夹竹桃苯丙氨酸解氨酶的基因克隆与序列分析

doi:10.7668/hbnxb.2007.04.005

华北农学报 ›› 2007, Vol. 22 ›› Issue (4): 19-24. doi: 10.7668/hbnxb.2007.04.005

所属专题： 生物技术；

夹竹桃苯丙氨酸解氨酶的基因克隆与序列分析

王燕¹, 许锋¹, 杜何为², 蔡荣¹, 陈柳吉¹, 程水源^1,3

1. 长江大学园艺园林学院, 湖北荆州 434025;
2. 长江大学生命科学学院, 湖北荆州 434025;
3. 黄冈师范学院生命科学与工程学院, 湖北黄冈 438000

收稿日期:2007-05-23 出版日期:2007-08-28
通讯作者: 程水源(1965-)，男，湖北天门人，教授，博士生导师，主要从事园艺植物育种与分子生物学方面的研究。
作者简介:王燕(1967-)，女，江苏南通人，副教授，主要从事园艺植物育种与分子生物学方面的研究。
基金资助:
教育部新世纪优秀人才计划(NCET-04-0746);教育部攻关项目(02095);湖北省自然科学基金(2002AB094);湖北省青年杰出人才基金(2003AB014);湖北省教育厅重大科技项目(Z200627002)

Molecular Cloning and Sequence Analysis of a Phenylalanine Ammonia-lyase cDNA from Nerium oleander

WANG Yan¹, XU Feng¹, DU He-wei², CAI Rong¹, CHEN Liu-ji¹, CHENG Shui-yuan^1,3

1. College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China;
2. College of Life Science, Yangtze University, Jingzhou 434025, China;
3. College of Life Science and Engineering, Huanggang Normal University, Huanggang 438000, China

Received:2007-05-23 Published:2007-08-28

摘要/Abstract

摘要： 首次从夹竹桃中克隆得到苯丙氨酸解氨酶(PAL)基因cDNA片段，并命名为NoPAL1，GenBank登录号为AY74777，这也是首次从夹竹桃花青苷代谢途径中克隆得到的基因。NoPAL1长8个bp，编码289个氨基酸。通过核苷酸和蛋白质序列多重比较发现NoPAL1与其他植物的PAL基因高度同源。NoPAL1编码的蛋白质序列包含与水稻、玉米PAL蛋白质相同的脱氨基位点和催化活性位点。PAL系统进化树表明，NoPAL1与乔木类植物的PAL基因聚类关系最近。克隆NoPAL1基因为利用基因工程技术来调控夹竹桃花青苷的代谢从而调控夹竹桃花的颜色提供了基因资源。

关键词: NoPAL1, 苯丙氨酸解氨酶, 夹竹桃, 基因克隆, 序列分析

Abstract: A phenylalanine ammonia-lyase (PAL) gene was clone from Nerium oleander for the first time and it was also the first cloned gene involved in anthocyanin metabolic pathway in N. oleander. The PAL cDNA fragment of N. oleander (designated as NoPAL1) was 866bp and it encoded a 289 amino acid protein. NoPAL1 was found to have extensive homology with those of other plant PAL genes via multiple alignments. The domination sites and catalytic active sites in PAL protein of Oryza sativa and Zea mays were also found in NoPAL1. Phylogenetic tree analysis revealed that NoPAL1 had closer relationship with PALs from arbor plants than from other plants. NoPAL1 is a useful tool to study the regulation of anthocyanis metabolism in N. oleander.

Key words: NoPAL1, PAL, Nerium oleander, Molecular cloning, Sequence analysis

中图分类号:

S662.103

王燕, 许锋, 杜何为, 蔡荣, 陈柳吉, 程水源. 夹竹桃苯丙氨酸解氨酶的基因克隆与序列分析[J]. 华北农学报, 2007, 22(4): 19-24. doi: 10.7668/hbnxb.2007.04.005.

WANG Yan, XU Feng, DU He-wei, CAI Rong, CHEN Liu-ji, CHENG Shui-yuan. Molecular Cloning and Sequence Analysis of a Phenylalanine Ammonia-lyase cDNA from Nerium oleander[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 19-24. doi: 10.7668/hbnxb.2007.04.005.

http://www.hbnxb.net/CN/Y2007/V22/I4/19

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夹竹桃苯丙氨酸解氨酶的基因克隆与序列分析

Molecular Cloning and Sequence Analysis of a Phenylalanine Ammonia-lyase cDNA from Nerium oleander

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