里岔黑猪<em>GDF11</em>基因cDNA的克隆及组织表达谱分析

doi:10.3969/j.issn.1000-7091.2012.01.005

华北农学报 ›› 2012, Vol. 27 ›› Issue (1): 24-29. doi: 10.3969/j.issn.1000-7091.2012.01.005

所属专题： 畜牧；生物技术；

里岔黑猪GDF11基因cDNA的克隆及组织表达谱分析

邢晋祎¹, 戈新², 贾坤航¹, 王建华², 刘忠琛³, 李培培², 张宝珣²

1. 临沂大学生命科学学院, 山东临沂 276005;
2. 青岛市畜牧兽医研究所, 山东青岛 266100;
3. 里岔黑猪原种场, 山东胶州 266300

收稿日期:2011-11-01 出版日期:2012-02-28
通讯作者: 戈新(1957- ), 男, 河北东光人, 研究员, 主要从事畜禽育种与技术推广研究。
作者简介:邢晋祎(1968- ), 男, 山东曹县人, 副教授, 博士, 主要从事动物分子遗传研究。
基金资助:
青岛市基础研究计划项目(09-1-3-64-jch);临沂大学博士启动基金项目(BS08019)

Molecular Cloning and Expression Patterns of GDF11 Gene in Licha Black Pig

XING Jin-yi¹, GE Xin², JIA Kun-hang¹, WANG Jian-hua², LIU Zhong-chen³, LI Pei-pei², ZHANG Bao-xun²

1. College of Life Science, Linyi University, Linyi 276005, China;
2. Institute of Animal Science, Qingdao 266100, China;
3. Seed Stock Station of Licha Black Pig, Jiaozhou 266300, China

Received:2011-11-01 Published:2012-02-28

摘要/Abstract

摘要： 生长分化因子11(Growth and differentiation factor-11,简称GDF11) 是BMP/TGF-β超家族一员,编码一种在早期胚胎发育过程中起重要作用的骨形态发生蛋白,在确立骨骼模式中起重要作用。本研究旨在克隆里岔黑猪GDF11基因部分cDNA,并检测其在不同组织中的表达差异。结果表明,所克隆的猪部分GDF11基因序列为1 161 bp(GenBank:HQ657211),编码332个氨基酸,与人、小鼠、大鼠、牛、马、兔、斑马鱼GDF11氨基酸序列同源性分别为99.10%,99.10%,99.10%,99.40%,99.10%,98.80%,78.82%。荧光定量PCR分析结果显示,GDF11基因mRNA在14种组织中均有表达,总体趋势为:脊椎>背膘>膀胱>肺>脾>胆囊>肾>大肠>心>骨骼肌>肝>小肠>眼肌>胃。这为进一步研究里岔黑猪GDF11基因的结构和功能及其脊椎数发生的机理奠定了基础。

关键词: 里岔黑猪, GDF11基因, 基因克隆, 荧光定量RT-PCR, 组织表达谱

Abstract: GDF11, also known as bone morphogenetic protein 11(BMP11), is a member of the transforming growth factor-β superfamily and BMPs subfamily. It is a secreted protein which is considered to be a key regulator of axial skeleton patterning during early embryogenesis. In this study, the partial cDNA of porcine GDF11 gene was cloned by RT-PCR and the expression patterns of GDF11 gene were detected. Sequence analysis indicates that the sequences of porcine GDF11 gene were 1161 bp in length(GenBank: HQ657211. 2). The cloned cDNA encoded a protein of 332 amino acid residues, which shared 99. 10%, 99. 10%, 99. 10%, 99. 40%, 99. 10%, 98. 80% and 78. 82% identity with those of human, mouse, rat, cattle, horse, rabbit and zebra fish, respectively. Expression patterns of GDF11 were detected in 14 different tissues by real time quantitative RT-PCR, and patterns of GDF11 gene, as a whole, tended to be spinal cord>backfat>urinary bladder>lung>spleen>gallbladder>kidney>large intestine>heart>skeletal muscle>liver>small intestine>longissimus Dorsi muscle>stomach. These results provide important information for further elucidating the molecular biology functions and mechanism of GDF11 gene.

Key words: Licha black pig, GDF11 gene, Gene cloning, Real time quantitative RT-PCR, Tissue expression patterns

中图分类号:

S828
Q789

邢晋祎, 戈新, 贾坤航, 王建华, 刘忠琛, 李培培, 张宝珣. 里岔黑猪GDF11基因cDNA的克隆及组织表达谱分析[J]. 华北农学报, 2012, 27(1): 24-29. doi: 10.3969/j.issn.1000-7091.2012.01.005.

XING Jin-yi, GE Xin, JIA Kun-hang, WANG Jian-hua, LIU Zhong-chen, LI Pei-pei, ZHANG Bao-xun. Molecular Cloning and Expression Patterns of GDF11 Gene in Licha Black Pig[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(1): 24-29. doi: 10.3969/j.issn.1000-7091.2012.01.005.

http://www.hbnxb.net/CN/Y2012/V27/I1/24

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里岔黑猪GDF11基因cDNA的克隆及组织表达谱分析

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里岔黑猪GDF11基因cDNA的克隆及组织表达谱分析

Molecular Cloning and Expression Patterns of GDF11 Gene in Licha Black Pig

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