东北林蛙<i>LEPR</i>基因的克隆及其在感染中的表达分析

doi:10.7668/hbnxb.20193754

摘要/Abstract

摘要：

为了探究LEPR基因在两栖类的生物学功能,利用嗜水气单胞菌感染东北林蛙建立炎症模型,分析LEPR基因在细菌感染后的表达情况。首先利用RT-PCR技术克隆LEPR基因并进行生物信息学分析,再构建Ah感染的东北林蛙炎症模型;通过苏木精-伊红染色法(HE染色)观察感染后组织病理变化,并利用qRT-PCR技术分析生理状态和感染状态LEPR基因的组织表达规律差异,最后结合免疫组织化学染色对肾脏、皮肤和肌肉等组织中LEPR蛋白表达变化进行检测。结果显示,获得东北林蛙LEPR基因序列长度为3 604 bp,其开放读码框为3 405 bp,共编码1 134个氨基酸;亚细胞定位显示,LEPR蛋白质为具有一次跨膜结构域的膜蛋白;同源性分析证实东北林蛙与两栖类同源性在52.6%以上,表明LEPR的保守程度较低;基于qRT-PCR结果显示,LEPR mRNA在健康东北林蛙心脏、肝脏、脾脏、肺脏、肾脏、皮肤、肌肉和胃等8种组织中均有表达,在皮肤组织中的相对表达量显著高于其他组织;Ah感染后LEPR基因在不同组织中显著上调,但应答时间和水平有所差异;免疫组织化学结果表明,肾脏、皮肤和肌肉LEPR蛋白表达量变化趋势与qRT-PCR结果基本一致。皮肤组织中LEPR基因应答强烈,也说明其可能参与感染过程,为进一步扩展两栖类LEPR基因的免疫学功能研究奠定了基础。

关键词: 东北林蛙, 嗜水气单胞菌, LEPR基因, 组织表达

Abstract:

In order to explore the biological function of LEPR gene in amphibians,we infected Rana dybowskii with Aeromonas hydrophila(Ah)to establish an inflammatory model,and analyzed expression profile of LEPR gene after Ah infection.Firstly,the LEPR gene was cloned by RT-PCR technology and bioinformatics analysis was performed,and then the inflammation model of Rana dybowskii was constructed.The histopathological changes during infection were observed by hematoxylin eosin staining(HE staining),and the differences of tissue expression of LEPR gene in physiological and infectious states were analyzed by qRT-PCR.Finally,the changes of LEPR protein expression in kidney,skin and muscle tissues were verified by immunohistochemistry staining.The results showed that the length of LEPR gene sequence of Rana dybowskii was 3 604 bp,and its open reading frame was 3 405 bp,encoding 1 134 amino acids.Subcellular localization showed that the LEPR protein was a membrane protein with a primary transmembrane domain.The homology analysis confirmed that the homology between Rana dybowskii and amphibians was more than 52.6%,indicating that LEPR was less conservative.Based on qRT-PCR results,LEPR mRNA was expressed in the heart,liver,spleen,lung,kidney,skin,muscle and stomach of the healthy Rana dybowskii,and its relative expression in skin tissue was significantly higher than that in other tissues.After Ah infection,the LEPR gene was significantly up-regulated in different tissues,but the response time and level were different.Immunohistochemical results showed that the change trend of LEPR protein expression in kidney,skin and muscle was basically consistent with that of qRT-PCR.The strong response of the LEPR gene in the skin tissue also indicates that it may participate in the infection process,which lays a foundation for further expanding the immunological function of the amphibian LEPR gene.

Key words: Rana dybowskii, Aeromonas hydrophila, LEPR gene, Tissue expression

许冬梅, 刘婷婷, 刘依铭, 刘玉芬, 刘鹏, 赵文阁. 东北林蛙LEPR基因的克隆及其在感染中的表达分析[J]. 华北农学报, 2023, 38(3): 227-238. doi: 10.7668/hbnxb.20193754.

