摘要： 以小麦成熟胚为转化受体,采用农杆菌介导的遗传转化技术,建立了小麦的转基因株系。结果表明,建立的小麦遗传转化和植株再生体系,在选择压下由侵染的小麦成熟胚中,获得的抗性愈伤组织频率为57.50%,愈伤组织分化频率为8.58%,植株分化比例为0.83%。对再生植株中报告基因Gus的PCR检测结果表明,供试3株小麦的PCR均为阳性。与未进行遗传转化的对照植株相比,再生植株的Gus活性明显提高。表明再生的供试植株均为转基因植株。因此,本研究建立的小麦成熟胚遗传转化和再生体系,为今后小麦的高频转基因系的获得提供了参考依据。

关键词: 小麦(Triticum aestivum L.), 成熟胚, 遗传转化, 分子鉴定

Abstract: In this study,the genetic transformation procedure of wheat mediated via Agrobacterium -tumefaciens in which the mature embryos to be the explants was established.Under selection pressure condition,the generation rate of resistant calli to the transformed total mature embryos was 57.50 %,with a differentiation rate of resistant buds to the transformed total mature embryos being 8.58 %, and a plant generation rate of generated plants to the transformed total mature embryos being 0.83 %.In the tested three generated plants,PCR amplifications for the reporter gene Gus were all positive.In the meantime,the Gus activities in the plants were all significantly higher than the control (non -transformed plants).These results demonstrated that the tested plants generated from the transformed mature embryos were transgenic. Therefore,the genetic transformation approach established in this study would provide a powerful method for establishment of transgenic wheat lines in the future.

Key words: Wheat(TriticumaestivumL.), Matureembryos, Genetictransformation, Molecularidentification

中图分类号: 

• S512.03