摘要： 以植酸钙作为唯一的磷源,从无花果中分离到一株产植酸酶的菌株,利用液体发酵对该菌株的发酵工艺进行研究,获得最适发酵培养基即淀粉8%,葡萄糖2%,NaNO3 1.0%,KH2PO4 0.01%,KCl 0.05%,MgSO4 0.05%,FeSO4 0.01%,pH值为5.0,采用10%的接种量,10%的装液量,经30℃,150 r/min 摇瓶培养8d,发酵液酶活达870.87pu/mL;采用该酶处理豆粕粉30℃下反应90min,植酸(盐)降解率达75 %,处理液中可溶性蛋白质含量由0.167mg/mL增加到12.1mg/mL。
关键词:
植酸酶,
植酸钙培养基,
液体深层发酵
Abstract: With phytate-Ca as the sole phosphorus source of selected medium,a extracellular phytase-producing strain was screened from the ficuum.Liquid fermentation was studied and a best fermentation medium was obtained,starch 8%,glucose 2%,NaNO3 1.0%,KH2PO4 0.01%,KCl 0.05%,MgSO4 0.05%,FeSO4 0.01% and initial pH 5.0.Inoculating amount 10%,filling liquid volume 10%,extracellular phytase production as much as 870.87 pu/mL fermentation liquid was obtained at 30℃ after 8 days.More 75% phytic acid of soybean was decomposed by the fermenting liquid at 30℃ for 90 min,and soluble protein content in treating liquid was increased from 0.167 mg/mL to 12.1 mg/mL.
Key words:
Phytase,
Phytate-Ca medium,
Liquid fermentation
中图分类号:
张梅申, 沈爱光, 熊晓辉, 沈昌. 植酸酶产生菌株的液体深层发酵条件研究[J]. 华北农学报, 1999, 14(4): 63-67. doi: 10.3321/j.issn:1000-7091.1999.04.013.
Zhang Meishen, Shen Aiguang, Xiong Xiaohui, Shen Chang. A Study on Liquid Fermentation of Phytase-production Strain[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1999, 14(4): 63-67. doi: 10.3321/j.issn:1000-7091.1999.04.013.