华北农学报 ›› 2011, Vol. 26 ›› Issue (6): 212-216. doi: 10.7668/hbnxb.2011.06.040

• 论文 • 上一篇    下一篇

不同地理来源腐烂茎线虫种群杂交后代致病力测定

王宏宝1,3, 毛佳2, 李茹1, 赵桂东1, 林茂松3   

  1. 1. 江苏徐淮地区淮阴农业科学研究所, 江苏淮安 223001;
    2. 南京农业大学园艺学院, 江苏南京 210095;
    3. 南京农业大学植保学院, 江苏南京 210095
  • 收稿日期:2011-09-20 出版日期:2011-12-28
  • 通讯作者: 林茂松(1948-),男,江苏徐州人,教授,主要从事植物病原线虫学研究。
  • 作者简介:王宏宝(1982-),男,山西大同人,硕士研究生,主要从事植物病原线虫分类学及生物防治工作。
  • 基金资助:
    江苏省农业自主创新项目资助(cx(10)454);江苏淮安市农业科学研究院院长基金

The Pathogenicity Study of Hybrids Populations of Ditylenchus destructor from Different Geographical Origin

WANG Hong-bao1,3, MAO Jia2, LI Ru1, ZHAO Gui-dong1, LIN Mao-song3   

  1. 1. Institute of Agricultural Science of Huaiyin Xuhuai District of Jiangsu, Huaian 223001, China;
    2. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;
    3. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2011-09-20 Published:2011-12-28

摘要: 利用室内人工接种方法对腐烂茎线虫杂交后代进行致病力、繁殖力的测定,结果表明:同一基因型内各组合中均能杂交成功,比较发现各种群的后代数目与对照亲本的数目有显著差异,各杂交组合中杂交率与F1数量均低于对照组(对照组为亲本自交);各杂交群体在致病力、繁殖力表现方面与对照DeSD种群在P0.05水平上均存在差异;各种群正反交群体致病力、繁殖力与对照相比,对照DeSD种群所在的反交组合表现特征均高于其正交组合;相关性分析,腐烂茎线虫两类基因型各种群基因型内杂交群体致病力与繁殖力均存在正相关。本研究对模拟我国不同地理来源腐烂茎线虫种群扩散后所产生杂交代的致病力分化情况,腐烂茎线虫遗传多样性具有重要的应用价值,并为不同地理来源有害生物入侵分险评估提供理论依据。

关键词: 生物学杂交, 腐烂茎线虫, 致病力, 繁殖力

Abstract: Using artificial inoculation methods,on offspring of Ditylenchus destructor, the pathogenicity, fecundity of that were determination, the results showed that: hybrids combinations within the same genotype can successfully. Compared of the number of descendants, found in the various groups and control parents were significantly different, the hybridization rate and the number of F1hybrid were lower than the control group(the control group as the parents selfed) ; The hybrid groups and control DeSD population, in pathogenicity, reproductive performance, there were differences at the P0. 05 level; Compared with groups of reciprocal groups and the control population in the pathogenicity, fecundity,performance characteristics of the anti-crosses was higher than the orthogonal on the control DeSD; Correlation analysis was showed that pathogenicity and fecundity of Ditylenchus destructorhybridization groups were positively correlated. This study has important applications on the genetic diversity of Ditylenchus destructorand the virulence differentiation of simulating proliferation of hybrids of Ditylenchus destructorpopulations in different geographical origin,at the same time,This was provided a theoretical basis on the risk assessment of pest invasion from different geographical origin.

Key words: Biological hybrid, Ditylenchus destructor, Pathogenicity, Fecundity

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引用本文

王宏宝, 毛佳, 李茹, 赵桂东, 林茂松. 不同地理来源腐烂茎线虫种群杂交后代致病力测定[J]. 华北农学报, 2011, 26(6): 212-216. doi: 10.7668/hbnxb.2011.06.040.

WANG Hong-bao, MAO Jia, LI Ru, ZHAO Gui-dong, LIN Mao-song. The Pathogenicity Study of Hybrids Populations of Ditylenchus destructor from Different Geographical Origin[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 212-216. doi: 10.7668/hbnxb.2011.06.040.

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