摘要： 探讨有机磷杀虫剂对枸杞蚜虫的作用机制，对枸杞蚜虫乙酰胆碱酯酶的底物专一性以及3种有机磷杀虫剂对其的抑制作用进行了研究。结果表明，枸杞蚜虫AChE对乙酰硫代胆碱（ATCh）的水解活性最高，对丁酰硫代胆碱（BuTCh）的亲和力最强，对4种底物的水解趋势相同；3种有机磷杀虫剂对枸杞蚜虫AChE都有抑制作用，其中，氧乐果对枸杞蚜虫AChE体外抑制最强，I50为6．9×10^-6mol／L，辛硫磷和三唑磷分别为9．8×10^-6和11．8×10^-6mol／L；3种有机磷杀虫剂对枸杞蚜虫AChE体外抑制均随着抑制时间的延长，抑制率不断增加。

关键词: 枸杞蚜虫, 乙酰胆碱酯酶, 底物专一性, 有机磷杀虫剂, 抑制

Abstract: The toxicities of organophosphate（OP） insecticides to Aphis sp. were researched in this paper. It was studied that substrate specificity of acetylcholinesterase and the inhibition of organophosphate insecticides to Aphis sp.. The results showed that the activity of AChE from Aphis sp. hydrolyzing ATCh was the highest, while the affinity of AChE to BuTCh was the closest among the four substrates. The hydrolyzing tendencies of AChE from Aphis sp. hy- drolyzing four substrates were similar. It was indicated that 3 OP insecticides tested could inhibitor the activity of AChE in Aphis sp.,and the sensitivity of AChE to omethoate was the highest among the three tested insecticides, and its I50 value was 6.9 × 10^-6mol/L. The values of I50 of phoxin and triazophos to AChE were 9.8 × 10^-6 and 11. 8 × 10^-6mol/L. The inhibitory curses of three insecticides to AChE were same,and their inhibition abilities in- creased with time.

Key words: Aphis sp., Acetylcholinesterase, Substrate specificity, Organophosphate insecticides, Inhibition

中图分类号: 

• S435.622.1