XU Dongmei, LIU Tingting, LIU Yiming, LIU Yufen, LIU Peng, ZHAO Wenge. Cloning and Expression Analysis of Rana dybowskii LEPR Gene During Infection[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(3): 227-238. doi: 10.7668/hbnxb.20193754.

http://www.hbnxb.net/CN/Y2023/V38/I3/227

图/表 12

表1 本研究所用引物

Tab.1 Primers used in this study

基因 Gene	引物序列(5'-3') Primer sequences (5'-3')	退火温度/℃ Tm	产物长度/bp Product size	目的 Purpose
LEPR-A	F:ACACTCGCCAAACACTCA	47.3	969	RT-PCR
	R:AACGCACTTCAAACACCAG
LEPR-B	F:CGCTGGTGTTTGAAGTGC	53.8	683	RT-PCR
	R:GGGAATGACGCAGACAGG
LEPR-C	F:GAGGACGGATTTTACAGATG	54.5	1 252	RT-PCR
	R:AGGATTAAGGGGAAACTGGG
LEPR-D	F:ATGAGATGCAGAATAATG	51.5	1 099	RT-PCR
	R:ACTATCTTTGCAGCAAAC
LEPR-q	F:GGGAATGACGCAGACAGG	52.0	121	qRT-PCR
	R:GGAGGACGGATTTTACAGAT
β-actin	F:AAGAATGAGGGCTGGAACA	52.0	176	qRT-PCR
	R:GTGCGTGACATCAAGGAGAAGC

图1 东北林蛙 LEPR 基因的克隆 M.DL2000 DNA Marker。

Fig.1 Cloning of LEPR gene in Rana dybowskii

图2 东北林蛙LEPR蛋白的理化性质 A.LEPR蛋白的跨膜结构域;B.LEPR蛋白磷酸化位点预测;C.LEPR蛋白亲水性/疏水性分析;D.LEPR蛋白功能结构域预测。

Fig.2 Physicochemical properties of LEPR protein in Rana dybowskii A.The transmembrane domain of LEPR protein;B.Prediction of LEPR protein phosphorylation sites;C.Hydrophilic/hydrophobic analysis of LEPR protein;D.Prediction of LEPR protein functional domain.

图3 蛋白质二级、三级结构及蛋白互作网络 A.LEPR蛋白质二级结构;B.LEPR蛋白质三级结构;C.LEPR蛋白质蛋白互作网络。

Fig.3 Protein secondary and tertiary structures and protein interaction networks A.LEPR protein secondary structure;B.LEPR protein tertiary structure;C.LEPR protein interaction network.

图4 东北林蛙LEPR同源性分析和氨基酸系统进化树的构建 A.LEPR基因同源性;B.LEPR系统进化树。

Fig.4 Homology analysis from LEPR and construction of amino acid phylogenetic tree of Rana dybowskii A.LEPR gene homology;B.LEPR phylogenetic tree.

图5 嗜水气单胞菌感染后东北林蛙病理组织切片 HE染色观察嗜水气单胞菌感染的东北林蛙的不同组织。A.肝脏;B.皮肤;1,2,3.0,36,72 h。

Fig.5 Histopathological section of Rana dybowskii challenged with Ah Observation by HE staining in different tissues of Rana dybowskii infected by Ah.A.Liver;B.Skin;1,2,3.0,36,72 h.

图6 LEPR 基因在东北林蛙的不同组织中的表达水平不同小写字母表示在不同组织中的相对表达量差异性显著(P<0.05)。

Fig.6 Expression level of LEPR gene in different tissues of Rana dybowskii Different lowercase letters indicate significant differences in relative expression among tissues(P<0.05).

图7 嗜水气单胞菌感染东北林蛙LEPR基因的相对表达量变化 A.心脏;B.肝脏;C.脾脏;D.肺脏;E.肾脏;F.皮肤;G.肌肉;H.胃。不同小写字母表示 5%水平差异显著(P<0.05)。

Fig.7 Changes of relative expression of LEPR gene in Rana dybowskii infected by Ah A.Heart;B.Liver;C.Spleen;D.Lung;E.Kidney;F.Skin;G.Muscle;H.Stomach.Different lowercase letters indicate significantly different at 5% level(P<0.05).

