摘要： 磷脂酶C(Phospholipase C,PLC)通过降解细胞膜上的磷脂分子产生IP3和DAG两个第二信使分子,分别激活下游的Ca2+途径和蛋白激酶C。研究表明,PLC与植物病原真菌的致病及发育过程有关。利用PLC特异性抑制剂U-73122初步研究了玉米大斑病菌PLC的功能。结果显示,U-73122对玉米大斑病菌菌丝生长及分生孢子的萌发均具有明显的抑制作用,且抑制率随抑制剂浓度升高而增强;并且可引起芽管的缩短和不规则膨大。此外,U-73122也可以导致病菌粗毒素的生物学活性明显降低。由此说明PLC可以通过影响菌丝及芽管的极性生长,而调控玉米大斑病菌的菌丝及分生孢子发育;同时参与病菌的毒素合成。
关键词:
玉米大斑病菌,
磷脂酶C,
U-73122,
分生孢子,
毒素
Abstract: Phospholipase C(PLC)could catalyze the hydrolysis of phospholipid molecules on cell membrane and generate two second messenger molecules IP3 and DAG, which activated Ca“signal pathway and protein kinase C re— spectively.It had been proved that PLC modulated pathogenicity and development of plant pathogenic fungi.In this research, U-73 1 22, the specific inhibitor of PLCs, was used to study functions of PLCs in Setosphaeria turcica prelimina— ry.U一73122 showed significant inhibition effects on hyphal growth and conidial germination.The higher was the inhibitor concentration, the lower was the germination ratio of spores.U-73 122 caused the germ tube shortening and cell swelling irregularly.In addition, U一73 1 22 could also obviously decrease the biological activity of HT-toxin.It was indi— cated that PLC might regulate hyphal growth and conidial germination by controlling the cell polarity, and also involved in the biosynthesis of HT—toxin in Setosphaeria turcica.
Key words:
Setosphaeria turcica,
Phospholipase C,
U·73 122,
Conidia,
HT-toxin
中图分类号:
郝志敏, 郝杰, 李志勇, 董金皋. 磷脂酶C特异性抑制剂U-73122对玉米大斑病菌分生孢子萌发及毒素活性的影响[J]. 华北农学报, 2010, 25(6): 118-121. doi: 10.7668/hbnxb.2010.06.022.
HAO Zhi-min, HAO Jie, LI Zhi-yong, DONG Jin-gao. Effects of Phospholipase C Inhibitor U-73122 on Conidial Germination and HT-toxin Activity of Setosphaeria turcica[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 118-121. doi: 10.7668/hbnxb.2010.06.022.