华北农学报 ›› 2012, Vol. 27 ›› Issue (1): 106-109. doi: 10.3969/j.issn.1000-7091.2012.01.020

所属专题: 生物技术

• 论文 • 上一篇    下一篇

甜菜雄性核不育基因的SRAP标记

伊六喜1, 斯钦巴特尔1, 孟显光2, 赵瑞哗2, 张惠忠2, 田自华1   

  1. 1. 内蒙古农业大学农学院, 内蒙古呼和浩特 010019;
    2. 内蒙古农牧业科学院, 内蒙古呼和浩特 010031
  • 收稿日期:2011-11-05 出版日期:2012-02-28
  • 通讯作者: 田自华(1960- ), 男, 内蒙古卓资人, 教授, 主要从事甜菜发育生理研究。
  • 作者简介:伊六喜(1986- ), 男, 内蒙古兴安盟人, 在读硕士, 主要从事甜菜发育生理研究。
  • 基金资助:
    国家甜菜产业技术体系重点项目(CARS-210402)

A Study on SRAP Markers for Nucleus Male Sterile Genes in Sugar Beet

YI Liu-xi1, SI Qin-ba-teer1, MENG Xian-guang2, ZHAO Rui-hua2, ZHANG Hui-zhong2, TIAN Zi-hua1   

  1. 1. Institute of Agronomy, Inner Mongolia Agriculture University, Huhhot 010019, China;
    2. Inner Mongolia Academy of Agriculture and Husbandry Sciences, Huhhot 010031, China
  • Received:2011-11-05 Published:2012-02-28

摘要: 采用SRAP技术对甜菜核雄性不育基因进行分子标记的研究,为甜菜育种提供了一条新的途径。以遗传背景相似的可育株和不育株甜菜为材料,用64对SRAP引物组,进行了SRAP分子标记研究。在64对引物组中有2对引物组(即M2E7和M8E8) 可分别得到1个育性基因相关的SRAP分子标记,即M2E7-480和M8E8-130,出现频率分别为73%和76%。表明该SRAP标记技术可用于甜菜辅助育种及遗传多样性分析,从而为甜菜雄性不育相关基因的表达提供科学依据。

关键词: 甜菜, 雄性核不育, SRAP标记

Abstract: A study on SRAP molecular markers for nuclear male sterile genes in sugarbeet was carried out, using male sterile plants and fertile plants with similar genetic background. Results: two pairs of SRAP primers, namely M2E7 and M8E8, produced stable differential PCR bands with frequency of 73% and 76% respectively, which are related to fertility gene. Conclution: this SRAP technque could be used in sugarbeet breeding, genetic diversity studies and research on genes related to fertility in sugarbeet.

Key words: Sugar beet, Nuclear male sterility, SRAP markers

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引用本文

伊六喜, 斯钦巴特尔, 孟显光, 赵瑞哗, 张惠忠, 田自华. 甜菜雄性核不育基因的SRAP标记[J]. 华北农学报, 2012, 27(1): 106-109. doi: 10.3969/j.issn.1000-7091.2012.01.020.

YI Liu-xi, SI Qin-ba-teer, MENG Xian-guang, ZHAO Rui-hua, ZHANG Hui-zhong, TIAN Zi-hua. A Study on SRAP Markers for Nucleus Male Sterile Genes in Sugar Beet[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(1): 106-109. doi: 10.3969/j.issn.1000-7091.2012.01.020.

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