图8 嗜水气单胞菌感染后不同时间点肾脏的免疫组织化学染色 A-G分别表示对照组、感染后8,16,24,36,48,72 h肾脏组织。

Fig.8 Immunohistochemical staining of kidney at different time points after Ah infection A-G respectively represent the kidney tissue in the control group,8,16,24,36,48,72 h after infection.

图9 嗜水气单胞菌感染后不同时间点皮肤的免疫组织化学染色 A-G分别表示对照组、感染后8,16,24,36,48,72 h皮肤组织。

Fig.9 Immunohistochemical staining of skin at different time points after Ah infection A-G respectively represent the skin tissue in the control group,8,16,24,36,48,72 h after infection.

图10 嗜水气单胞菌感染后不同时间点肌肉的免疫组织化学染色 A-G分别表示对照组、感染后8,16,24,36,48,72 h肌肉组织。

Fig.10 Immunohistochemical staining of muscle at different time points after Ah infection A-G respectively represent the muscle tissue in the control group,8,16,24,36,48,72 h after infection.

图11 东北林蛙嗜水气单胞菌感染下肾脏、皮肤和肌肉的LEPR差异表达分析不同大写字母表示1%水平差异显著(P<0.01)。

Fig.11 Differential expression of LEPR in kidney,skin and muscle of Rana dybowskii infected with Ah Different capital letters are significantly at 1% level(P<0.01).

