华北农学报 ›› 2006, Vol. 21 ›› Issue (4): 27-30. doi: 10.3321/j.issn:1000-7091.2006.04.007

所属专题: 黄瓜 蔬菜专题

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利用抗微管除草剂胺磺灵诱导黄瓜四倍体

韩毅科, 杜胜利, 张桂华, 魏爱民   

  1. 天津科润黄瓜研究所, 天津 300192
  • 收稿日期:2005-12-02 出版日期:2006-08-28
  • 作者简介:韩毅科(1968- ),男,河南商水人,助理研究员,硕士,主要从事生物技术与蔬菜遗传育种研究工作。
  • 基金资助:
    天津市农科院院长基金(03014)

Anti-microtubule Herbicides Oryzalin for Induction of Tetraploid Cucumber

HAN Yi-ke, DU Sheng-li, ZHANG Gui-hua, WEI Ai-min   

  1. Tianjin Kerun Cucumber Research Institute, Tianjin 300192, China
  • Received:2005-12-02 Published:2006-08-28

摘要: 用Oryzalin对黄瓜茎尖进行四倍体诱导试验,结果表明,浓度为100~200μmol/L Oryzalin处理24 h对黄瓜茎尖离体四倍体诱导较为合适,可以有效代替秋水仙素。和秋水仙素相比,Oryzalin使用的浓度低,效果好,且毒性小,对人和环境比较安全,为诱导黄瓜染色体加倍的理想诱导剂。并用流式细胞仪对黄瓜离体诱导的幼苗进行了倍性分析试验。

关键词: 黄瓜, 胺磺灵, 四倍体, 染色体加倍

Abstract: The cucumber shoot apices were treated with different concentration of oryzalin or colchiine to induce tetraploid plantlet.The results showed that oryzalin was an efficient agent for induction of tetraploid cucumber with shoot apices,the suitable concentrations range was 100-200 μmol/L with 24 h treatment.Colchine could be efficiently replaced by oryzalin.Oryzalin concentration for tetraploid induction is very low and effective.It is low toxicity and less harmful to human and environment.Compared to colchicines,it is an ideal induction agent for cucumber chromosome doubling.Flow cytometry was used to determine the ploidy of cucumber plantlets induced after oryzalin and colchine treatment in vitro.

Key words: Cucumber, Oryzalin, Tetraploid, Chromosome doubling

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引用本文

韩毅科, 杜胜利, 张桂华, 魏爱民. 利用抗微管除草剂胺磺灵诱导黄瓜四倍体[J]. 华北农学报, 2006, 21(4): 27-30. doi: 10.3321/j.issn:1000-7091.2006.04.007.

HAN Yi-ke, DU Sheng-li, ZHANG Gui-hua, WEI Ai-min. Anti-microtubule Herbicides Oryzalin for Induction of Tetraploid Cucumber[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(4): 27-30. doi: 10.3321/j.issn:1000-7091.2006.04.007.