摘要： 以柞蚕核型多角体病毒(ApNPV)DNA为模板，运用PCR技术钓取ApNPV(IE-1)基因的启动子片段，并将其克隆到pMD18-T载体上进行测序分析。将测序的2.7 kb DNA片段用EcoR I和Bgl II双酶切，回收3’端1.6 kb的DNA片段。将该片段插入到pGFP-N₂表达载体上构建以其作为启动子的IE-1/pGFP-N₂重组质粒。重组质粒经脂质体介导转染Hela细胞，结果可见绿色荧光蛋白(GFP)在细胞中的表达，证明钓取的IE-1基因启动子片段具有启动子的转录活性。

关键词: IE-1基因启动子, 柞蚕核型多角体病毒, pGFP-N2表达载体

Abstract: A 2.7 kb fragment of IE promoter(IE-1)was got from ApNPV by PCR and subcloned into pMD18-T and sequenced.An expression vector ofIE-1/pGFP-N2 was constructed after 1.6 kb fragment of 3' flanking region of IE promoter was inserted into pGFP-N2 atEcoRI andBglII sites.GFP was expressed in Hela cells after transfected,which proved thatIE-1can active the expression of foreign gene.

Key words: IE promoter, ApNPV, pGFP-N2 expression vector

中图分类号: 

• S882