参考文献 45

[1]	Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman J M. Positional cloning of the mouse obese gene and its human homologue[J]. Nature, 1994, 372(6505):425-432.doi:10.1038/372425a0. doi: 10.1038/372425a0
[2]	Karakoç Z, Topalogˇlu U, Bayram B. Immunohistochemical distributions of leptin,ghrelin and obestatin hormones in bull and ram abomasum[J]. Anatomia Histologia Embryologia, 2022, 51(3):411-418.doi:10.1111/ahe.12801. doi: 10.1111/ahe.12801 URL
[3]	Tsakoumis E, Ahi E P, Schmitz M. Impaired leptin signaling causes subfertility in female zebrafish[J]. Molecular and Cellular Endocrinology, 2022, 546:111595.doi:10.1016/j.mce.2022.111595. doi: 10.1016/j.mce.2022.111595 URL
[4]	Kamstra K, Rizwan M Z, Grattan D R, Horsfield J A, Tups A. Leptin regulates glucose homeostasis via the canonical Wnt pathway in the zebrafish[J]. FASEB Journal, 2022, 36(3):e22207.doi:10.1096/fj.202101764R. doi: 10.1096/fj.202101764R
[5]	Swain J E, Dunn R L, McConnell D, Gonzalez-Martinez J, Smith G D. Direct effects of leptin on mouse reproductive function:Regulation of follicular,oocyte,and embryo development[J]. Biology of Reproduction, 2004, 71(5):1446-1452.doi:10.1095/biolreprod.104.033035. doi: 10.1095/biolreprod.104.033035 URL
[6]	Friedman J M. The function of leptin in nutrition,weight,and physiology[J]. Nutrition Reviews, 2002, 60(10):S1-S14.doi:10.1301/002966402320634878. doi: 10.1301/002966402320634878
[7]	Ducy P, Amling M, Takeda S, Priemel M, Schilling A F, Beil F T, Shen J, Vinson C, Rueger J M, Karsenty G. Leptin inhibits bone formation through a hypothalamic relay:A central control of bone mass[J]. Cell, 2000, 100(2):197-207.doi:10.1016/s0092-8674(00)81558-5. doi: 10.1016/s0092-8674(00)81558-5 pmid: 10660043
[8]	Guo Z, Yang H Q, Zhang J R, Zeng W W, Hu X Y. Leptin receptor signaling sustains metabolic fitness of alveolar macrophages to attenuate pulmonary inflammation[J]. Science Advances, 2022, 8(28):eabo3064.doi:10.1126/sciadv.abo3064. doi: 10.1126/sciadv.abo3064
[9]	Tian Y M, Tian S Y, Wang D, Cui F, Zhang X J, Zhang Y. Elevated expression of the leptin receptor ob-R may contribute to inflammation in patients with ulcerative colitis[J]. Mol Med Rep, 2019, 20(5):4706-4712.doi:10.3892/mmr.2019.10720. doi: 10.3892/mmr.2019.10720
[10]	Pérez-Pérez A, Sánchez-Jiménez F, Vilariño-García T, Sánchez-Margalet V. URL
[11]	Tartaglia L A, Dembski M, Weng X, Deng N, Culpepper J, Devos R, Richards G J, Campfield L A, Clark F T, Deeds J, Muir C, Sanker S, Moriarty A, Moore K J, Smutko J S, Mays G G, Wool E A, Monroe C A, Tepper R I. Identification and expression cloning of a leptin receptor,OB-R[J]. Cell, 1995, 83(7):1263-1271.doi:10.1016/0092-8674(95)90151-5. doi: 10.1016/0092-8674(95)90151-5 pmid: 8548812
[12]	Schaab M, Kratzsch J. The soluble leptin receptor[J]. Best Practice & Research Clinical Endocrinology & Metabolism, 2015, 29(5):661-670.doi:10.1016/j.beem.2015.08.002. doi: 10.1016/j.beem.2015.08.002
[13]	Wada N, Hirako S, Takenoya F, Kageyama H, Okabe M, Shioda S. Leptin and its receptors[J]. Journal of Chemical Neuroanatomy, 2014, 61/62:191-199.doi:10.1016/j.jchemneu.2014.09.002. doi: 10.1016/j.jchemneu.2014.09.002 URL
