析

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