[14]	Li Y, Zhou Y, Lei L, Deng X X, Duan Y T, Xu J F, Fu S X, Long R, Yuan D Y, Zhou C W. Molecular cloning and tissue distribution of the leptin gene in gibel carp(Carassius auratus gibelio):Regulation by postprandial and long-term fasting treatment[J]. Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 2022, 266:111156.doi:10.1016/j.cbpa.2022.111156. doi: 10.1016/j.cbpa.2022.111156
[15]	Liu X, Dunn I C, Sharp P J, Boswell T. Molecular cloning and tissue distribution of a short form chicken leptin receptor mRNA[J]. Domest Anim Endocrinol, 2007, 32(3):155-166.doi:10.1016/j.domaniend.2006.02.001. doi: 10.1016/j.domaniend.2006.02.001 URL
[16]	Villanueva E C, Myers M G Jr. Leptin receptor signaling and the regulation of mammalian physiology[J]. International Journal of Obesity, 2008, 32(S7):S8-S12.doi:10.1038/ijo.2008.232. doi: 10.1038/ijo.2008.232
[17]	Kolić I, Stojković L, Dincˇić E, Jovanović I, Stanković A, Živković M. Expression of LEP, LEPR and PGC1A genes is altered in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis[J]. Journal of Neuroimmunology, 2020, 338:577090.doi:10.1016/j.jneuroim.2019.577090. doi: 10.1016/j.jneuroim.2019.577090 URL
[18]	Procaccini C, Jirillo E, Matarese G. Leptin as an immunomodulator[J]. Molecular Aspects of Medicine, 2012, 33(1):35-45.doi:10.1016/j.mam.2011.10.012. doi: 10.1016/j.mam.2011.10.012 pmid: 22040697
[19]	Crespi E J, Denver R J. Leptin(ob gene)of the South African clawed frog Xenopus laevis[J]. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(26):10092-10097.doi:10.1073/pnas.0507519103. doi: 10.1073/pnas.0507519103 pmid: 16782821
[20]	赵文阁, 刘鹏, 陈辉. 黑龙江省两栖爬行动物志[M]. 北京: 科学出版社, 2008.
	Zhao W G, Liu P, Chen H. Amphibians and reptiles in Heilongjiang Province[M]. Beijing: Science Press, 2008.
[21]	Chen X Y, Ren C L, Teng Y R, Shen Y J, Wu M Y, Xiao H, Wang H Y. Effects of temperature on growth,development and the leptin signaling pathway of Bufo gargarizans[J]. Journal of Thermal Biology, 2021, 96:102822.doi:10.1016/j.jtherbio.2020.102822. doi: 10.1016/j.jtherbio.2020.102822 URL
[22]	郭欣奕. Leptin及其受体基因与峨眉树蛙抱对模式相关性研究[D]. 武汉: 华中师范大学, 2015.
	Guo X Y. Study on the correlation between Leptin and leptin receptor gene and embracing pattern of tree frog Emei[D]. Wuhan: Central China Normal University, 2015.
[23]	张傲. 东北林蛙油中糖和脂类及其代谢相关因子表达的研究[D]. 北京: 北京林业大学, 2020.
	doi:10.26949/d.cnki.gblyu.2020. 001458. Zhang A.Study on the expression of sugar,lipid and their metabolic related factors in Rana chensinensis oil[D]. Beijing: Beijing Forestry University, 2020. doi: 10.26949/d.cnki.gblyu.2020
[24]	蒋霞. 嗜水气单胞菌致病性研究进展[J]. 生物技术世界, 2014, 11(6):75,77.
	Jiang X. Research progress on pathogenicity of Aeromonas hydrophila[J]. Biotech World, 2014, 11(6):75,77.
[25]	许晴. 嗜水气单胞菌胁迫对东北林蛙血细胞及白介素IL-1β和IL-10表达的影响[D]. 哈尔滨: 东北林业大学, 2021.doi:10.27009/d.cnki.gdblu.2021.000626. doi: 10.27009/d.cnki.gdblu.2021.000626
	Xu Q. Effects of Aeromonas hydrophila stress on the expression of blood cells and IL-1β and IL-10 in Rana chensinensis[D]. Harbin:Northeast Forestry University, 2021.
[26]	吴彤, 刘婷婷, 刘依铭, 赵雪滢, 刘玉芬, 刘鹏, 赵文阁. 东北林蛙瘦素受体叠加转录蛋白基因的克隆及组织表达分析[J]. 生物工程学报, 2022, 38(5):1859-1873.doi:10.13345/j.cjb.210615. doi: 10.13345/j.cjb.210615
	Wu T, Liu T T, Liu Y M, Zhao X Y, Liu Y F, Liu P, Zhao W G. Cloning and tissue expression analysis of the LepROT gene of Rana dybowskii[J]. Chinese Journal of Biotechnology, 2022, 38(5):1859-1873.
[27]	史雪灿, 柴龙会, 牛曙东, 肖向红. 嗜水气单胞菌胁迫下东北林蛙皮肤MyD88和TRAF6基因表达的动态变化[J]. 动物医学进展, 2015, 36(6):59-63.doi:10.3969/j.issn.1007-5038.2015.06.012. doi: 10.3969/j.issn.1007-5038.2015.06.012
	Shi X C, Chai L H, Niu S D, Xiao X H. Expression dynamics of MyD88 and TRAF6 genes in Rana dybowskii infected with Aeromonas hydrophila[J]. Progress in Veterinary Medicine, 2015, 36(6):59-63.
[28]	Liu Q, Chen Y, Copeland D, Ball H, Duff R J, Rockich B, Londraville R L. Expression of leptin receptor gene in developing and adult zebrafish[J]. General and Comparative Endocrinology, 2010, 166(2):346-355.doi:10.1016/j.ygcen.2009.11.015. doi: 10.1016/j.ygcen.2009.11.015 pmid: 19941865
[29]	Zhang H X, Chen H P, Zhang Y, Li S S, Lu D Q, Zhang H F, Meng Z N, Liu X C, Lin H R. Molecular cloning,characterization and expression profiles of multiple leptin genes and a leptin receptor gene in orange-spotted grouper(Epinephelus coioides)[J]. General and Comparative Endocrinology, 2013, 181:295-305.doi:10.1016/j.ygcen.2012.09.008. doi: 10.1016/j.ygcen.2012.09.008 URL
[30]	Rønnestad I, Nilsen T O, Murashita K, Angotzi A R, Gamst Moen A G, Stefansson S O, Kling P, Thrandur Björnsson B, Kurokawa T. Leptin and leptin receptor genes in Atlantic salmon:Cloning,phylogeny,tissue distribution and expression correlated to long-term feeding status[J]. General and Comparative Endocrinology, 2010, 168(1):55-70.doi:10.1016/j.ygcen.2010.04.010. doi: 10.1016/j.ygcen.2010.04.010 pmid: 20403358
[31]	Shpilman M, Hollander-Cohen L, Ventura T, Gertler A, Levavi-Sivan B. Production,gene structure and characterization of two orthologs of leptin and a leptin receptor in tilapia[J]. General and Comparative Endocrinology, 2014, 207:74-85.doi:10.1016/j.ygcen.2014.05.006. doi: 10.1016/j.ygcen.2014.05.006 pmid: 24852346
[32]	Gong N P, Einarsdottir I E, Johansson M, Björnsson B T. Alternative splice variants of the rainbow trout leptin receptor encode multiple circulating leptin-binding proteins[J]. Endocrinology, 2013, 154(7):2331-2340.doi:10.1210/en.2012-2082. doi: 10.1210/en.2012-2082 pmid: 23645152
[33]	Martins F F, Souza-Mello V, Carvalho J J D, Del Sol M, Aguila M B, Mandarim-De-Lacerda C A. Liver structural injury in leptin-deficient(ob/ob)mice:Lipogenesis,fibrogenesis,inflammation and apoptosis[J]. International Journal of Morphology, 2021, 39(3):732-738.doi:10.4067/S0717-95022021000300732. doi: 10.4067/S0717-95022021000300732 URL
[34]	翁晨虹, 张亚洲, 李佑杰, 许燕燕, 周秋白. 黄鳝瘦素和瘦素受体基因克隆及组织分布分析[J]. 江西农业大学学报, 2022, 44(2):443-451.doi:10.13836/j.jjau.2022046. doi: 10.13836/j.jjau.2022046
	Weng C H, Zhang Y Z, Li Y J, Xu Y Y, Zhou Q B. Cloning and tissue distribution analysis of swamp eel(Monopterus albus)leptin and leptin receptor gene[J]. Acta Agriculturae Universitatis Jiangxiensis(Natural Sciences Edition), 2022, 44(2):443-451.
[35]	张冬梅, 赵柳兰, 刘巧, 何阔, 廖磊, 罗杰, 孙俊龙, 杨淞. 大口黑鲈leptin A基因的组织表达及其对急性低氧胁迫的响应[J]. 水产学报, 2020, 44(10):1609-1618.doi:10.11964/jfc.20200112141. doi: 10.11964/jfc.20200112141
	Zhang D M, Zhao L L, Liu Q, He K, Liao L, Luo J, Sun J L, Yang S. Tissue expression of leptin A gene in Micropterus salmoides and its response to acute hypoxia stress[J]. Journal of Fisheries of China, 2020, 44(10):1609-1618.
[36]	Trombley S, Maugars G, Kling P, Björnsson B T, Schmitz M. Effects of long-term restricted feeding on plasma leptin,hepatic leptin expression and leptin receptor expression in juvenile Atlantic salmon(Salmo salar L.)[J]. General and Comparative Endocrinology, 2012, 175(1):92-99.doi:10.1016/j.ygcen.2011.10.001. doi: 10.1016/j.ygcen.2011.10.001 pmid: 22019478
[37]	Escobar S, Rocha A, Felip A, Carrillo M, Zanuy S, Kah O, Servili A. Leptin receptor gene in the European Sea bass(Dicentrarchus labrax):Cloning,phylogeny,tissue distribution and neuroanatomical organization[J]. General and Comparative Endocrinology, 2016, 229:100-111.doi:10.1016/j.ygcen.2016.03.017. doi: 10.1016/j.ygcen.2016.03.017 URL
[38]	Zhou Y, Feng L, Jiang W D, Wu P, Liu Y, Jiang J, Kuang S Y, Tang L, Peng Y, Zhou X Q. Cinnamaldehyde improved intestine immune function and alleviated inflammation associated with NF-κB pathways in grass carp(Ctenopharyngodon idella)after infection with Aeromonas hydrophila[J]. Aquaculture Reports, 2021, 21:100837.doi:10.1016/j.aqrep.2021.100837. doi: 10.1016/j.aqrep.2021.100837 URL
[39]	Sánchez-Margalet V, Martín-Romero C, Santos-Alvarez J, Goberna R, Najib S, Gonzalez-Yanes C. Role of leptin as an immunomodulator of blood mononuclear cells:Mechanisms of action[J]. Clinical and Experimental Immunology, 2003, 133(1):11-19.doi:10.1046/j.1365-2249.2003.02190.x. doi: 10.1046/j.1365-2249.2003.02190.x pmid: 12823272
[40]	Duggal P, Guo X, Haque R, Peterson K M, Ricklefs S, Mondal D, Alam F, Noor Z, Verkerke H P, Marie C, Leduc C A, Chua S C Jr, Myers M G Jr, Leibel R L, Houpt E, Gilchrist C A, Sher A, Porcella S F, Petri W A Jr. A mutation in the leptin receptor is associated with Entamoeba histolytica infection in children[J]. Journal of Clinical Investigation, 2011, 121(3):1191-1198.doi:10.1172/JCI45294. doi: 10.1172/JCI45294 pmid: 21393862
[41]	Mykoniatis A, Anton P M, Wlk M, Wang C C, Ungsunan L, Blüher S, Venihaki M, Simeonidis S, Zacks J, Zhao D Z, Sougioultzis S, Karalis K, Mantzoros C, Pothoulakis C. Leptin mediates Clostridium difficile toxin A-induced enteritis in mice[J]. Gastroenterology, 2003, 124(3):683-691.doi:10.1053/gast.2003.50101. doi: 10.1053/gast.2003.50101 pmid: 12612907
[42]	Madan R, Guo X, Naylor C, Buonomo E L, Mackay D, Noor Z, Concannon P, Scully K W, Pramoonjago P, Kolling G L, Warren C A, Duggal P, Petri W A Jr. Role of leptin-mediated colonic inflammation in defense against Clostridium difficile colitis[J]. Infection and Immunity, 2014, 82(1):341-349.doi:10.1128/IAI.00972-13. doi: 10.1128/IAI.00972-13 URL
[43]	Nagajyothi F, Zhaoa D Z, S Machado F S, Weiss L M, Schwartz G J, Desruisseaux M S, Zhao Y, Factor S M, Huang H, Albanese C, Teixeira M M, Scherer P E, Chua S C, Tanowitz H B. Crucial role of the central leptin receptor in murine Trypanosoma cruzi(Brazil strain)infection[J]. The Journal of Infectious Diseases, 2010, 202(7):1104-1113.doi:10.1086/656189. doi: 10.1086/656189 URL
[44]	Teixeira L, Correia A, Oliveira B M, Pinto A, Ferreira P G, Vilanova M. Modulation of leptin and leptin receptor expression in mice acutely infected with Neospora caninum[J]. Pathogens, 2020, 9(7):587.doi:10.3390/pathogens9070587. doi: 10.3390/pathogens9070587 URL
[45]	Du Y, Song Y F, Zhang X J, Luo Y, Zou W Y, Zhang J W, Fu J L. Leptin receptor deficiency protects mice against chronic cerebral hypoperfusion-induced neuroinflammation and white matter lesions[J]. Mediators of Inflammation, 2020, 2020:7974537.doi:10.1155/2020/7974537. doi: 10.1155/2020/7974537

选择文件类型/文献管理软件名称

选择包含的内容

东北林蛙LEPR基因的克隆及其在感染中的表达分析

Cloning and Expression Analysis of Rana dybowskii LEPR Gene During Infection

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 12

参考文献 45

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张娟香, 马小勇, 路建卫, 仁青吉, 罗胜伟, 肖光峰, 王有鹤, 马晓明, 喇永福, 郭宪, 褚敏, 阎萍, 梁春年. *牦牛FTL基因克隆、生物信息学及表达分析*[J]. 华北农学报, 2023, 38(6): 192-200.
[2]	赖章龙, 阮超岭, 肖伟, 刘彦斌, 俞兆曦, 刘凯, 赛清云, 田永华, 张锋, 杨瑞兰, 柳婷, 杨立强, 王玉涛, 连总强. *兰州鲇NPY基因的克隆和表达分析*[J]. 华北农学报, 2023, 38(5): 227-238.
[3]	闫尊强, 计亚楠, 王鹏飞, 张博, 石海霞, 滚双宝. *合作猪StAR基因克隆、组织表达及生物信息学分析*[J]. 华北农学报, 2023, 38(4): 190-196.
[4]	王川川, 母童, 李德生, 张迪, 李蕾蕾, 张娟, 顾亚玲. *荷斯坦奶牛FABP4基因结构与功能分析*[J]. 华北农学报, 2023, 38(3): 204-212.
[5]	腾海艳. *水稻Osp39基因的组织表达和亚细胞定位分析*[J]. 华北农学报, 2023, 38(3): 10-17.
[6]	范依琳, 赵丹, 马妍, 岳永起, 冯欣欣, 张姬越, 字向东, 熊显荣, 付伟, 熊燕. *牦牛ANXA5基因的克隆、序列分析及表达研究*[J]. 华北农学报, 2023, 38(2): 224-231.
[7]	周小南, 师丹丹, 丁燕玲, 张岩峰, 赵志艳, 康晓龙. *秦川牛CDS1基因克隆、分子特征及组织表达分析*[J]. 华北农学报, 2023, 38(1): 218-225.
[8]	李杰, 黄晓宇, 尚学峰, 杨姣姣, 张娟丽, 谢开会, 张亚丽, 闫尊强, 王鹏飞, 高小莉, 杨巧丽, 马艳萍, 滚双宝. FABP2基因在不同比例花椒籽饲喂育肥猪的组织差异表达、克隆及生物信息学分析[J]. 华北农学报, 2022, 37(6): 228-238.
[9]	杨满珍, 闵星宇, 杨璐瑜, 于海玲, 胡宇磊, 朱艳锦, 潘帮婷, 李键, 熊显荣. *牦牛KIF2A基因克隆及其在卵泡与卵母细胞发育过程的表达规律*[J]. 华北农学报, 2022, 37(6): 219-227.
[10]	孙海丽, 梁静, 王文佳, 丁位华, 王妮娜, 柳凯恒, 李成伟. *拟南芥液泡H⁺-ATP酶E1亚基基因AtTUF启动子的克隆及表达分析*[J]. 华北农学报, 2022, 37(6): 82-90.
[11]	陈美, 柴志欣, 武志娟, 王吉坤, 钟金城, 信金伟. *牦牛CTGF基因克隆、蛋白功能及组织表达分析*[J]. 华北农学报, 2021, 36(S1): 361-367.
[12]	邬建飞, 刘宇, 李恒, 荆天, 卢建远, 字向东. *牦牛DDIT3基因克隆及组织表达特性分析*[J]. 华北农学报, 2021, 36(5): 216-225.
[13]	李娟, 郑姚, 王利. *九龙牦牛AQP7基因鉴定及在各组织中的表达和定位*[J]. 华北农学报, 2021, 36(4): 218-223.
[14]	郑艺超, 张昊, 赵丹, 王凤茹, 刘西岗, 郭琳. 拟南芥转录因子MYB54的体外纯化及功能初探[J]. 华北农学报, 2021, 36(4): 47-55.
[15]	王江林, 王永, 孟庆勇, 朱江江, 林亚秋. *山羊KLF6的分子克隆及其时空表达特征分析*[J]. 华北农学报, 2021, 36(2): 226-232.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

东北林蛙LEPR基因的克隆及其在感染中的表达分析

Cloning and Expression Analysis of Rana dybowskii LEPR Gene During Infection

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 12

参考文献 45

相关文章 15

编辑推荐

Metrics

本文评价